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Use of multilocus sequence typing to infer genetic diversity and population structure of Lactobacillus plantarum isolates from different sources.

Xu H, Liu W, Zhang W, Yu J, Song Y, Menhe B, Zhang H, Sun Z - BMC Microbiol. (2015)

Bottom Line: The results showed that 179 isolates and seven reference isolates could be assigned to 73 different sequence types (STs), forming ten clonal complexes (CCs) and 23 singletons.We deduced that L. plantarum isolates from the same ecological niches have similar genetic diversity and population structure.The MLST scheme presented in this study provides abundant sequence data for L. plantarum and enabled global comparisons of isolates associated with various environmental origins to be made.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, 010018, China. weiliangxhy@163.com.

ABSTRACT

Background: Lactobacillus plantarum is a lactic acid bacterium (LAB) of considerable industrial interest since it has an important role in the production of fermented food. In the present study, the genetic diversity and population structure within 186 L. plantarum isolates was determined based on a novel MLST scheme employing eight housekeeping genes. These isolates had originated from different sources and geographic regions: 179 isolates were from our own culture collection and originated from China and Mongolia and seven isolates were type or reference isolates from other collections.

Results: The results showed that 179 isolates and seven reference isolates could be assigned to 73 different sequence types (STs), forming ten clonal complexes (CCs) and 23 singletons. There were 158 polymorphic sites detected in total, and the nucleotide diversity per site varied from 0.00401 in clpX to 0.03220 in groEL. The minimum spanning tree analyses suggested that the evolution of L. plantarum isolates have little relationship with ecological sources have similar nucleotide diversity. Phylogenetic trees and structure indicated that there were six lineages in the L. plantarum isolates used in our study. Split-decomposition and ClonalFrame analysis indicated that recombination had occurred throughout the population of L. plantarum, but it occurred at a low frequency in these eight loci.

Conclusion: We deduced that L. plantarum isolates from the same ecological niches have similar genetic diversity and population structure. The MLST scheme presented in this study provides abundant sequence data for L. plantarum and enabled global comparisons of isolates associated with various environmental origins to be made. This will further advance our understanding of the microbial ecology of this industrially important LAB.

No MeSH data available.


Split decomposition analysis based on all eight genes used in MLST
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Fig3: Split decomposition analysis based on all eight genes used in MLST

Mentions: Split decomposition analysis of the concatenated clpX, groEL, murC, murE, phesS, pyrG, recA and uvrC gene sequence fragments displayed in a split decomposition tree (Fig. 3) showed a network-like structure with multiple parallel paths. There were six distinct clusters in the split decomposition tree, which was consistent with the phylogeny observed previously in the neighbour-joining tree and from STRUCTURE analysis.Fig. 3


Use of multilocus sequence typing to infer genetic diversity and population structure of Lactobacillus plantarum isolates from different sources.

Xu H, Liu W, Zhang W, Yu J, Song Y, Menhe B, Zhang H, Sun Z - BMC Microbiol. (2015)

Split decomposition analysis based on all eight genes used in MLST
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4625847&req=5

Fig3: Split decomposition analysis based on all eight genes used in MLST
Mentions: Split decomposition analysis of the concatenated clpX, groEL, murC, murE, phesS, pyrG, recA and uvrC gene sequence fragments displayed in a split decomposition tree (Fig. 3) showed a network-like structure with multiple parallel paths. There were six distinct clusters in the split decomposition tree, which was consistent with the phylogeny observed previously in the neighbour-joining tree and from STRUCTURE analysis.Fig. 3

Bottom Line: The results showed that 179 isolates and seven reference isolates could be assigned to 73 different sequence types (STs), forming ten clonal complexes (CCs) and 23 singletons.We deduced that L. plantarum isolates from the same ecological niches have similar genetic diversity and population structure.The MLST scheme presented in this study provides abundant sequence data for L. plantarum and enabled global comparisons of isolates associated with various environmental origins to be made.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, 010018, China. weiliangxhy@163.com.

ABSTRACT

Background: Lactobacillus plantarum is a lactic acid bacterium (LAB) of considerable industrial interest since it has an important role in the production of fermented food. In the present study, the genetic diversity and population structure within 186 L. plantarum isolates was determined based on a novel MLST scheme employing eight housekeeping genes. These isolates had originated from different sources and geographic regions: 179 isolates were from our own culture collection and originated from China and Mongolia and seven isolates were type or reference isolates from other collections.

Results: The results showed that 179 isolates and seven reference isolates could be assigned to 73 different sequence types (STs), forming ten clonal complexes (CCs) and 23 singletons. There were 158 polymorphic sites detected in total, and the nucleotide diversity per site varied from 0.00401 in clpX to 0.03220 in groEL. The minimum spanning tree analyses suggested that the evolution of L. plantarum isolates have little relationship with ecological sources have similar nucleotide diversity. Phylogenetic trees and structure indicated that there were six lineages in the L. plantarum isolates used in our study. Split-decomposition and ClonalFrame analysis indicated that recombination had occurred throughout the population of L. plantarum, but it occurred at a low frequency in these eight loci.

Conclusion: We deduced that L. plantarum isolates from the same ecological niches have similar genetic diversity and population structure. The MLST scheme presented in this study provides abundant sequence data for L. plantarum and enabled global comparisons of isolates associated with various environmental origins to be made. This will further advance our understanding of the microbial ecology of this industrially important LAB.

No MeSH data available.