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Biocompatibility and bioactivity of calcium silicate-based endodontic sealers in human dental pulp cells.

Mestieri LB, Gomes-Cornélio AL, Rodrigues EM, Salles LP, Bosso-Martelo R, Guerreiro-Tanomaru JM, Tanomaru-Filho M - J Appl Oral Sci (2015)

Bottom Line: The NR results demonstrated cell viability for all materials tested.MTAF and FC groups demonstrated a decrease in ALP activity when compared to CT at both periods of cell exposure.Conclusions The hDPCs were suitable for the evaluation of new endodontic materialsin vitro.MTAP may be considered a promising material for endodontic treatments.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Odontologia Restauradora, Escola de Odontologia, Universidade Estadual Paulista, Araraquara, SP, Brazil.

ABSTRACT
Mineral Trioxide Aggregate (MTA) is a calcium silicate-based material. New sealers have been developed based on calcium silicate as MTA Fillapex and MTA Plus.Objective The aim of this study was to evaluate biocompatibility and bioactivity of these two calcium silicate-based sealers in culture of human dental pulp cells (hDPCs).Material and Methods The cells were isolated from third molars extracted from a 16-year-old patient. Pulp tissue was sectioned into fragments with approximately 1 mm3 and kept in supplemented medium to obtain hDPCs adherent cultures. Cell characterization assays were performed to prove the osteogenic potential. The evaluated materials were: MTA Plus (MTAP); MTA Fillapex (MTAF) and FillCanal (FC). Biocompatibility was evaluated with MTT and Neutral Red (NR) assays, after hDPCs exposure for 24 h to different dilutions of each sealer extract (1:2, 1:3 and 1:4). Unexposed cells were the positive control (CT). Bioactivity was assessed by alkaline phosphatase (ALP) enzymatic assay in cells exposed for one and three days to sealer extracts (1:4 dilution). All data were analyzed by ANOVA and Tukey post-test (p≤0.05%).Results MTT and NR results showed suitable cell viability rates for MTAP at all dilutions (90-135%). Cells exposed to MTAF and FC (1:2 and 1:4 dilutions) showed significant low viability rate when compared to CT in MTT. The NR results demonstrated cell viability for all materials tested. In MTAP group, the cells ALP activity was similar to CT in one and three days of exposure to the material. MTAF and FC groups demonstrated a decrease in ALP activity when compared to CT at both periods of cell exposure.Conclusions The hDPCs were suitable for the evaluation of new endodontic materialsin vitro. MTAP may be considered a promising material for endodontic treatments.

No MeSH data available.


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Endodontic sealers evaluated
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f01: Endodontic sealers evaluated

Mentions: The experimental groups and the preparation proportions are described in Figure 1. Endodontic sealers were prepared according to manufactures instructions. FillCanal (FC) was prepared according to Grossman technique9, and MTA Plus (MTAP) was prepared with the vehicle for root canal filling consistency. Thereafter, 1 mL of each sealer was individually placed in wells of a 12-well plate (TPP, Techno Plastic Products, Zollstrasse, Trasadingen, Suíça) and spread to fill the bottom completely. The plates with the samples were kept in an incubator at 37°C, 95% humidity and 5% CO2 during 24 h for the initial setting time of the materials. After this period, the specimens were exposed to ultraviolet light (UV) in a laminar flow for 30 min each side to disinfection13. The wells were filled with 5 mL of α-MEM supplemented with 1% PenStrep and incubated at 37°C, 95% humidity and 5% CO2 for additional 24 h, in order to obtain the sealer extracts.


Biocompatibility and bioactivity of calcium silicate-based endodontic sealers in human dental pulp cells.

Mestieri LB, Gomes-Cornélio AL, Rodrigues EM, Salles LP, Bosso-Martelo R, Guerreiro-Tanomaru JM, Tanomaru-Filho M - J Appl Oral Sci (2015)

Endodontic sealers evaluated
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4621938&req=5

f01: Endodontic sealers evaluated
Mentions: The experimental groups and the preparation proportions are described in Figure 1. Endodontic sealers were prepared according to manufactures instructions. FillCanal (FC) was prepared according to Grossman technique9, and MTA Plus (MTAP) was prepared with the vehicle for root canal filling consistency. Thereafter, 1 mL of each sealer was individually placed in wells of a 12-well plate (TPP, Techno Plastic Products, Zollstrasse, Trasadingen, Suíça) and spread to fill the bottom completely. The plates with the samples were kept in an incubator at 37°C, 95% humidity and 5% CO2 during 24 h for the initial setting time of the materials. After this period, the specimens were exposed to ultraviolet light (UV) in a laminar flow for 30 min each side to disinfection13. The wells were filled with 5 mL of α-MEM supplemented with 1% PenStrep and incubated at 37°C, 95% humidity and 5% CO2 for additional 24 h, in order to obtain the sealer extracts.

Bottom Line: The NR results demonstrated cell viability for all materials tested.MTAF and FC groups demonstrated a decrease in ALP activity when compared to CT at both periods of cell exposure.Conclusions The hDPCs were suitable for the evaluation of new endodontic materialsin vitro.MTAP may be considered a promising material for endodontic treatments.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Odontologia Restauradora, Escola de Odontologia, Universidade Estadual Paulista, Araraquara, SP, Brazil.

ABSTRACT
Mineral Trioxide Aggregate (MTA) is a calcium silicate-based material. New sealers have been developed based on calcium silicate as MTA Fillapex and MTA Plus.Objective The aim of this study was to evaluate biocompatibility and bioactivity of these two calcium silicate-based sealers in culture of human dental pulp cells (hDPCs).Material and Methods The cells were isolated from third molars extracted from a 16-year-old patient. Pulp tissue was sectioned into fragments with approximately 1 mm3 and kept in supplemented medium to obtain hDPCs adherent cultures. Cell characterization assays were performed to prove the osteogenic potential. The evaluated materials were: MTA Plus (MTAP); MTA Fillapex (MTAF) and FillCanal (FC). Biocompatibility was evaluated with MTT and Neutral Red (NR) assays, after hDPCs exposure for 24 h to different dilutions of each sealer extract (1:2, 1:3 and 1:4). Unexposed cells were the positive control (CT). Bioactivity was assessed by alkaline phosphatase (ALP) enzymatic assay in cells exposed for one and three days to sealer extracts (1:4 dilution). All data were analyzed by ANOVA and Tukey post-test (p≤0.05%).Results MTT and NR results showed suitable cell viability rates for MTAP at all dilutions (90-135%). Cells exposed to MTAF and FC (1:2 and 1:4 dilutions) showed significant low viability rate when compared to CT in MTT. The NR results demonstrated cell viability for all materials tested. In MTAP group, the cells ALP activity was similar to CT in one and three days of exposure to the material. MTAF and FC groups demonstrated a decrease in ALP activity when compared to CT at both periods of cell exposure.Conclusions The hDPCs were suitable for the evaluation of new endodontic materialsin vitro. MTAP may be considered a promising material for endodontic treatments.

No MeSH data available.


Related in: MedlinePlus