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Smooth muscle cells of penis in the rat: noninvasive quantification with shear wave elastography.

Zhang JJ, Qiao XH, Gao F, Bai M, Li F, Du LF, Xing JF - Biomed Res Int (2015)

Bottom Line: The results showed that TS was significantly correlated with AP and the correlation coefficient was -0.618 (p < 0.001).The result of TS had been plotted against the AP measurements.The level of SMCs in penis was successfully quantified in vivo with SWE.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Ultrasound, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.

ABSTRACT

Purpose: Smooth muscle cells (SMCs) of cavernosum play an important role in erection. It is of great significance to quantitatively analyze the level of SMCs in penis. In this study, we investigated the feasibility of shear wave elastography (SWE) on evaluating the level of SMCs in penis quantitatively.

Materials and methods: Twenty healthy male rats were selected. The SWE imaging of penis was carried out and then immunohistochemistry analysis of penis was performed to analyze the expression of alpha smooth muscle actin in penis. The measurement index of SWE examination was tissue stiffness (TS). The measurement index of immunohistochemistry analysis was positive area percentage of alpha smooth muscle actin (AP).

Results: Sixty sets of data of TS and AP were obtained. The results showed that TS was significantly correlated with AP and the correlation coefficient was -0.618 (p < 0.001). The result of TS had been plotted against the AP measurements. The relation between the two results has been fitted with quadric curve; the goodness-of-fit index was 0.364 (p < 0.001).

Conclusions: The level of SMCs in penis was successfully quantified in vivo with SWE. SWE can be used clinically for evaluating the level of SMCs in penis quantitatively.

No MeSH data available.


Related in: MedlinePlus

The result of TS plotted against the AP measurements of each rat. The relation between the two results has been fitted with quadric curve.
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fig4: The result of TS plotted against the AP measurements of each rat. The relation between the two results has been fitted with quadric curve.

Mentions: For each rat, the near glans, mid, and near root segment of penis were, respectively, selected for the measurement of TS and histological examinations. Sixty sets of data of TS and AP were obtained. We found that the data of TS followed normal distribution (p = 0.909), but the data of AP did not. So the Spearman test was used to analyze the correlation between TS and AP. The result showed that TS was significantly correlated with AP (p < 0.001); the correlation coefficient was −0.618. The result of TS has been plotted against the AP measurements of each rat (Figure 4). The relation between the two results has been fitted with quadric curve (y = 0.001x2 − 0.132x + 10.349); the goodness-of-fit index was 0.364 (p < 0.001).


Smooth muscle cells of penis in the rat: noninvasive quantification with shear wave elastography.

Zhang JJ, Qiao XH, Gao F, Bai M, Li F, Du LF, Xing JF - Biomed Res Int (2015)

The result of TS plotted against the AP measurements of each rat. The relation between the two results has been fitted with quadric curve.
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4624877&req=5

fig4: The result of TS plotted against the AP measurements of each rat. The relation between the two results has been fitted with quadric curve.
Mentions: For each rat, the near glans, mid, and near root segment of penis were, respectively, selected for the measurement of TS and histological examinations. Sixty sets of data of TS and AP were obtained. We found that the data of TS followed normal distribution (p = 0.909), but the data of AP did not. So the Spearman test was used to analyze the correlation between TS and AP. The result showed that TS was significantly correlated with AP (p < 0.001); the correlation coefficient was −0.618. The result of TS has been plotted against the AP measurements of each rat (Figure 4). The relation between the two results has been fitted with quadric curve (y = 0.001x2 − 0.132x + 10.349); the goodness-of-fit index was 0.364 (p < 0.001).

Bottom Line: The results showed that TS was significantly correlated with AP and the correlation coefficient was -0.618 (p < 0.001).The result of TS had been plotted against the AP measurements.The level of SMCs in penis was successfully quantified in vivo with SWE.

View Article: PubMed Central - PubMed

Affiliation: Department of Medical Ultrasound, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.

ABSTRACT

Purpose: Smooth muscle cells (SMCs) of cavernosum play an important role in erection. It is of great significance to quantitatively analyze the level of SMCs in penis. In this study, we investigated the feasibility of shear wave elastography (SWE) on evaluating the level of SMCs in penis quantitatively.

Materials and methods: Twenty healthy male rats were selected. The SWE imaging of penis was carried out and then immunohistochemistry analysis of penis was performed to analyze the expression of alpha smooth muscle actin in penis. The measurement index of SWE examination was tissue stiffness (TS). The measurement index of immunohistochemistry analysis was positive area percentage of alpha smooth muscle actin (AP).

Results: Sixty sets of data of TS and AP were obtained. The results showed that TS was significantly correlated with AP and the correlation coefficient was -0.618 (p < 0.001). The result of TS had been plotted against the AP measurements. The relation between the two results has been fitted with quadric curve; the goodness-of-fit index was 0.364 (p < 0.001).

Conclusions: The level of SMCs in penis was successfully quantified in vivo with SWE. SWE can be used clinically for evaluating the level of SMCs in penis quantitatively.

No MeSH data available.


Related in: MedlinePlus