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Effects on gastric mucosa induced by dental bleaching - an experimental study with 6% hydrogen peroxide in rats.

Paula AB, Dias MI, Ferreira MM, Carrilho T, Marto CM, Casalta J, Cabrita AS, Carrilho E - J Appl Oral Sci (2015)

Bottom Line: There was a decrease in body weight in animals of groups handled during the study period, which was most pronounced in IV and VA groups.Changes in spleen weight relative to body weight revealed no statistically significant changes.An analysis of the frequency was performed on the results of macroscopic observation of the gastric mucosa.Results The gastric mucosa revealed lesions in all manipulated groups, being more frequent in groups III and IV.

View Article: PubMed Central - PubMed

Affiliation: Escola de Medicina, Universidade de Coimbra, Coimbra, Portugal.

ABSTRACT
The value of aesthetic dentistry has precipitated several developments in the investigation of dental materials related to this field. The free marketing of these products is a problem and it is subject to various interpretations regarding its legality. There are several techniques for tooth whitening, the most used one being the external bleaching. It is the later version of such technique that poses the greatest danger of ingesting the product. The present study analysed the systemic effect of these products when they are swallowed.Objective This experimental study aimed to observe the effects of a tooth whitening product, whose active agent is 6% hydrogen peroxide, on the gastric mucosa of healthy and non-tumour gastric pathology animals.Material and Methods Fifty Wistar-Han rats were used and then distributed into 5 groups, one for control and four test groups in which the bleaching product was administered in animals with and without non-tumour gastric pathology (induced by the administration of 1 sample of 50% ethanol and 5% of drinking water during 6 days) at different times of study by gavage. There was a decrease in body weight in animals of groups handled during the study period, which was most pronounced in IV and VA groups. Changes in spleen weight relative to body weight revealed no statistically significant changes. An analysis of the frequency was performed on the results of macroscopic observation of the gastric mucosa.Results The gastric mucosa revealed lesions in all manipulated groups, being more frequent in groups III and IV. It appears that there is a synergism when using hydrogen peroxide and 50% ethanol in the same group.Conclusion Therefore, it seems that there are some signs of toxicity 3 to 4 days after administration of 6% hydrogen peroxide. The prescription of these therapies must be controlled by the clinician and the risks must be minimized.

No MeSH data available.


Related in: MedlinePlus

Experimental procedure performed in the various groups. (Q - quarantine, P - hydrogen peroxide administration; G - non-tumoral gastric pathology induced)
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f01: Experimental procedure performed in the various groups. (Q - quarantine, P - hydrogen peroxide administration; G - non-tumoral gastric pathology induced)

Mentions: For this study, we used 50 rats of the strain Wistar-Han of 8 weeks of age, with about 250 grams in weight. In week 1, all animals were quarantined. In periods II and III, which took place in the 2nd and 3rd weeks, the animals were subjected to different test protocols, and divided into 5 groups with 10 animals each. The group I was the negative control, in which the animals were not manipulated, while the groups II and V were the positive control groups in which the animals were subjected to the administration of a bleaching agent (group II) and to an non-tumour gastric pathology induction protocol by administering a chemical compound (group V). These groups were divided into two subgroups to control all other test groups. In group III, which is the test group that expresses the goal of the study, the animals were subjected to a non-tumour gastric pathology induction protocol by administering a chemical compound in the 2nd week and the administration of a bleaching agent in the 3rd week. In the group IV the administration were exactly the opposite of the previous group. All products were administered to the animals by gavage. Figure 1 summarizes the experimental procedure performed in the various groups.


Effects on gastric mucosa induced by dental bleaching - an experimental study with 6% hydrogen peroxide in rats.

Paula AB, Dias MI, Ferreira MM, Carrilho T, Marto CM, Casalta J, Cabrita AS, Carrilho E - J Appl Oral Sci (2015)

Experimental procedure performed in the various groups. (Q - quarantine, P - hydrogen peroxide administration; G - non-tumoral gastric pathology induced)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4621943&req=5

f01: Experimental procedure performed in the various groups. (Q - quarantine, P - hydrogen peroxide administration; G - non-tumoral gastric pathology induced)
Mentions: For this study, we used 50 rats of the strain Wistar-Han of 8 weeks of age, with about 250 grams in weight. In week 1, all animals were quarantined. In periods II and III, which took place in the 2nd and 3rd weeks, the animals were subjected to different test protocols, and divided into 5 groups with 10 animals each. The group I was the negative control, in which the animals were not manipulated, while the groups II and V were the positive control groups in which the animals were subjected to the administration of a bleaching agent (group II) and to an non-tumour gastric pathology induction protocol by administering a chemical compound (group V). These groups were divided into two subgroups to control all other test groups. In group III, which is the test group that expresses the goal of the study, the animals were subjected to a non-tumour gastric pathology induction protocol by administering a chemical compound in the 2nd week and the administration of a bleaching agent in the 3rd week. In the group IV the administration were exactly the opposite of the previous group. All products were administered to the animals by gavage. Figure 1 summarizes the experimental procedure performed in the various groups.

Bottom Line: There was a decrease in body weight in animals of groups handled during the study period, which was most pronounced in IV and VA groups.Changes in spleen weight relative to body weight revealed no statistically significant changes.An analysis of the frequency was performed on the results of macroscopic observation of the gastric mucosa.Results The gastric mucosa revealed lesions in all manipulated groups, being more frequent in groups III and IV.

View Article: PubMed Central - PubMed

Affiliation: Escola de Medicina, Universidade de Coimbra, Coimbra, Portugal.

ABSTRACT
The value of aesthetic dentistry has precipitated several developments in the investigation of dental materials related to this field. The free marketing of these products is a problem and it is subject to various interpretations regarding its legality. There are several techniques for tooth whitening, the most used one being the external bleaching. It is the later version of such technique that poses the greatest danger of ingesting the product. The present study analysed the systemic effect of these products when they are swallowed.Objective This experimental study aimed to observe the effects of a tooth whitening product, whose active agent is 6% hydrogen peroxide, on the gastric mucosa of healthy and non-tumour gastric pathology animals.Material and Methods Fifty Wistar-Han rats were used and then distributed into 5 groups, one for control and four test groups in which the bleaching product was administered in animals with and without non-tumour gastric pathology (induced by the administration of 1 sample of 50% ethanol and 5% of drinking water during 6 days) at different times of study by gavage. There was a decrease in body weight in animals of groups handled during the study period, which was most pronounced in IV and VA groups. Changes in spleen weight relative to body weight revealed no statistically significant changes. An analysis of the frequency was performed on the results of macroscopic observation of the gastric mucosa.Results The gastric mucosa revealed lesions in all manipulated groups, being more frequent in groups III and IV. It appears that there is a synergism when using hydrogen peroxide and 50% ethanol in the same group.Conclusion Therefore, it seems that there are some signs of toxicity 3 to 4 days after administration of 6% hydrogen peroxide. The prescription of these therapies must be controlled by the clinician and the risks must be minimized.

No MeSH data available.


Related in: MedlinePlus