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Acinar autolysis and mucous extravasation in human sublingual glands: a microscopic postmortem study.

Azevedo-Alanis LR, Tolentino Ede S, Assis GF, Cestari TM, Lara VS, Damante JH - J Appl Oral Sci (2015)

Bottom Line: Ultrastructural analysis was performed using transmission electron microscopy (TEM).No differences were observed between genders.Both findings are independent.

View Article: PubMed Central - PubMed

Affiliation: Escola de Saúde e Biociências, Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil.

ABSTRACT
Although some morphological investigations on aged human sublingual glands (HSG) found eventual phenomena identified as autolysis and mucous extravasation, the exact meaning of these findings has not been elucidated.Objective The aim of this work is to investigate whether acinar autolysis and mucous extravasation are related to the aging process in human sublingual glands. We also speculate if autolytic changes may assist forensic pathologists in determining time of death.Material and Methods 186 cadavers' glands were allocated to age groups: I (0-30 years); II (31-60), and III (61-90). Time and mode of death were also recorded. Acinar autolysis and mucous extravasation were classified as present or absent. Ultrastructural analysis was performed using transmission electron microscopy (TEM). Data were compared using Mann-Whitney U, Spearman's correlation coefficient, Kruskal-Wallis, and Dunn tests (p<0.05).Results There was correlation between age and acinar autolysis (r=0.38; p=0.0001). However, there was no correlation between autolysis and time of death. No differences were observed between genders. TEM showed mucous and serous cells presenting nuclear and membrane alterations and mucous cells were more susceptible to autolysis.Conclusion Acinar autolysis occurred in all age groups and increased with age while mucous extravasation was rarely found. Both findings are independent. Autolysis degrees in HSG could not be used to determine time of death.

No MeSH data available.


Related in: MedlinePlus

A: Acinar autolysis (*), Male, 49 years old (H.E. Original magnification: x100); B: Mucous extravasation (*), Female, 63 years old (H.E. Original magnification: x100). H.E.=Hematoxylin & eosin
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f02: A: Acinar autolysis (*), Male, 49 years old (H.E. Original magnification: x100); B: Mucous extravasation (*), Female, 63 years old (H.E. Original magnification: x100). H.E.=Hematoxylin & eosin

Mentions: An acinus was counted as autolyzed when it presented the minimum sign of loss of cell limit, regardless of the degree11 (Figure 2A). Mucous extravasation was sparsely distributed in the glandular parenchyma (Figure 2B).


Acinar autolysis and mucous extravasation in human sublingual glands: a microscopic postmortem study.

Azevedo-Alanis LR, Tolentino Ede S, Assis GF, Cestari TM, Lara VS, Damante JH - J Appl Oral Sci (2015)

A: Acinar autolysis (*), Male, 49 years old (H.E. Original magnification: x100); B: Mucous extravasation (*), Female, 63 years old (H.E. Original magnification: x100). H.E.=Hematoxylin & eosin
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4621937&req=5

f02: A: Acinar autolysis (*), Male, 49 years old (H.E. Original magnification: x100); B: Mucous extravasation (*), Female, 63 years old (H.E. Original magnification: x100). H.E.=Hematoxylin & eosin
Mentions: An acinus was counted as autolyzed when it presented the minimum sign of loss of cell limit, regardless of the degree11 (Figure 2A). Mucous extravasation was sparsely distributed in the glandular parenchyma (Figure 2B).

Bottom Line: Ultrastructural analysis was performed using transmission electron microscopy (TEM).No differences were observed between genders.Both findings are independent.

View Article: PubMed Central - PubMed

Affiliation: Escola de Saúde e Biociências, Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil.

ABSTRACT
Although some morphological investigations on aged human sublingual glands (HSG) found eventual phenomena identified as autolysis and mucous extravasation, the exact meaning of these findings has not been elucidated.Objective The aim of this work is to investigate whether acinar autolysis and mucous extravasation are related to the aging process in human sublingual glands. We also speculate if autolytic changes may assist forensic pathologists in determining time of death.Material and Methods 186 cadavers' glands were allocated to age groups: I (0-30 years); II (31-60), and III (61-90). Time and mode of death were also recorded. Acinar autolysis and mucous extravasation were classified as present or absent. Ultrastructural analysis was performed using transmission electron microscopy (TEM). Data were compared using Mann-Whitney U, Spearman's correlation coefficient, Kruskal-Wallis, and Dunn tests (p<0.05).Results There was correlation between age and acinar autolysis (r=0.38; p=0.0001). However, there was no correlation between autolysis and time of death. No differences were observed between genders. TEM showed mucous and serous cells presenting nuclear and membrane alterations and mucous cells were more susceptible to autolysis.Conclusion Acinar autolysis occurred in all age groups and increased with age while mucous extravasation was rarely found. Both findings are independent. Autolysis degrees in HSG could not be used to determine time of death.

No MeSH data available.


Related in: MedlinePlus