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Altered β-catenin expression in oral mucosal dysplasia: a comparative study.

Silva BS, Castro CA, VON Zeidler SL, Sousa SC, Batista AC, Yamamoto-Silva FP - J Appl Oral Sci (2015)

Bottom Line: The Chi-square test and the Fisher's exact test were used to verify possible differences in the β-catenin expression among the OL groups.A p-value of <0.05 was considered statistically significant.Results Membranous expression of β-catenin in parabasal and basal layers was gradually lost in the higher degrees of epithelial dysplasia.In normal oral mucosa, β-catenin was detected only in the cytoplasmic membrane.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ciências Estomatológicas, Faculdade de Odontologia, Universidade Federal de Goiás, Goiânia, GO, Brazil.

ABSTRACT
Objective The current study aimed to investigate the β-catenin expression in oral leukoplakia (OL) with different degrees of epithelial dysplasia and normal oral mucosa.Material and Methods Formalin-fixed, paraffin-embedded tissue samples of 39 OL (mild dysplasia n=19, moderate dysplasia n=13, and severe dysplasia n=7), and 10 normal oral mucosa (control group) were submitted to immunohistochemical reactions to anti-β-catenin primary antibody. A qualitative β-catenin analysis was performed based on the percentage of positive cells. The cellular location and the epithelial layer were also considered. The Chi-square test and the Fisher's exact test were used to verify possible differences in the β-catenin expression among the OL groups. A p-value of <0.05 was considered statistically significant.Results Membranous expression of β-catenin in parabasal and basal layers was gradually lost in the higher degrees of epithelial dysplasia. In normal oral mucosa, β-catenin was detected only in the cytoplasmic membrane. However, a significant increase in cytoplasmic β-catenin could be observed between mild and moderate dysplasia (Fisher Exact test - p<0.001) and between mild and severe dysplasia (p<0.001).Conclusions The β-catenin cytoplasmic expression observed in this study may represent the initial stage of modifications in the E-cadherin-catenin complex, along with morphological cellular changes.

No MeSH data available.


Related in: MedlinePlus

β-catenin immunohistochemical expression in oral leukoplakia (OL) (mild, moderate and severe dysplasia) and normal oral mucosa. (A) β-catenin cytoplasmic membrane staining, in all three layers of mild dysplasia (x100), and (B) in the cytoplasm of basal and parabasal cells. (C, D) Illustrations of the β-catenin cytoplasmic membrane (CM) expression in the upper layers of moderate dysplasia, showing a decrease in cytoplasmic membrane expression, with more evident cytoplasmic localization in basal and parabasal cells (arrows head) (x100 - x400). (E, F) Weak membranous expression of β-catenin in parabasal and basal layers of severe dysplasia, with a significant increase in its expression, in the cytoplasm (arrows head) of cells of basaloid appearance (x100 - x400). (G, H) β-catenin in normal oral mucosa (x100 - x400)
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f02: β-catenin immunohistochemical expression in oral leukoplakia (OL) (mild, moderate and severe dysplasia) and normal oral mucosa. (A) β-catenin cytoplasmic membrane staining, in all three layers of mild dysplasia (x100), and (B) in the cytoplasm of basal and parabasal cells. (C, D) Illustrations of the β-catenin cytoplasmic membrane (CM) expression in the upper layers of moderate dysplasia, showing a decrease in cytoplasmic membrane expression, with more evident cytoplasmic localization in basal and parabasal cells (arrows head) (x100 - x400). (E, F) Weak membranous expression of β-catenin in parabasal and basal layers of severe dysplasia, with a significant increase in its expression, in the cytoplasm (arrows head) of cells of basaloid appearance (x100 - x400). (G, H) β-catenin in normal oral mucosa (x100 - x400)

Mentions: In general, β-catenin immunohistochemical staining was observed in the cytoplasmic membrane in all OL dysplastic groups (n=39; mild, moderate and severe dysplasia) (Figure 2). In mild, moderate and severe dysplasia, β-catenin staining was also detected in the cytoplasm in 51.2% (n=20) of OL cases (Figure 2, A-F). In addition, membranous expression of β-catenin in parabasal and basal layers was gradually lost in the higher degrees of epithelial dysplasia (Figure 2, B, D, F, H).


Altered β-catenin expression in oral mucosal dysplasia: a comparative study.

Silva BS, Castro CA, VON Zeidler SL, Sousa SC, Batista AC, Yamamoto-Silva FP - J Appl Oral Sci (2015)

β-catenin immunohistochemical expression in oral leukoplakia (OL) (mild, moderate and severe dysplasia) and normal oral mucosa. (A) β-catenin cytoplasmic membrane staining, in all three layers of mild dysplasia (x100), and (B) in the cytoplasm of basal and parabasal cells. (C, D) Illustrations of the β-catenin cytoplasmic membrane (CM) expression in the upper layers of moderate dysplasia, showing a decrease in cytoplasmic membrane expression, with more evident cytoplasmic localization in basal and parabasal cells (arrows head) (x100 - x400). (E, F) Weak membranous expression of β-catenin in parabasal and basal layers of severe dysplasia, with a significant increase in its expression, in the cytoplasm (arrows head) of cells of basaloid appearance (x100 - x400). (G, H) β-catenin in normal oral mucosa (x100 - x400)
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4621939&req=5

f02: β-catenin immunohistochemical expression in oral leukoplakia (OL) (mild, moderate and severe dysplasia) and normal oral mucosa. (A) β-catenin cytoplasmic membrane staining, in all three layers of mild dysplasia (x100), and (B) in the cytoplasm of basal and parabasal cells. (C, D) Illustrations of the β-catenin cytoplasmic membrane (CM) expression in the upper layers of moderate dysplasia, showing a decrease in cytoplasmic membrane expression, with more evident cytoplasmic localization in basal and parabasal cells (arrows head) (x100 - x400). (E, F) Weak membranous expression of β-catenin in parabasal and basal layers of severe dysplasia, with a significant increase in its expression, in the cytoplasm (arrows head) of cells of basaloid appearance (x100 - x400). (G, H) β-catenin in normal oral mucosa (x100 - x400)
Mentions: In general, β-catenin immunohistochemical staining was observed in the cytoplasmic membrane in all OL dysplastic groups (n=39; mild, moderate and severe dysplasia) (Figure 2). In mild, moderate and severe dysplasia, β-catenin staining was also detected in the cytoplasm in 51.2% (n=20) of OL cases (Figure 2, A-F). In addition, membranous expression of β-catenin in parabasal and basal layers was gradually lost in the higher degrees of epithelial dysplasia (Figure 2, B, D, F, H).

Bottom Line: The Chi-square test and the Fisher's exact test were used to verify possible differences in the β-catenin expression among the OL groups.A p-value of <0.05 was considered statistically significant.Results Membranous expression of β-catenin in parabasal and basal layers was gradually lost in the higher degrees of epithelial dysplasia.In normal oral mucosa, β-catenin was detected only in the cytoplasmic membrane.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Ciências Estomatológicas, Faculdade de Odontologia, Universidade Federal de Goiás, Goiânia, GO, Brazil.

ABSTRACT
Objective The current study aimed to investigate the β-catenin expression in oral leukoplakia (OL) with different degrees of epithelial dysplasia and normal oral mucosa.Material and Methods Formalin-fixed, paraffin-embedded tissue samples of 39 OL (mild dysplasia n=19, moderate dysplasia n=13, and severe dysplasia n=7), and 10 normal oral mucosa (control group) were submitted to immunohistochemical reactions to anti-β-catenin primary antibody. A qualitative β-catenin analysis was performed based on the percentage of positive cells. The cellular location and the epithelial layer were also considered. The Chi-square test and the Fisher's exact test were used to verify possible differences in the β-catenin expression among the OL groups. A p-value of <0.05 was considered statistically significant.Results Membranous expression of β-catenin in parabasal and basal layers was gradually lost in the higher degrees of epithelial dysplasia. In normal oral mucosa, β-catenin was detected only in the cytoplasmic membrane. However, a significant increase in cytoplasmic β-catenin could be observed between mild and moderate dysplasia (Fisher Exact test - p<0.001) and between mild and severe dysplasia (p<0.001).Conclusions The β-catenin cytoplasmic expression observed in this study may represent the initial stage of modifications in the E-cadherin-catenin complex, along with morphological cellular changes.

No MeSH data available.


Related in: MedlinePlus