Limits...
Microbial contamination and disinfection methods of pacifiers.

Nelson-Filho P, Louvain MC, Macari S, Lucisano MP, Silva RA, Queiroz AM, Gaton-Hernández P, Silva LA - J Appl Oral Sci (2015)

Bottom Line: The results were analyzed statistically by Friedman's non-parametric test (a=0.05).Results The 0.12% chlorhexidine spray was statistically similar to the boiling water (p>0.05) and more effective than the Brushtox®spray and control (p<0.05).The analysis of SEM showed the formation of a cariogenic biofilm in all groups with positive culture.Conclusions Pacifiers become contaminated by MS after their use by children and should be disinfected routinely.Spraying with a 0.12% chlorhexidine solution and immersion in boiling water promoted better disinfection of the pacifiers compared with a commercial antiseptic toothbrush cleanser (Brushtox®).

View Article: PubMed Central - PubMed

Affiliation: Departamento de Odontopediatria, Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.

ABSTRACT
Objectives To evaluate the microbial contamination of pacifiers by Mutans Streptococci(MS) and the efficacy of different methods for their disinfection.Methods Twenty-eight children were assigned to a 4-stage changeover system with a 1-week interval. In each stage, children received a new pacifier and the parents were instructed to maintain their normal habits for 1 week. After this time, the pacifiers were subjected to the following 4 disinfection methods: spraying with 0.12% chlorhexidine solution, Brushtox® or sterile tap water, and immersion in boiling tap water for 15 minutes. Microbiological culture for MS and Scanning Electron Microscopy (SEM) were performed. The results were analyzed statistically by Friedman's non-parametric test (a=0.05).Results The 0.12% chlorhexidine spray was statistically similar to the boiling water (p>0.05) and more effective than the Brushtox®spray and control (p<0.05). The analysis of SEM showed the formation of a cariogenic biofilm in all groups with positive culture.Conclusions Pacifiers become contaminated by MS after their use by children and should be disinfected routinely. Spraying with a 0.12% chlorhexidine solution and immersion in boiling water promoted better disinfection of the pacifiers compared with a commercial antiseptic toothbrush cleanser (Brushtox®).

No MeSH data available.


Related in: MedlinePlus

Sterile tap water spray - Colonies/biofilms of Mutans streptococci after microbial culture (A) and scanning electron microscopy micrograph (B)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4621945&req=5

f04: Sterile tap water spray - Colonies/biofilms of Mutans streptococci after microbial culture (A) and scanning electron microscopy micrograph (B)

Mentions: Colonies/biofilms of MS were present in 5 (17.86%) of the 28 pacifiers sprayed with chlorhexidine, whose score was of 2 (3.58%), while the others scored 1. The number of colonies/biofilms ranged from 1 to 50. When Brushtox® was used, colonies/biofilms of MS were present in 18 (64.29%) of the 28 pacifiers, with scores from 1 to 3. The number of colonies/biofilms ranged from 1 to more than 50. After immersion in boiling water for 15 minutes, 6 (21.42%) of the 28 pacifiers have showed MS colonization of score 1, with the number of colonies/biofilms ranging from 1 to 20. The total of 28 (100.0%) pacifiers sprayed with sterile tap water ended up being contaminated with MS, ranging from 1 to more than 50 colonies/biofilms, with scores from 1 to 3. Seventeen (60.71%) pacifiers scored 1, 7 (25%) scored 2, and 4 (14.29%) scored 3. Figures 1A-4A show representative pacifiers of the 4 disinfection methods, respectively. There was no bacterial contamination in the 3 non-used pacifiers after incubation.


Microbial contamination and disinfection methods of pacifiers.

Nelson-Filho P, Louvain MC, Macari S, Lucisano MP, Silva RA, Queiroz AM, Gaton-Hernández P, Silva LA - J Appl Oral Sci (2015)

Sterile tap water spray - Colonies/biofilms of Mutans streptococci after microbial culture (A) and scanning electron microscopy micrograph (B)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4621945&req=5

f04: Sterile tap water spray - Colonies/biofilms of Mutans streptococci after microbial culture (A) and scanning electron microscopy micrograph (B)
Mentions: Colonies/biofilms of MS were present in 5 (17.86%) of the 28 pacifiers sprayed with chlorhexidine, whose score was of 2 (3.58%), while the others scored 1. The number of colonies/biofilms ranged from 1 to 50. When Brushtox® was used, colonies/biofilms of MS were present in 18 (64.29%) of the 28 pacifiers, with scores from 1 to 3. The number of colonies/biofilms ranged from 1 to more than 50. After immersion in boiling water for 15 minutes, 6 (21.42%) of the 28 pacifiers have showed MS colonization of score 1, with the number of colonies/biofilms ranging from 1 to 20. The total of 28 (100.0%) pacifiers sprayed with sterile tap water ended up being contaminated with MS, ranging from 1 to more than 50 colonies/biofilms, with scores from 1 to 3. Seventeen (60.71%) pacifiers scored 1, 7 (25%) scored 2, and 4 (14.29%) scored 3. Figures 1A-4A show representative pacifiers of the 4 disinfection methods, respectively. There was no bacterial contamination in the 3 non-used pacifiers after incubation.

Bottom Line: The results were analyzed statistically by Friedman's non-parametric test (a=0.05).Results The 0.12% chlorhexidine spray was statistically similar to the boiling water (p>0.05) and more effective than the Brushtox®spray and control (p<0.05).The analysis of SEM showed the formation of a cariogenic biofilm in all groups with positive culture.Conclusions Pacifiers become contaminated by MS after their use by children and should be disinfected routinely.Spraying with a 0.12% chlorhexidine solution and immersion in boiling water promoted better disinfection of the pacifiers compared with a commercial antiseptic toothbrush cleanser (Brushtox®).

View Article: PubMed Central - PubMed

Affiliation: Departamento de Odontopediatria, Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.

ABSTRACT
Objectives To evaluate the microbial contamination of pacifiers by Mutans Streptococci(MS) and the efficacy of different methods for their disinfection.Methods Twenty-eight children were assigned to a 4-stage changeover system with a 1-week interval. In each stage, children received a new pacifier and the parents were instructed to maintain their normal habits for 1 week. After this time, the pacifiers were subjected to the following 4 disinfection methods: spraying with 0.12% chlorhexidine solution, Brushtox® or sterile tap water, and immersion in boiling tap water for 15 minutes. Microbiological culture for MS and Scanning Electron Microscopy (SEM) were performed. The results were analyzed statistically by Friedman's non-parametric test (a=0.05).Results The 0.12% chlorhexidine spray was statistically similar to the boiling water (p>0.05) and more effective than the Brushtox®spray and control (p<0.05). The analysis of SEM showed the formation of a cariogenic biofilm in all groups with positive culture.Conclusions Pacifiers become contaminated by MS after their use by children and should be disinfected routinely. Spraying with a 0.12% chlorhexidine solution and immersion in boiling water promoted better disinfection of the pacifiers compared with a commercial antiseptic toothbrush cleanser (Brushtox®).

No MeSH data available.


Related in: MedlinePlus