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Butyrylated starch affects colorectal cancer markers beneficially and dose-dependently in genotoxin-treated rats.

Toden S, Lockett TJ, Topping DL, Scherer BL, Watson EJ, Southwood JG, Clarke JM - Cancer Biol. Ther. (2014)

Bottom Line: Large bowel total SCFA, acetate and butyrate pools and hepatic portal venous plasma total SCFA, acetate and butyrate concentrations were higher with greater HAMSB intake.Distal colonic epithelial apoptotic index and colonic mucus thickness increased, while DNA single strand breaks decreased dose-dependently with greater HAMSB intake.These data suggest that increasing large bowel butyrate may reduce the risk of CRC in a dose dependent manner by enhancing apoptotic surveillance in the colonic epithelium for damaged cells without promoting the risk of tumorigenesis through increased cell proliferation.

View Article: PubMed Central - PubMed

Affiliation: a Preventative Health National Research Flagship ; Adelaide , Australia.

ABSTRACT
Population studies suggest that greater dietary fiber intake may lower colorectal cancer (CRC) risk, possibly through the colonic bacterial fermentative production of butyrate. Butyrylated starch delivers butyrate to the colon of humans with potential to reduce CRC risk but high doses may exacerbate risk through promoting epithelial proliferation. Here we report the effects of increasing dietary butyrylated high amylose maize starch (HAMSB) on azoxymethane (AOM) induced distal colonic DNA damage, cell proliferation, mucus layer thickness and apoptosis in rats. Five groups of 15 rats were fed AIN-93G based diets containing 0-40% HAMSB for 4 weeks then injected with (AOM) and killed 6 hours later. Large bowel total SCFA, acetate and butyrate pools and hepatic portal venous plasma total SCFA, acetate and butyrate concentrations were higher with greater HAMSB intake. Distal colonic epithelial apoptotic index and colonic mucus thickness increased, while DNA single strand breaks decreased dose-dependently with greater HAMSB intake. Colonocyte proliferation rates were unaffected by diet. These data suggest that increasing large bowel butyrate may reduce the risk of CRC in a dose dependent manner by enhancing apoptotic surveillance in the colonic epithelium for damaged cells without promoting the risk of tumorigenesis through increased cell proliferation.

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Related in: MedlinePlus

Short chain fatty acid levels in (A) hepatic portal vein plasma concentrations (μmol/L) (B) cecal pools (μmoles) (C) cecal concentrations (μmol/L) (D) faecal pools (μmoles). Columns within a graph not sharing the same letter are significantly different P< 0.05, mean±SEM , n = 15/group).
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f0001: Short chain fatty acid levels in (A) hepatic portal vein plasma concentrations (μmol/L) (B) cecal pools (μmoles) (C) cecal concentrations (μmol/L) (D) faecal pools (μmoles). Columns within a graph not sharing the same letter are significantly different P< 0.05, mean±SEM , n = 15/group).

Mentions: Hepatic portal plasma total SCFA, acetate and butyrate concentrations increased with higher intakes of dietary HAMSB (Fig. 1A). However, there was no difference in hepatic portal vein plasma propionate concentrations.Figure 1.


Butyrylated starch affects colorectal cancer markers beneficially and dose-dependently in genotoxin-treated rats.

Toden S, Lockett TJ, Topping DL, Scherer BL, Watson EJ, Southwood JG, Clarke JM - Cancer Biol. Ther. (2014)

Short chain fatty acid levels in (A) hepatic portal vein plasma concentrations (μmol/L) (B) cecal pools (μmoles) (C) cecal concentrations (μmol/L) (D) faecal pools (μmoles). Columns within a graph not sharing the same letter are significantly different P< 0.05, mean±SEM , n = 15/group).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4622003&req=5

f0001: Short chain fatty acid levels in (A) hepatic portal vein plasma concentrations (μmol/L) (B) cecal pools (μmoles) (C) cecal concentrations (μmol/L) (D) faecal pools (μmoles). Columns within a graph not sharing the same letter are significantly different P< 0.05, mean±SEM , n = 15/group).
Mentions: Hepatic portal plasma total SCFA, acetate and butyrate concentrations increased with higher intakes of dietary HAMSB (Fig. 1A). However, there was no difference in hepatic portal vein plasma propionate concentrations.Figure 1.

Bottom Line: Large bowel total SCFA, acetate and butyrate pools and hepatic portal venous plasma total SCFA, acetate and butyrate concentrations were higher with greater HAMSB intake.Distal colonic epithelial apoptotic index and colonic mucus thickness increased, while DNA single strand breaks decreased dose-dependently with greater HAMSB intake.These data suggest that increasing large bowel butyrate may reduce the risk of CRC in a dose dependent manner by enhancing apoptotic surveillance in the colonic epithelium for damaged cells without promoting the risk of tumorigenesis through increased cell proliferation.

View Article: PubMed Central - PubMed

Affiliation: a Preventative Health National Research Flagship ; Adelaide , Australia.

ABSTRACT
Population studies suggest that greater dietary fiber intake may lower colorectal cancer (CRC) risk, possibly through the colonic bacterial fermentative production of butyrate. Butyrylated starch delivers butyrate to the colon of humans with potential to reduce CRC risk but high doses may exacerbate risk through promoting epithelial proliferation. Here we report the effects of increasing dietary butyrylated high amylose maize starch (HAMSB) on azoxymethane (AOM) induced distal colonic DNA damage, cell proliferation, mucus layer thickness and apoptosis in rats. Five groups of 15 rats were fed AIN-93G based diets containing 0-40% HAMSB for 4 weeks then injected with (AOM) and killed 6 hours later. Large bowel total SCFA, acetate and butyrate pools and hepatic portal venous plasma total SCFA, acetate and butyrate concentrations were higher with greater HAMSB intake. Distal colonic epithelial apoptotic index and colonic mucus thickness increased, while DNA single strand breaks decreased dose-dependently with greater HAMSB intake. Colonocyte proliferation rates were unaffected by diet. These data suggest that increasing large bowel butyrate may reduce the risk of CRC in a dose dependent manner by enhancing apoptotic surveillance in the colonic epithelium for damaged cells without promoting the risk of tumorigenesis through increased cell proliferation.

Show MeSH
Related in: MedlinePlus