Limits...
CCR7(lo)PD-1(hi) CXCR5(+) CD4(+) T cells are positively correlated with levels of IL-21 in active and transitional cystic echinococcosis patients.

Zhang F, Pang N, Zhu Y, Zhou D, Zhao H, Hu J, Ma X, Li J, Wen H, Samten B, Fan H, Ding J - BMC Infect. Dis. (2015)

Bottom Line: Percentages of circulating Tfh cells were significantly increased in the CE1, CE2, and CE3 groups (p < 0.05).Additionally, after stimulation with hydatid fluid in vitro, the levels of circulating Tfh cells, IL-21 and IL-4 in PBMCs isolated from CE patients were significantly increased (p < 0.05).The levels of circulating Tfh and related cytokines were significantly increased in CE patients, suggesting that they are involved in human CE.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Xinjiang Medical University, 393 Xinyi Road, 830011, Urumqi, Xinjiang, China. 765219598@qq.com.

ABSTRACT

Background: In our study, we investigated whether circulating T follicular helper (Tfh) and the related cytokines are involved in human cystic echinococcosis (CE).

Methods: A total of 64 patients with CE and 30 healthy controls were enrolled in this study. Percentages of CCR7(lo)PD-1(hi) cells within CXCR5(+) CD4(+) T cells (circulating Tfh cells) were detected by flow cytometry. Levels of IL-21 and IL-4 in peripheral blood were detected by cytometric bead array. The mRNA expression of IL-21, IL-4, Bcl-6, and Blimp-1 in peripheral blood mononuclear cells (PBMCs) were measured by real-time PCR. Levels of IgG1, IgG2, IgG3, and IgG4 in the patients' sera were measured using enzyme-linked immunosorbent assay.

Results: Percentages of circulating Tfh cells were significantly increased in the CE1, CE2, and CE3 groups (p < 0.05). The concentrations of IL-21 and IL-4 in the serum were significantly increased in CE1, CE2, and CE3 groups (p < 0.05). IL-21 was positively correlated with circulating Tfh cells in CE3 group (r = 0.779, p < 0.05). The mRNA levels of IL-21, IL-4, and Bcl-6 were increased in CE1, CE2, and CE3 groups. Levels of IgG1 and IgG4 in patients' sera were increased in CE1, CE2, and CE3 groups. Levels of IgG2 and IgG3 were increased in CE4-5 group. Additionally, after stimulation with hydatid fluid in vitro, the levels of circulating Tfh cells, IL-21 and IL-4 in PBMCs isolated from CE patients were significantly increased (p < 0.05).

Conclusions: The levels of circulating Tfh and related cytokines were significantly increased in CE patients, suggesting that they are involved in human CE.

No MeSH data available.


Related in: MedlinePlus

Increased frequency of CCR7loPD-1hi cells within CXCR5+ CD4+ T cells in CE patients. The PBMCs were stained with Vioblue-CD3, PERCP-CD4, PE-cy7-CCR7, FITC-CXCR5 and APC-CD45RA. The percentages of CCR7loPD-1hi cells within CXCR5+CD4+ T cells were determined by flow cytometry analysis. a Representative flow cytometry dot-plots of the percent of CD45RA−CXCR5+CD4+ T cells. b Quantification of phenotypic marker CCR7 and PD-1 in each group. c The statistical results of percentage of CCR7loPD-1hi within CXCR5+ CD4+ T cells in each group
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
getmorefigures.php?uid=PMC4624577&req=5

Fig1: Increased frequency of CCR7loPD-1hi cells within CXCR5+ CD4+ T cells in CE patients. The PBMCs were stained with Vioblue-CD3, PERCP-CD4, PE-cy7-CCR7, FITC-CXCR5 and APC-CD45RA. The percentages of CCR7loPD-1hi cells within CXCR5+CD4+ T cells were determined by flow cytometry analysis. a Representative flow cytometry dot-plots of the percent of CD45RA−CXCR5+CD4+ T cells. b Quantification of phenotypic marker CCR7 and PD-1 in each group. c The statistical results of percentage of CCR7loPD-1hi within CXCR5+ CD4+ T cells in each group

Mentions: To determine expression of CCR7loPD-1hi T cells in PBMCs from CE patients, flow cytometry analysis was performed. CD45RA was used to identify the effector/memory T cells (CD45RA−) in CD3+CD4+ T cells and the cells positive for CXCR5 was further analyzed for the percentage of Tfh cells expressing CCR7loPD-1hi (Fig. 1). The results showed that the percentages of CCR7loPD-1hi cells within CXCR5+ CD4+ T cells in CE1 (33.14 % ± 3.35), CE2 (34.58 % ± 4.00), and CE3 (31.95 % ± 4.84) group were significantly increased (p < 0.05) compared with those in the healthy controls (13.54 % ± 3.89) and in CE4-5 types (15.06 % ± 5.22). However, difference between CE4-5 group and the healthy control group was not statistically significant. Differences among CE1, CE2, and CE3 groups were also not statistically significant. These results indicated that percentages of CCR7loPD-1hi cells within CXCR5+ CD4+ T cells are significantly higher in CE1, CE2, and CE3 group patients than in healthy controls.Fig. 1


CCR7(lo)PD-1(hi) CXCR5(+) CD4(+) T cells are positively correlated with levels of IL-21 in active and transitional cystic echinococcosis patients.

Zhang F, Pang N, Zhu Y, Zhou D, Zhao H, Hu J, Ma X, Li J, Wen H, Samten B, Fan H, Ding J - BMC Infect. Dis. (2015)

Increased frequency of CCR7loPD-1hi cells within CXCR5+ CD4+ T cells in CE patients. The PBMCs were stained with Vioblue-CD3, PERCP-CD4, PE-cy7-CCR7, FITC-CXCR5 and APC-CD45RA. The percentages of CCR7loPD-1hi cells within CXCR5+CD4+ T cells were determined by flow cytometry analysis. a Representative flow cytometry dot-plots of the percent of CD45RA−CXCR5+CD4+ T cells. b Quantification of phenotypic marker CCR7 and PD-1 in each group. c The statistical results of percentage of CCR7loPD-1hi within CXCR5+ CD4+ T cells in each group
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4624577&req=5

Fig1: Increased frequency of CCR7loPD-1hi cells within CXCR5+ CD4+ T cells in CE patients. The PBMCs were stained with Vioblue-CD3, PERCP-CD4, PE-cy7-CCR7, FITC-CXCR5 and APC-CD45RA. The percentages of CCR7loPD-1hi cells within CXCR5+CD4+ T cells were determined by flow cytometry analysis. a Representative flow cytometry dot-plots of the percent of CD45RA−CXCR5+CD4+ T cells. b Quantification of phenotypic marker CCR7 and PD-1 in each group. c The statistical results of percentage of CCR7loPD-1hi within CXCR5+ CD4+ T cells in each group
Mentions: To determine expression of CCR7loPD-1hi T cells in PBMCs from CE patients, flow cytometry analysis was performed. CD45RA was used to identify the effector/memory T cells (CD45RA−) in CD3+CD4+ T cells and the cells positive for CXCR5 was further analyzed for the percentage of Tfh cells expressing CCR7loPD-1hi (Fig. 1). The results showed that the percentages of CCR7loPD-1hi cells within CXCR5+ CD4+ T cells in CE1 (33.14 % ± 3.35), CE2 (34.58 % ± 4.00), and CE3 (31.95 % ± 4.84) group were significantly increased (p < 0.05) compared with those in the healthy controls (13.54 % ± 3.89) and in CE4-5 types (15.06 % ± 5.22). However, difference between CE4-5 group and the healthy control group was not statistically significant. Differences among CE1, CE2, and CE3 groups were also not statistically significant. These results indicated that percentages of CCR7loPD-1hi cells within CXCR5+ CD4+ T cells are significantly higher in CE1, CE2, and CE3 group patients than in healthy controls.Fig. 1

Bottom Line: Percentages of circulating Tfh cells were significantly increased in the CE1, CE2, and CE3 groups (p < 0.05).Additionally, after stimulation with hydatid fluid in vitro, the levels of circulating Tfh cells, IL-21 and IL-4 in PBMCs isolated from CE patients were significantly increased (p < 0.05).The levels of circulating Tfh and related cytokines were significantly increased in CE patients, suggesting that they are involved in human CE.

View Article: PubMed Central - PubMed

Affiliation: Department of Clinical Laboratory, First Affiliated Hospital of Xinjiang Medical University, 393 Xinyi Road, 830011, Urumqi, Xinjiang, China. 765219598@qq.com.

ABSTRACT

Background: In our study, we investigated whether circulating T follicular helper (Tfh) and the related cytokines are involved in human cystic echinococcosis (CE).

Methods: A total of 64 patients with CE and 30 healthy controls were enrolled in this study. Percentages of CCR7(lo)PD-1(hi) cells within CXCR5(+) CD4(+) T cells (circulating Tfh cells) were detected by flow cytometry. Levels of IL-21 and IL-4 in peripheral blood were detected by cytometric bead array. The mRNA expression of IL-21, IL-4, Bcl-6, and Blimp-1 in peripheral blood mononuclear cells (PBMCs) were measured by real-time PCR. Levels of IgG1, IgG2, IgG3, and IgG4 in the patients' sera were measured using enzyme-linked immunosorbent assay.

Results: Percentages of circulating Tfh cells were significantly increased in the CE1, CE2, and CE3 groups (p < 0.05). The concentrations of IL-21 and IL-4 in the serum were significantly increased in CE1, CE2, and CE3 groups (p < 0.05). IL-21 was positively correlated with circulating Tfh cells in CE3 group (r = 0.779, p < 0.05). The mRNA levels of IL-21, IL-4, and Bcl-6 were increased in CE1, CE2, and CE3 groups. Levels of IgG1 and IgG4 in patients' sera were increased in CE1, CE2, and CE3 groups. Levels of IgG2 and IgG3 were increased in CE4-5 group. Additionally, after stimulation with hydatid fluid in vitro, the levels of circulating Tfh cells, IL-21 and IL-4 in PBMCs isolated from CE patients were significantly increased (p < 0.05).

Conclusions: The levels of circulating Tfh and related cytokines were significantly increased in CE patients, suggesting that they are involved in human CE.

No MeSH data available.


Related in: MedlinePlus