Limits...
Differential Metabolic Profiles during the Albescent Stages of 'Anji Baicha' (Camellia sinensis).

Li CF, Yao MZ, Ma CL, Ma JQ, Jin JQ, Chen L - PLoS ONE (2015)

Bottom Line: A total of 65 differential metabolites were identified with a variable influence on projection greater than 1.The main differential metabolic pathways of the albescent stages compared with the re-greening stage included carbon fixation in photosynthetic organisms and the phenylpropanoid and flavonoid biosynthesis pathways.During the albescent stages, the sugar (fructofuranose), sugar derivative (glucose-1-phosphate) and epicatechin concentrations decreased, whereas the amino acid (mainly glycine, serine, tryptophan, citrulline, glutamine, proline, and valine) concentrations increased.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou, China.

ABSTRACT
'Anji Baicha' is an albino tea cultivar with white shoots at low air temperature and green shoots at high air temperature in early spring. The metabolite contents in the shoots dynamically vary with the color changes and with shoot development. To investigate the metabolomic variation during the albescent and re-greening stages, gas chromatography-mass spectrometry combined with multivariate analysis were applied to analyze the metabolite profiles in the different color stages during the development of 'Anji Baicha' leaves. The metabolite profiles of three albescent stages, including the yellow-green stage, the early albescent stage, and the late albescent stage, as well as the re-greening stage were distinguished using principal component analysis, revealing that the distinct developmental stages were likely responsible for the observed metabolic differences. Furthermore, a group classification and pairwise discrimination was revealed among the three albescent stages and re-greening stage by partial least squares discriminant analysis. A total of 65 differential metabolites were identified with a variable influence on projection greater than 1. The main differential metabolic pathways of the albescent stages compared with the re-greening stage included carbon fixation in photosynthetic organisms and the phenylpropanoid and flavonoid biosynthesis pathways. Compared with the re-greening stage, the carbohydrate and amino acid metabolic pathways were disturbed during the albescent stages. During the albescent stages, the sugar (fructofuranose), sugar derivative (glucose-1-phosphate) and epicatechin concentrations decreased, whereas the amino acid (mainly glycine, serine, tryptophan, citrulline, glutamine, proline, and valine) concentrations increased. These results reveal the changes in metabolic profiling that occur during the color changes associated with the development of the albino tea plant leaves.

No MeSH data available.


Related in: MedlinePlus

Differential metabolite contents in the YG, WI, WII and G stages of the tea plant.Differential metabolites identified in the distinct albescent stages compared with the G stage in this study are shown in bold. Red denotes the highest concentration, and green denotes the lowest concentration. The scale (ranging from 0–6) represents the log 10 (average value in each stage +1) of the differential metabolites in the YG, WI and WII stages compared with those in the G stage.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4622044&req=5

pone.0139996.g007: Differential metabolite contents in the YG, WI, WII and G stages of the tea plant.Differential metabolites identified in the distinct albescent stages compared with the G stage in this study are shown in bold. Red denotes the highest concentration, and green denotes the lowest concentration. The scale (ranging from 0–6) represents the log 10 (average value in each stage +1) of the differential metabolites in the YG, WI and WII stages compared with those in the G stage.

Mentions: Metabolic changes were observed during the YG, WI and WII stages compared with the G stage. The differential metabolites were mainly involved in carbohydrate and amino acid metabolism. The heat maps illustrate the concentrations of these differential metabolites in the YG, WI, WII and G stages using a log ratio (Fig 7 and S8 Table).


Differential Metabolic Profiles during the Albescent Stages of 'Anji Baicha' (Camellia sinensis).

Li CF, Yao MZ, Ma CL, Ma JQ, Jin JQ, Chen L - PLoS ONE (2015)

Differential metabolite contents in the YG, WI, WII and G stages of the tea plant.Differential metabolites identified in the distinct albescent stages compared with the G stage in this study are shown in bold. Red denotes the highest concentration, and green denotes the lowest concentration. The scale (ranging from 0–6) represents the log 10 (average value in each stage +1) of the differential metabolites in the YG, WI and WII stages compared with those in the G stage.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4622044&req=5

pone.0139996.g007: Differential metabolite contents in the YG, WI, WII and G stages of the tea plant.Differential metabolites identified in the distinct albescent stages compared with the G stage in this study are shown in bold. Red denotes the highest concentration, and green denotes the lowest concentration. The scale (ranging from 0–6) represents the log 10 (average value in each stage +1) of the differential metabolites in the YG, WI and WII stages compared with those in the G stage.
Mentions: Metabolic changes were observed during the YG, WI and WII stages compared with the G stage. The differential metabolites were mainly involved in carbohydrate and amino acid metabolism. The heat maps illustrate the concentrations of these differential metabolites in the YG, WI, WII and G stages using a log ratio (Fig 7 and S8 Table).

Bottom Line: A total of 65 differential metabolites were identified with a variable influence on projection greater than 1.The main differential metabolic pathways of the albescent stages compared with the re-greening stage included carbon fixation in photosynthetic organisms and the phenylpropanoid and flavonoid biosynthesis pathways.During the albescent stages, the sugar (fructofuranose), sugar derivative (glucose-1-phosphate) and epicatechin concentrations decreased, whereas the amino acid (mainly glycine, serine, tryptophan, citrulline, glutamine, proline, and valine) concentrations increased.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou, China.

ABSTRACT
'Anji Baicha' is an albino tea cultivar with white shoots at low air temperature and green shoots at high air temperature in early spring. The metabolite contents in the shoots dynamically vary with the color changes and with shoot development. To investigate the metabolomic variation during the albescent and re-greening stages, gas chromatography-mass spectrometry combined with multivariate analysis were applied to analyze the metabolite profiles in the different color stages during the development of 'Anji Baicha' leaves. The metabolite profiles of three albescent stages, including the yellow-green stage, the early albescent stage, and the late albescent stage, as well as the re-greening stage were distinguished using principal component analysis, revealing that the distinct developmental stages were likely responsible for the observed metabolic differences. Furthermore, a group classification and pairwise discrimination was revealed among the three albescent stages and re-greening stage by partial least squares discriminant analysis. A total of 65 differential metabolites were identified with a variable influence on projection greater than 1. The main differential metabolic pathways of the albescent stages compared with the re-greening stage included carbon fixation in photosynthetic organisms and the phenylpropanoid and flavonoid biosynthesis pathways. Compared with the re-greening stage, the carbohydrate and amino acid metabolic pathways were disturbed during the albescent stages. During the albescent stages, the sugar (fructofuranose), sugar derivative (glucose-1-phosphate) and epicatechin concentrations decreased, whereas the amino acid (mainly glycine, serine, tryptophan, citrulline, glutamine, proline, and valine) concentrations increased. These results reveal the changes in metabolic profiling that occur during the color changes associated with the development of the albino tea plant leaves.

No MeSH data available.


Related in: MedlinePlus