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Analysis of the ways and methods of signaling pathways in regulating cell cycle of NIH3T3 at transcriptional level.

Chang C, Niu Z, Gu N, Zhao W, Wang G, Jia Y, Li D, Xu C - BMC Cell Biol. (2015)

Bottom Line: Newfound genes such as PKC, RAS, PP2A, NGR and PI3K etc. belong to the functional category of molecular mechanism of cancer, cyclins and cell cycle regulation HER-2 signaling in breast cancer signaling pathways.These newfound genes could promote DNA damage repairment and DNA replication progress, regulate the metabolism of protein, and maintain the cell cycle progression of NIH3T3 modulating the reported genes CCND1 and C-FOS.All of the aforementioned signaling pathways interacted with the cell cycle network, indicating that NIH3T3 cell cycle was regulated by a number of signaling pathways.

View Article: PubMed Central - PubMed

Affiliation: College of Life Science, Henan Normal University, No. 46, Construction East Road, Xinxiang, 453007, Henan Province, P. R. China. changcuifang@126.com.

ABSTRACT

Background: To analyze the ways and methods of signaling pathways in regulating cell cycle progression of NIH3T3 at transcriptional level, we modeled cell cycle of NIH3T3 and found that G1 phase of NIH3T3 cell cycle was at 5-15 h after synchronization, S phase at 15-21 h, G2 phase at 21-22 h, M phase at 22-25 h.

Results: Mouse Genome 430 2.0 microarray was used to detect the gene expression profiles of the model, and results showed remarkable changes in the expressions of 64 cell cycle genes and 960 genes associated with other physiological activity during the cell cycle of NIH3T3. For the next step, IPA software was used to analyze the physiological activities, cell cycle genes-associated signal transduction activities and their regulatory roles of these genes in cell cycle progression, and our results indicated that the reported genes were involved in 17 signaling pathways in the regulation of cell cycle progression. Newfound genes such as PKC, RAS, PP2A, NGR and PI3K etc. belong to the functional category of molecular mechanism of cancer, cyclins and cell cycle regulation HER-2 signaling in breast cancer signaling pathways. These newfound genes could promote DNA damage repairment and DNA replication progress, regulate the metabolism of protein, and maintain the cell cycle progression of NIH3T3 modulating the reported genes CCND1 and C-FOS.

Conclusion: All of the aforementioned signaling pathways interacted with the cell cycle network, indicating that NIH3T3 cell cycle was regulated by a number of signaling pathways.

No MeSH data available.


Related in: MedlinePlus

The Genes heat maps of physiological activity the genes involved at different time of cell cycle
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Fig4: The Genes heat maps of physiological activity the genes involved at different time of cell cycle

Mentions: The analysis of the cell cycle physiological activities, which involved the reported cell cycle genes at different points in time, demonstrated that “G1 phase” and “cell cycle progression” were stronger at 5 h after synchronization, “G1 phase” and “cell cycle progression” at 10 h, “G1/S transition” at 15 h, “S phase” and “cell cycle progression” at 18 h, “M phase” and “checkpoint” at 21 h, S phase, “M phase” and “cell cycle progression” at 21.5 h, “M phase” at 22 and 23.5 h, “M phase” and “separation” at 25 h. Overall, the physiological activities conformed with cell cycle progression at all these points in time (Fig. 4).Fig. 4


Analysis of the ways and methods of signaling pathways in regulating cell cycle of NIH3T3 at transcriptional level.

Chang C, Niu Z, Gu N, Zhao W, Wang G, Jia Y, Li D, Xu C - BMC Cell Biol. (2015)

The Genes heat maps of physiological activity the genes involved at different time of cell cycle
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4625951&req=5

Fig4: The Genes heat maps of physiological activity the genes involved at different time of cell cycle
Mentions: The analysis of the cell cycle physiological activities, which involved the reported cell cycle genes at different points in time, demonstrated that “G1 phase” and “cell cycle progression” were stronger at 5 h after synchronization, “G1 phase” and “cell cycle progression” at 10 h, “G1/S transition” at 15 h, “S phase” and “cell cycle progression” at 18 h, “M phase” and “checkpoint” at 21 h, S phase, “M phase” and “cell cycle progression” at 21.5 h, “M phase” at 22 and 23.5 h, “M phase” and “separation” at 25 h. Overall, the physiological activities conformed with cell cycle progression at all these points in time (Fig. 4).Fig. 4

Bottom Line: Newfound genes such as PKC, RAS, PP2A, NGR and PI3K etc. belong to the functional category of molecular mechanism of cancer, cyclins and cell cycle regulation HER-2 signaling in breast cancer signaling pathways.These newfound genes could promote DNA damage repairment and DNA replication progress, regulate the metabolism of protein, and maintain the cell cycle progression of NIH3T3 modulating the reported genes CCND1 and C-FOS.All of the aforementioned signaling pathways interacted with the cell cycle network, indicating that NIH3T3 cell cycle was regulated by a number of signaling pathways.

View Article: PubMed Central - PubMed

Affiliation: College of Life Science, Henan Normal University, No. 46, Construction East Road, Xinxiang, 453007, Henan Province, P. R. China. changcuifang@126.com.

ABSTRACT

Background: To analyze the ways and methods of signaling pathways in regulating cell cycle progression of NIH3T3 at transcriptional level, we modeled cell cycle of NIH3T3 and found that G1 phase of NIH3T3 cell cycle was at 5-15 h after synchronization, S phase at 15-21 h, G2 phase at 21-22 h, M phase at 22-25 h.

Results: Mouse Genome 430 2.0 microarray was used to detect the gene expression profiles of the model, and results showed remarkable changes in the expressions of 64 cell cycle genes and 960 genes associated with other physiological activity during the cell cycle of NIH3T3. For the next step, IPA software was used to analyze the physiological activities, cell cycle genes-associated signal transduction activities and their regulatory roles of these genes in cell cycle progression, and our results indicated that the reported genes were involved in 17 signaling pathways in the regulation of cell cycle progression. Newfound genes such as PKC, RAS, PP2A, NGR and PI3K etc. belong to the functional category of molecular mechanism of cancer, cyclins and cell cycle regulation HER-2 signaling in breast cancer signaling pathways. These newfound genes could promote DNA damage repairment and DNA replication progress, regulate the metabolism of protein, and maintain the cell cycle progression of NIH3T3 modulating the reported genes CCND1 and C-FOS.

Conclusion: All of the aforementioned signaling pathways interacted with the cell cycle network, indicating that NIH3T3 cell cycle was regulated by a number of signaling pathways.

No MeSH data available.


Related in: MedlinePlus