Limits...
Analysis of the reaction of subcutaneous tissues in rats and the antimicrobial activity of calcium hydroxide paste used in association with different substances.

Midena RZ, Garcia RB, Cavenago BC, Marciano MA, Minotti PG, Ordinola-Zapata R, Weckwerth PH, Andrade FB, Duarte MA - J Appl Oral Sci (2015)

Bottom Line: The results showed that 0.4% chlorhexidine induced a higher inflammatory response than the other groups.The Casearia sylvestris Sw extract showed satisfactory results in relation to the intensity of the inflammatory response.Antimicrobial components such as chlorhexidine or Casearia sylvestris Sw did not improve the antimicrobial activity against E. faecalis in comparison to the calcium hydroxide+PG treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.

ABSTRACT
The aim of this study was to evaluate the subcutaneous tissue response in rats and the antimicrobial activity of intracanal calcium hydroxide dressings mixed with different substances against E. faecalis. Fifty four rats were divided into three experimental groups according to the vehicle in the calcium hydroxide treatment: 0.4% chlorohexidine in propylene glycol (PG),Casearia sylvestris Sw in PG and calcium hydroxide+PG (control group). The pastes were placed into polyethylene tubes and implanted into the subcutaneous tissue. After 7, 14 and 30 days, the samples were processed and histologically evaluated (hematoxylin and eosin). The tissue surface in contact with the material was analyzed, and the quantitative analysis determined the volume density occupied by the inflammatory infiltrate (giant cells, polymorphonuclear cells and mononuclear cells), fibroblasts, collagen fibers and blood vessels. For the antimicrobial analysis, 20 dentin blocks infected with E. faecalis were treated with calcium hydroxide pastes in different vehicles; 0.4% chlorhexidine in PG, PG, extract fromCasearia sylvestris Sw in PG and a positive control (infection and without medication) for 7 days. The efficiency of the pastes was evaluated by the live/dead technique and confocal microscopy. The results showed that 0.4% chlorhexidine induced a higher inflammatory response than the other groups. The Casearia sylvestris Sw extract showed satisfactory results in relation to the intensity of the inflammatory response. In the microbiological test, there were no statistical differences between the evaluated intracanal dressings and the percentage of bacterial viability was between 33 and 42%. The control group showed an 86% viability. Antimicrobial components such as chlorhexidine or Casearia sylvestris Sw did not improve the antimicrobial activity against E. faecalis in comparison to the calcium hydroxide+PG treatment. In addition, the incorporation of chlorhexidine in the calcium hydroxide paste promoted the highest inflammatory response.

No MeSH data available.


Related in: MedlinePlus

Week one subcutaneous tissue response of the evaluated intracanal dressings (7 days). Chlorhexidine 0.4%+calcium hydroxide (A); Casearia sylvestris Sw+calcium hydroxide (B); Propylene glycol+calcium hydroxide (C). Fibrocellular tissue (*), macrophage infiltration (arrows) and material remnants (●) can be observed. At 14 days, a decrease in the inflammatory infiltrate (arrows) and a discrete increase in fibrocellular tissue (*) in comparison to the 7 day period were observed. Chlorhexidine 0.4%+calcium hydroxide (D); Casearia sylvestris Sw+calcium hydroxide (E); Propylene glycol+calcium hydroxide (F). After 30 days, organized subcutaneous tissue is evident. Chlorhexidine 0.4%+calcium hydroxide (G) presenting giant cells (arrow). Fibrocellular tissue (*) is present in Casearia sylvestris Sw+calcium hydroxide (H) and propylene glycol+calcium hydroxide (I)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4621947&req=5

f01: Week one subcutaneous tissue response of the evaluated intracanal dressings (7 days). Chlorhexidine 0.4%+calcium hydroxide (A); Casearia sylvestris Sw+calcium hydroxide (B); Propylene glycol+calcium hydroxide (C). Fibrocellular tissue (*), macrophage infiltration (arrows) and material remnants (●) can be observed. At 14 days, a decrease in the inflammatory infiltrate (arrows) and a discrete increase in fibrocellular tissue (*) in comparison to the 7 day period were observed. Chlorhexidine 0.4%+calcium hydroxide (D); Casearia sylvestris Sw+calcium hydroxide (E); Propylene glycol+calcium hydroxide (F). After 30 days, organized subcutaneous tissue is evident. Chlorhexidine 0.4%+calcium hydroxide (G) presenting giant cells (arrow). Fibrocellular tissue (*) is present in Casearia sylvestris Sw+calcium hydroxide (H) and propylene glycol+calcium hydroxide (I)

Mentions: The values and statistical comparisons concerning the intensity of the inflammatory cells, collagen fibers, fibroblasts and blood vessels between the groups in each experimental period and between periods for each group are presented in Table 1 and Table 2. At the initial periods, it was possible to observe amorphous tissue in the contact region with the material, suggestive of coagulation necrosis, probably due to the alkaline action of the calcium hydroxide. Overall, the lowest values were found at the 30 day period for all pastes tested. In the 7 day period there were no statistically significant differences (P>0.05) among the tested pastes (P>0.05), however, during the periods of 14 to 30 days, statistically significant differences were found (P<0.05). A representative picture of the inflammatory reaction is shown in Figure 1.


Analysis of the reaction of subcutaneous tissues in rats and the antimicrobial activity of calcium hydroxide paste used in association with different substances.

Midena RZ, Garcia RB, Cavenago BC, Marciano MA, Minotti PG, Ordinola-Zapata R, Weckwerth PH, Andrade FB, Duarte MA - J Appl Oral Sci (2015)

Week one subcutaneous tissue response of the evaluated intracanal dressings (7 days). Chlorhexidine 0.4%+calcium hydroxide (A); Casearia sylvestris Sw+calcium hydroxide (B); Propylene glycol+calcium hydroxide (C). Fibrocellular tissue (*), macrophage infiltration (arrows) and material remnants (●) can be observed. At 14 days, a decrease in the inflammatory infiltrate (arrows) and a discrete increase in fibrocellular tissue (*) in comparison to the 7 day period were observed. Chlorhexidine 0.4%+calcium hydroxide (D); Casearia sylvestris Sw+calcium hydroxide (E); Propylene glycol+calcium hydroxide (F). After 30 days, organized subcutaneous tissue is evident. Chlorhexidine 0.4%+calcium hydroxide (G) presenting giant cells (arrow). Fibrocellular tissue (*) is present in Casearia sylvestris Sw+calcium hydroxide (H) and propylene glycol+calcium hydroxide (I)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4621947&req=5

f01: Week one subcutaneous tissue response of the evaluated intracanal dressings (7 days). Chlorhexidine 0.4%+calcium hydroxide (A); Casearia sylvestris Sw+calcium hydroxide (B); Propylene glycol+calcium hydroxide (C). Fibrocellular tissue (*), macrophage infiltration (arrows) and material remnants (●) can be observed. At 14 days, a decrease in the inflammatory infiltrate (arrows) and a discrete increase in fibrocellular tissue (*) in comparison to the 7 day period were observed. Chlorhexidine 0.4%+calcium hydroxide (D); Casearia sylvestris Sw+calcium hydroxide (E); Propylene glycol+calcium hydroxide (F). After 30 days, organized subcutaneous tissue is evident. Chlorhexidine 0.4%+calcium hydroxide (G) presenting giant cells (arrow). Fibrocellular tissue (*) is present in Casearia sylvestris Sw+calcium hydroxide (H) and propylene glycol+calcium hydroxide (I)
Mentions: The values and statistical comparisons concerning the intensity of the inflammatory cells, collagen fibers, fibroblasts and blood vessels between the groups in each experimental period and between periods for each group are presented in Table 1 and Table 2. At the initial periods, it was possible to observe amorphous tissue in the contact region with the material, suggestive of coagulation necrosis, probably due to the alkaline action of the calcium hydroxide. Overall, the lowest values were found at the 30 day period for all pastes tested. In the 7 day period there were no statistically significant differences (P>0.05) among the tested pastes (P>0.05), however, during the periods of 14 to 30 days, statistically significant differences were found (P<0.05). A representative picture of the inflammatory reaction is shown in Figure 1.

Bottom Line: The results showed that 0.4% chlorhexidine induced a higher inflammatory response than the other groups.The Casearia sylvestris Sw extract showed satisfactory results in relation to the intensity of the inflammatory response.Antimicrobial components such as chlorhexidine or Casearia sylvestris Sw did not improve the antimicrobial activity against E. faecalis in comparison to the calcium hydroxide+PG treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of Dentistry, Endodontics and Dental Materials, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Brazil.

ABSTRACT
The aim of this study was to evaluate the subcutaneous tissue response in rats and the antimicrobial activity of intracanal calcium hydroxide dressings mixed with different substances against E. faecalis. Fifty four rats were divided into three experimental groups according to the vehicle in the calcium hydroxide treatment: 0.4% chlorohexidine in propylene glycol (PG),Casearia sylvestris Sw in PG and calcium hydroxide+PG (control group). The pastes were placed into polyethylene tubes and implanted into the subcutaneous tissue. After 7, 14 and 30 days, the samples were processed and histologically evaluated (hematoxylin and eosin). The tissue surface in contact with the material was analyzed, and the quantitative analysis determined the volume density occupied by the inflammatory infiltrate (giant cells, polymorphonuclear cells and mononuclear cells), fibroblasts, collagen fibers and blood vessels. For the antimicrobial analysis, 20 dentin blocks infected with E. faecalis were treated with calcium hydroxide pastes in different vehicles; 0.4% chlorhexidine in PG, PG, extract fromCasearia sylvestris Sw in PG and a positive control (infection and without medication) for 7 days. The efficiency of the pastes was evaluated by the live/dead technique and confocal microscopy. The results showed that 0.4% chlorhexidine induced a higher inflammatory response than the other groups. The Casearia sylvestris Sw extract showed satisfactory results in relation to the intensity of the inflammatory response. In the microbiological test, there were no statistical differences between the evaluated intracanal dressings and the percentage of bacterial viability was between 33 and 42%. The control group showed an 86% viability. Antimicrobial components such as chlorhexidine or Casearia sylvestris Sw did not improve the antimicrobial activity against E. faecalis in comparison to the calcium hydroxide+PG treatment. In addition, the incorporation of chlorhexidine in the calcium hydroxide paste promoted the highest inflammatory response.

No MeSH data available.


Related in: MedlinePlus