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Transcriptome profile analysis of cell proliferation molecular processes during multicellular trichome formation induced by tomato Wov gene in tobacco.

Yang C, Gao Y, Gao S, Yu G, Xiong C, Chang J, Li H, Ye Z - BMC Genomics (2015)

Bottom Line: In addition, the transcriptional levels of homologues of trichome regulators in Rosids were not significantly changed, whereas homologues of genes (Wo and SlCycB2) in Asterids were significantly upregulated in Wov transgenic tobacco plants.And the results provided us new insight into the molecular processes controlling multicellular formation in tobacco.Furthermore, we inferred that trichomes in solanaceous species might share a common network.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, Hubei, PR China. yangcx0915@mail.hzau.edu.cn.

ABSTRACT

Background: Trichomes, developing from the epidermis of nearly all terrestrial plants, provide good structural resistance against insect herbivores and an excellent model for studying the molecular mechanisms underlying cell fate determination. Regulation of trichomes in Rosids has been well characterized. However, little is known about the cell proliferation molecular processes during multicellular trichome formation in Asterids.

Results: In this study, we identified two point mutations in a novel allele (Wov) at Wo locus. Ectopic expression of Wov in tobacco and potato induces much more trichome formation than wild type. To gain new insights into the underlying mechanisms during the processes of these trichomes formation, we compared the gene expression profiles between Wov transgenic and wild-type tobacco by RNA-seq analysis. A total of 544 co-DEGs were detected between transgenic and wild-type tobacco. Functional assignments of the co-DEGs indicated that 33 reliable pathways are altered in transgenic tobacco plants. The most noticeable pathways are fatty acid metabolism, amino acid biosynthesis and metabolism, and plant hormone signal transduction. Results suggest that these enhanced processes are critical for the cell proliferation during multicellular trichome formation in transgenic plants. In addition, the transcriptional levels of homologues of trichome regulators in Rosids were not significantly changed, whereas homologues of genes (Wo and SlCycB2) in Asterids were significantly upregulated in Wov transgenic tobacco plants.

Conclusions: This study presents a global picture of the gene expression changes induced by Wov-gene in tobacco. And the results provided us new insight into the molecular processes controlling multicellular formation in tobacco. Furthermore, we inferred that trichomes in solanaceous species might share a common network.

No MeSH data available.


Related in: MedlinePlus

Real-time Q-RT-PCR confirmation of the differentially expressed genes between the wild type (grey columns) and the Wov transgenic tobacco plants (black columns). Columns and bars represent the means and standard error (n = 3) respectively. The transcript abundance from RNA-seq data was displayed on the top of each gene
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Fig7: Real-time Q-RT-PCR confirmation of the differentially expressed genes between the wild type (grey columns) and the Wov transgenic tobacco plants (black columns). Columns and bars represent the means and standard error (n = 3) respectively. The transcript abundance from RNA-seq data was displayed on the top of each gene

Mentions: To confirm the RNA-seq results, quantitative RT-PCR was conducted on the randomly selected Wov-related genes based on the RNA-seq data. Transcriptional change revealed by RNA-seq was also validated in independent experiments. A total of 28 significantly differentially expressed genes, including 14 up-regulated genes and 14 down-regulated genes, were selected to design the gene-specific primers (Additional file 2). The patterns of transcript abundance between WT and T were compared with RNA-seq data. Although some quantitative differences exist at expression level, qRT-PCR results indicated that all of the 28 genes displayed the similar expression tendency as the RNA-seq data. Figure 6 shows the expression levels of the 28 genes between MT and T. For instance, the transcript level of germin-like gene_80918 was up-regulated 62.6 times more in MT than in T as analyzed by quantitative RT-PCR, which was consistent with RNA-seq data demonstrating that the gene expression in the T was 8.9-fold higher than that in the WT. The expression level of the rest of the genes, including defensin-like gene, osmotin 34, serine protease inhibitor, RING/U-box gene, SAUR-like auxin-responsive gene, and six other newly detected genes with significant transcriptional changes without homologues in Arabidopsis (gene_60204, gene_69530, gene_47998, gene_46949, gene_8837, and gene_48080), were analyzed between the WT and transgenic plants (Fig. 7). As expected, the transcription levels of defensin-like gene, osmotin 34, and serine protease inhibitor were significantly upregulated in T versus WT, whereas those of RING/U-box and SAUR-like auxin-responsive genes were significantly downregulated in T compared with WT. These findings correlate well with the RNA-seq data. Notably, the expression level of the three homologs of tomato trichome-related genes (gene_2092, gene_49227, and gene_22166) in T is higher than that in WT, which showed a very good correlation with the RNA-seq results (Fig. 8).Fig. 7


Transcriptome profile analysis of cell proliferation molecular processes during multicellular trichome formation induced by tomato Wov gene in tobacco.

Yang C, Gao Y, Gao S, Yu G, Xiong C, Chang J, Li H, Ye Z - BMC Genomics (2015)

Real-time Q-RT-PCR confirmation of the differentially expressed genes between the wild type (grey columns) and the Wov transgenic tobacco plants (black columns). Columns and bars represent the means and standard error (n = 3) respectively. The transcript abundance from RNA-seq data was displayed on the top of each gene
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4623907&req=5

Fig7: Real-time Q-RT-PCR confirmation of the differentially expressed genes between the wild type (grey columns) and the Wov transgenic tobacco plants (black columns). Columns and bars represent the means and standard error (n = 3) respectively. The transcript abundance from RNA-seq data was displayed on the top of each gene
Mentions: To confirm the RNA-seq results, quantitative RT-PCR was conducted on the randomly selected Wov-related genes based on the RNA-seq data. Transcriptional change revealed by RNA-seq was also validated in independent experiments. A total of 28 significantly differentially expressed genes, including 14 up-regulated genes and 14 down-regulated genes, were selected to design the gene-specific primers (Additional file 2). The patterns of transcript abundance between WT and T were compared with RNA-seq data. Although some quantitative differences exist at expression level, qRT-PCR results indicated that all of the 28 genes displayed the similar expression tendency as the RNA-seq data. Figure 6 shows the expression levels of the 28 genes between MT and T. For instance, the transcript level of germin-like gene_80918 was up-regulated 62.6 times more in MT than in T as analyzed by quantitative RT-PCR, which was consistent with RNA-seq data demonstrating that the gene expression in the T was 8.9-fold higher than that in the WT. The expression level of the rest of the genes, including defensin-like gene, osmotin 34, serine protease inhibitor, RING/U-box gene, SAUR-like auxin-responsive gene, and six other newly detected genes with significant transcriptional changes without homologues in Arabidopsis (gene_60204, gene_69530, gene_47998, gene_46949, gene_8837, and gene_48080), were analyzed between the WT and transgenic plants (Fig. 7). As expected, the transcription levels of defensin-like gene, osmotin 34, and serine protease inhibitor were significantly upregulated in T versus WT, whereas those of RING/U-box and SAUR-like auxin-responsive genes were significantly downregulated in T compared with WT. These findings correlate well with the RNA-seq data. Notably, the expression level of the three homologs of tomato trichome-related genes (gene_2092, gene_49227, and gene_22166) in T is higher than that in WT, which showed a very good correlation with the RNA-seq results (Fig. 8).Fig. 7

Bottom Line: In addition, the transcriptional levels of homologues of trichome regulators in Rosids were not significantly changed, whereas homologues of genes (Wo and SlCycB2) in Asterids were significantly upregulated in Wov transgenic tobacco plants.And the results provided us new insight into the molecular processes controlling multicellular formation in tobacco.Furthermore, we inferred that trichomes in solanaceous species might share a common network.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, Hubei, PR China. yangcx0915@mail.hzau.edu.cn.

ABSTRACT

Background: Trichomes, developing from the epidermis of nearly all terrestrial plants, provide good structural resistance against insect herbivores and an excellent model for studying the molecular mechanisms underlying cell fate determination. Regulation of trichomes in Rosids has been well characterized. However, little is known about the cell proliferation molecular processes during multicellular trichome formation in Asterids.

Results: In this study, we identified two point mutations in a novel allele (Wov) at Wo locus. Ectopic expression of Wov in tobacco and potato induces much more trichome formation than wild type. To gain new insights into the underlying mechanisms during the processes of these trichomes formation, we compared the gene expression profiles between Wov transgenic and wild-type tobacco by RNA-seq analysis. A total of 544 co-DEGs were detected between transgenic and wild-type tobacco. Functional assignments of the co-DEGs indicated that 33 reliable pathways are altered in transgenic tobacco plants. The most noticeable pathways are fatty acid metabolism, amino acid biosynthesis and metabolism, and plant hormone signal transduction. Results suggest that these enhanced processes are critical for the cell proliferation during multicellular trichome formation in transgenic plants. In addition, the transcriptional levels of homologues of trichome regulators in Rosids were not significantly changed, whereas homologues of genes (Wo and SlCycB2) in Asterids were significantly upregulated in Wov transgenic tobacco plants.

Conclusions: This study presents a global picture of the gene expression changes induced by Wov-gene in tobacco. And the results provided us new insight into the molecular processes controlling multicellular formation in tobacco. Furthermore, we inferred that trichomes in solanaceous species might share a common network.

No MeSH data available.


Related in: MedlinePlus