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Differential Metabolic Profiles during the Albescent Stages of 'Anji Baicha' (Camellia sinensis).

Li CF, Yao MZ, Ma CL, Ma JQ, Jin JQ, Chen L - PLoS ONE (2015)

Bottom Line: A total of 65 differential metabolites were identified with a variable influence on projection greater than 1.The main differential metabolic pathways of the albescent stages compared with the re-greening stage included carbon fixation in photosynthetic organisms and the phenylpropanoid and flavonoid biosynthesis pathways.During the albescent stages, the sugar (fructofuranose), sugar derivative (glucose-1-phosphate) and epicatechin concentrations decreased, whereas the amino acid (mainly glycine, serine, tryptophan, citrulline, glutamine, proline, and valine) concentrations increased.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou, China.

ABSTRACT
'Anji Baicha' is an albino tea cultivar with white shoots at low air temperature and green shoots at high air temperature in early spring. The metabolite contents in the shoots dynamically vary with the color changes and with shoot development. To investigate the metabolomic variation during the albescent and re-greening stages, gas chromatography-mass spectrometry combined with multivariate analysis were applied to analyze the metabolite profiles in the different color stages during the development of 'Anji Baicha' leaves. The metabolite profiles of three albescent stages, including the yellow-green stage, the early albescent stage, and the late albescent stage, as well as the re-greening stage were distinguished using principal component analysis, revealing that the distinct developmental stages were likely responsible for the observed metabolic differences. Furthermore, a group classification and pairwise discrimination was revealed among the three albescent stages and re-greening stage by partial least squares discriminant analysis. A total of 65 differential metabolites were identified with a variable influence on projection greater than 1. The main differential metabolic pathways of the albescent stages compared with the re-greening stage included carbon fixation in photosynthetic organisms and the phenylpropanoid and flavonoid biosynthesis pathways. Compared with the re-greening stage, the carbohydrate and amino acid metabolic pathways were disturbed during the albescent stages. During the albescent stages, the sugar (fructofuranose), sugar derivative (glucose-1-phosphate) and epicatechin concentrations decreased, whereas the amino acid (mainly glycine, serine, tryptophan, citrulline, glutamine, proline, and valine) concentrations increased. These results reveal the changes in metabolic profiling that occur during the color changes associated with the development of the albino tea plant leaves.

No MeSH data available.


Related in: MedlinePlus

PCA was used to compare the metabolic profiles of the tea plant at three albescent stages (YG, WI and WII) and the G stage.Score plots for principle components 1 and 2 showed high cohesion within groups and good separation among leaf groups from the three different albescent stages (YG, WI and WII) and the G stage. The sampling groups are color coded as follows: black = YG-stage leaves; blue = WI-stage leaves; green = WII-stage leaves; red = G-stage leaves; and purple = QC samples.
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pone.0139996.g002: PCA was used to compare the metabolic profiles of the tea plant at three albescent stages (YG, WI and WII) and the G stage.Score plots for principle components 1 and 2 showed high cohesion within groups and good separation among leaf groups from the three different albescent stages (YG, WI and WII) and the G stage. The sampling groups are color coded as follows: black = YG-stage leaves; blue = WI-stage leaves; green = WII-stage leaves; red = G-stage leaves; and purple = QC samples.

Mentions: The multi-dimensionality of complex data was reduced to principal components (PCs) that explain the maximal amount of variation within a sample. To gain an extensive comparison of the metabolic profiles of the three albescent stages (YG, WI and WII) and the G stage, a PCA (four components, R2X = 0.802, Q2 = 0.302) score plot (Fig 2) was constructed. Variation in the data set that was explained by one PC (PC1), two PCs (PC1 and PC2), three PCs (PC1, PC2 and PC3) and four PCs (PC1, PC2, PC3 and PC4) was 44.39%, 62.76%, 72.46% and 80.02%, respectively. QC samples were grouped together, indicating that the QC samples had similar metabolic profiles and that our entire analysis had satisfactory stability and repeatability. A large variation in water content between groups can cause an over-representation of the sample groups with lower water content due to the use of fresh mass to prepare the QC. This variation likely led to the QC sample being closer to WI and WII than the other groups.


Differential Metabolic Profiles during the Albescent Stages of 'Anji Baicha' (Camellia sinensis).

Li CF, Yao MZ, Ma CL, Ma JQ, Jin JQ, Chen L - PLoS ONE (2015)

PCA was used to compare the metabolic profiles of the tea plant at three albescent stages (YG, WI and WII) and the G stage.Score plots for principle components 1 and 2 showed high cohesion within groups and good separation among leaf groups from the three different albescent stages (YG, WI and WII) and the G stage. The sampling groups are color coded as follows: black = YG-stage leaves; blue = WI-stage leaves; green = WII-stage leaves; red = G-stage leaves; and purple = QC samples.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4622044&req=5

pone.0139996.g002: PCA was used to compare the metabolic profiles of the tea plant at three albescent stages (YG, WI and WII) and the G stage.Score plots for principle components 1 and 2 showed high cohesion within groups and good separation among leaf groups from the three different albescent stages (YG, WI and WII) and the G stage. The sampling groups are color coded as follows: black = YG-stage leaves; blue = WI-stage leaves; green = WII-stage leaves; red = G-stage leaves; and purple = QC samples.
Mentions: The multi-dimensionality of complex data was reduced to principal components (PCs) that explain the maximal amount of variation within a sample. To gain an extensive comparison of the metabolic profiles of the three albescent stages (YG, WI and WII) and the G stage, a PCA (four components, R2X = 0.802, Q2 = 0.302) score plot (Fig 2) was constructed. Variation in the data set that was explained by one PC (PC1), two PCs (PC1 and PC2), three PCs (PC1, PC2 and PC3) and four PCs (PC1, PC2, PC3 and PC4) was 44.39%, 62.76%, 72.46% and 80.02%, respectively. QC samples were grouped together, indicating that the QC samples had similar metabolic profiles and that our entire analysis had satisfactory stability and repeatability. A large variation in water content between groups can cause an over-representation of the sample groups with lower water content due to the use of fresh mass to prepare the QC. This variation likely led to the QC sample being closer to WI and WII than the other groups.

Bottom Line: A total of 65 differential metabolites were identified with a variable influence on projection greater than 1.The main differential metabolic pathways of the albescent stages compared with the re-greening stage included carbon fixation in photosynthetic organisms and the phenylpropanoid and flavonoid biosynthesis pathways.During the albescent stages, the sugar (fructofuranose), sugar derivative (glucose-1-phosphate) and epicatechin concentrations decreased, whereas the amino acid (mainly glycine, serine, tryptophan, citrulline, glutamine, proline, and valine) concentrations increased.

View Article: PubMed Central - PubMed

Affiliation: Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Tea Research Institute of the Chinese Academy of Agricultural Sciences, Hangzhou, China.

ABSTRACT
'Anji Baicha' is an albino tea cultivar with white shoots at low air temperature and green shoots at high air temperature in early spring. The metabolite contents in the shoots dynamically vary with the color changes and with shoot development. To investigate the metabolomic variation during the albescent and re-greening stages, gas chromatography-mass spectrometry combined with multivariate analysis were applied to analyze the metabolite profiles in the different color stages during the development of 'Anji Baicha' leaves. The metabolite profiles of three albescent stages, including the yellow-green stage, the early albescent stage, and the late albescent stage, as well as the re-greening stage were distinguished using principal component analysis, revealing that the distinct developmental stages were likely responsible for the observed metabolic differences. Furthermore, a group classification and pairwise discrimination was revealed among the three albescent stages and re-greening stage by partial least squares discriminant analysis. A total of 65 differential metabolites were identified with a variable influence on projection greater than 1. The main differential metabolic pathways of the albescent stages compared with the re-greening stage included carbon fixation in photosynthetic organisms and the phenylpropanoid and flavonoid biosynthesis pathways. Compared with the re-greening stage, the carbohydrate and amino acid metabolic pathways were disturbed during the albescent stages. During the albescent stages, the sugar (fructofuranose), sugar derivative (glucose-1-phosphate) and epicatechin concentrations decreased, whereas the amino acid (mainly glycine, serine, tryptophan, citrulline, glutamine, proline, and valine) concentrations increased. These results reveal the changes in metabolic profiling that occur during the color changes associated with the development of the albino tea plant leaves.

No MeSH data available.


Related in: MedlinePlus