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Identification and Epigenetic Analysis of a Maternally Imprinted Gene Qpct.

Guo J, He H, Liu Q, Zhang F, Lv J, Zeng T, Gu N, Wu Q - Mol. Cells (2015)

Bottom Line: Our results show that Qpct is widely expressed during early embryonic development and can be detected in the telecephalon, midbrain, and rhombencephalon at E9.5b-E11.5.Moreover, Qpct is strikingly expressed in the brain, lung and liver in E15.5.Expression signals for Qpct achieved a peak at E15.5 during placental development and were only detected in the labyrinth layer in E15.5 placenta.

View Article: PubMed Central - PubMed

Affiliation: School of Life Science and Technology, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 150001, Heilongjiang, China.

ABSTRACT
Most imprinted genes are concerned with embryonic development, especially placental development. Here, we identified a placenta-specific imprinted gene Qpct. Our results show that Qpct is widely expressed during early embryonic development and can be detected in the telecephalon, midbrain, and rhombencephalon at E9.5b-E11.5. Moreover, Qpct is strikingly expressed in the brain, lung and liver in E15.5. Expression signals for Qpct achieved a peak at E15.5 during placental development and were only detected in the labyrinth layer in E15.5 placenta. ChIP assay results suggest that the modification of histone H3K4me3 can result in maternal activating of Qpct.

No MeSH data available.


Genomic structure diagram and imprinting analysis of Qpct gene in mouse embryo and placenta. (A) Brown-red, exon; black vertical line, intron; blue horizontal line, position of methylation analysis primers; green horizontal line, position of ChIP analysis primers; yellow horizontal line, position of imprinting analysis primers; TSS, transcriptional start site. (B) Sequencing of genomic DNA from E15.5 C57BL/6 and DBA embryos, placenta and chorionic plate from hybrid F1 is heterozygous for polymorphism C/T. Sequencing of cDNA from hybrid F1 proves maternally biased expression of Qpct in E15.5 placenta and chorionic plate.
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f1-molce-38-10-859: Genomic structure diagram and imprinting analysis of Qpct gene in mouse embryo and placenta. (A) Brown-red, exon; black vertical line, intron; blue horizontal line, position of methylation analysis primers; green horizontal line, position of ChIP analysis primers; yellow horizontal line, position of imprinting analysis primers; TSS, transcriptional start site. (B) Sequencing of genomic DNA from E15.5 C57BL/6 and DBA embryos, placenta and chorionic plate from hybrid F1 is heterozygous for polymorphism C/T. Sequencing of cDNA from hybrid F1 proves maternally biased expression of Qpct in E15.5 placenta and chorionic plate.

Mentions: The mouse Qpct gene has seven exons in the mouse genome, and its full-length DNA and mRNA span 38 kb and 1.9 kb, respectively. We retrieved a CpG island of 197 bp in length, overlapping the exon 1 and the intron 1 of Qpct from the UCSC Genome Browser (Fig. 1A). A DNA polymorphism (T/C) at exon 7 was detected between C57BL/6 and DBA. The polymorphic site was used to distinguish monoallelic and biallelic expression. The results showed that Qpct was exclusively maternal allele-specifically expressed in the placenta and chorionic plate at E15.5 (Fig. 1B).


Identification and Epigenetic Analysis of a Maternally Imprinted Gene Qpct.

Guo J, He H, Liu Q, Zhang F, Lv J, Zeng T, Gu N, Wu Q - Mol. Cells (2015)

Genomic structure diagram and imprinting analysis of Qpct gene in mouse embryo and placenta. (A) Brown-red, exon; black vertical line, intron; blue horizontal line, position of methylation analysis primers; green horizontal line, position of ChIP analysis primers; yellow horizontal line, position of imprinting analysis primers; TSS, transcriptional start site. (B) Sequencing of genomic DNA from E15.5 C57BL/6 and DBA embryos, placenta and chorionic plate from hybrid F1 is heterozygous for polymorphism C/T. Sequencing of cDNA from hybrid F1 proves maternally biased expression of Qpct in E15.5 placenta and chorionic plate.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4625067&req=5

f1-molce-38-10-859: Genomic structure diagram and imprinting analysis of Qpct gene in mouse embryo and placenta. (A) Brown-red, exon; black vertical line, intron; blue horizontal line, position of methylation analysis primers; green horizontal line, position of ChIP analysis primers; yellow horizontal line, position of imprinting analysis primers; TSS, transcriptional start site. (B) Sequencing of genomic DNA from E15.5 C57BL/6 and DBA embryos, placenta and chorionic plate from hybrid F1 is heterozygous for polymorphism C/T. Sequencing of cDNA from hybrid F1 proves maternally biased expression of Qpct in E15.5 placenta and chorionic plate.
Mentions: The mouse Qpct gene has seven exons in the mouse genome, and its full-length DNA and mRNA span 38 kb and 1.9 kb, respectively. We retrieved a CpG island of 197 bp in length, overlapping the exon 1 and the intron 1 of Qpct from the UCSC Genome Browser (Fig. 1A). A DNA polymorphism (T/C) at exon 7 was detected between C57BL/6 and DBA. The polymorphic site was used to distinguish monoallelic and biallelic expression. The results showed that Qpct was exclusively maternal allele-specifically expressed in the placenta and chorionic plate at E15.5 (Fig. 1B).

Bottom Line: Our results show that Qpct is widely expressed during early embryonic development and can be detected in the telecephalon, midbrain, and rhombencephalon at E9.5b-E11.5.Moreover, Qpct is strikingly expressed in the brain, lung and liver in E15.5.Expression signals for Qpct achieved a peak at E15.5 during placental development and were only detected in the labyrinth layer in E15.5 placenta.

View Article: PubMed Central - PubMed

Affiliation: School of Life Science and Technology, State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, 150001, Heilongjiang, China.

ABSTRACT
Most imprinted genes are concerned with embryonic development, especially placental development. Here, we identified a placenta-specific imprinted gene Qpct. Our results show that Qpct is widely expressed during early embryonic development and can be detected in the telecephalon, midbrain, and rhombencephalon at E9.5b-E11.5. Moreover, Qpct is strikingly expressed in the brain, lung and liver in E15.5. Expression signals for Qpct achieved a peak at E15.5 during placental development and were only detected in the labyrinth layer in E15.5 placenta. ChIP assay results suggest that the modification of histone H3K4me3 can result in maternal activating of Qpct.

No MeSH data available.