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Bacteria in a wood fungal disease: characterization of bacterial communities in wood tissues of esca-foliar symptomatic and asymptomatic grapevines.

Bruez E, Haidar R, Alou MT, Vallance J, Bertsch C, Mazet F, Fermaud M, Deschamps A, Guerin-Dubrana L, Compant S, Rey P - Front Microbiol (2015)

Bottom Line: Esca is a grapevine trunk disease (GTD) associated with different pathogenic fungi inhabiting the woody tissues.Although Bacillus sp. and Pantoea agglomerans were the two most commonly isolated species from all kinds of tissues, various other taxa were also isolated.This study showed, therefore, that bacterial communities differ according to the anatomical part (trunk or cordon) and/or the type of tissue (necrotic or non-necrotic) of wood of grapevine plants showing external symptoms of esca disease.

View Article: PubMed Central - PubMed

Affiliation: Institut des Sciences de la Vigne et du Vin, Université de Bordeaux Bordeaux, France ; UMR1065 Santé et Agroécologie du Vignoble, Bordeaux Sciences Agro Gradignan, France ; Institut National de la Recherche Agronomique, Institut des Sciences de la Vigne et du Vin, UMR1065 Santé et Agroécologie du Vignoble Villenave d'Ornon, France.

ABSTRACT
Esca is a grapevine trunk disease (GTD) associated with different pathogenic fungi inhabiting the woody tissues. Bacteria can also be found in such tissues and they may interact with these fungal colonizers. Although such types of microbial interactions have been observed for wood diseases in many trees, this has never been studied for grapevine. In this study, the bacterial microflora of different vine status (esca-symptomatic and asymptomatic), different anatomical part (trunk and cordon) and different type of tissues (necrotic or not) have been studied. Based on Single Strand Conformation Polymorphism (SSCP) analyses, data showed that (i) specific complexes of bacterial microflora colonize the wood of both necrotic and non-necrotic tissues of esca-foliar symptomatic and asymptomatic vines, and also that (ii) depending on the anatomical part of the plant, cordon or trunk, differences could be observed between the bacterial communities. Such differences were also revealed through the community-level physiological profiling (CLPP) with Biolog Ecoplates(TM). Two hundred seventeen bacterial strains were also isolated from plant samples and then assigned to bacterial species based on the 16S rRNA genes. Although Bacillus sp. and Pantoea agglomerans were the two most commonly isolated species from all kinds of tissues, various other taxa were also isolated. Inoculation of vine cuttings with 14 different bacterial species, and one GTD fungus, Neofusicoccum parvum, showed no impact of these bacteria on the size of the wood necroses caused by N. parvum. This study showed, therefore, that bacterial communities differ according to the anatomical part (trunk or cordon) and/or the type of tissue (necrotic or non-necrotic) of wood of grapevine plants showing external symptoms of esca disease. However, research into bacteria having a role in GTD development needs further studies.

No MeSH data available.


Related in: MedlinePlus

Principal component analysis (PCA) of the bacterial communities colonizing the non-necrotic and necrotic tissues of trunk and cordon of esca-foliar symptomatic and asymptomatic vines based on SSCP profiles. The variation (%) explained by each PCA axis is given in brackets. PCA eigenvalues indicate that the first two components, Dim 1 and Dim 2, account for 75% of the total bacterial variability. Squares correspond to individual SSCPs and ellipses to the 95% confidence intervals calculated for each community.
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Figure 2: Principal component analysis (PCA) of the bacterial communities colonizing the non-necrotic and necrotic tissues of trunk and cordon of esca-foliar symptomatic and asymptomatic vines based on SSCP profiles. The variation (%) explained by each PCA axis is given in brackets. PCA eigenvalues indicate that the first two components, Dim 1 and Dim 2, account for 75% of the total bacterial variability. Squares correspond to individual SSCPs and ellipses to the 95% confidence intervals calculated for each community.

Mentions: A PCA was carried out to compare the genetic structure of the bacterial communities in terms of tissue types, their localization and vine status. The distribution of samples on the principal plan generated by the PCA analysis is represented in Figure 2. Differences in the genetic structure of the bacterial communities were observed in the non-necrotic and necrotic tissues of the cordon for both symptomatic and asymptomatic plants. The different clusters are separated by the first PCA axis, Dim 1, which represents 53% of total bacterial variability, and the ellipses do not overlapped. Furthermore, bacterial communities colonizing the necrotic tissues of cordon from symptomatic and asymptomatic vines differ according to the Dim 1. No distinctive patterns were observed for the non-necrotic tissues of trunk.


Bacteria in a wood fungal disease: characterization of bacterial communities in wood tissues of esca-foliar symptomatic and asymptomatic grapevines.

Bruez E, Haidar R, Alou MT, Vallance J, Bertsch C, Mazet F, Fermaud M, Deschamps A, Guerin-Dubrana L, Compant S, Rey P - Front Microbiol (2015)

Principal component analysis (PCA) of the bacterial communities colonizing the non-necrotic and necrotic tissues of trunk and cordon of esca-foliar symptomatic and asymptomatic vines based on SSCP profiles. The variation (%) explained by each PCA axis is given in brackets. PCA eigenvalues indicate that the first two components, Dim 1 and Dim 2, account for 75% of the total bacterial variability. Squares correspond to individual SSCPs and ellipses to the 95% confidence intervals calculated for each community.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4621878&req=5

Figure 2: Principal component analysis (PCA) of the bacterial communities colonizing the non-necrotic and necrotic tissues of trunk and cordon of esca-foliar symptomatic and asymptomatic vines based on SSCP profiles. The variation (%) explained by each PCA axis is given in brackets. PCA eigenvalues indicate that the first two components, Dim 1 and Dim 2, account for 75% of the total bacterial variability. Squares correspond to individual SSCPs and ellipses to the 95% confidence intervals calculated for each community.
Mentions: A PCA was carried out to compare the genetic structure of the bacterial communities in terms of tissue types, their localization and vine status. The distribution of samples on the principal plan generated by the PCA analysis is represented in Figure 2. Differences in the genetic structure of the bacterial communities were observed in the non-necrotic and necrotic tissues of the cordon for both symptomatic and asymptomatic plants. The different clusters are separated by the first PCA axis, Dim 1, which represents 53% of total bacterial variability, and the ellipses do not overlapped. Furthermore, bacterial communities colonizing the necrotic tissues of cordon from symptomatic and asymptomatic vines differ according to the Dim 1. No distinctive patterns were observed for the non-necrotic tissues of trunk.

Bottom Line: Esca is a grapevine trunk disease (GTD) associated with different pathogenic fungi inhabiting the woody tissues.Although Bacillus sp. and Pantoea agglomerans were the two most commonly isolated species from all kinds of tissues, various other taxa were also isolated.This study showed, therefore, that bacterial communities differ according to the anatomical part (trunk or cordon) and/or the type of tissue (necrotic or non-necrotic) of wood of grapevine plants showing external symptoms of esca disease.

View Article: PubMed Central - PubMed

Affiliation: Institut des Sciences de la Vigne et du Vin, Université de Bordeaux Bordeaux, France ; UMR1065 Santé et Agroécologie du Vignoble, Bordeaux Sciences Agro Gradignan, France ; Institut National de la Recherche Agronomique, Institut des Sciences de la Vigne et du Vin, UMR1065 Santé et Agroécologie du Vignoble Villenave d'Ornon, France.

ABSTRACT
Esca is a grapevine trunk disease (GTD) associated with different pathogenic fungi inhabiting the woody tissues. Bacteria can also be found in such tissues and they may interact with these fungal colonizers. Although such types of microbial interactions have been observed for wood diseases in many trees, this has never been studied for grapevine. In this study, the bacterial microflora of different vine status (esca-symptomatic and asymptomatic), different anatomical part (trunk and cordon) and different type of tissues (necrotic or not) have been studied. Based on Single Strand Conformation Polymorphism (SSCP) analyses, data showed that (i) specific complexes of bacterial microflora colonize the wood of both necrotic and non-necrotic tissues of esca-foliar symptomatic and asymptomatic vines, and also that (ii) depending on the anatomical part of the plant, cordon or trunk, differences could be observed between the bacterial communities. Such differences were also revealed through the community-level physiological profiling (CLPP) with Biolog Ecoplates(TM). Two hundred seventeen bacterial strains were also isolated from plant samples and then assigned to bacterial species based on the 16S rRNA genes. Although Bacillus sp. and Pantoea agglomerans were the two most commonly isolated species from all kinds of tissues, various other taxa were also isolated. Inoculation of vine cuttings with 14 different bacterial species, and one GTD fungus, Neofusicoccum parvum, showed no impact of these bacteria on the size of the wood necroses caused by N. parvum. This study showed, therefore, that bacterial communities differ according to the anatomical part (trunk or cordon) and/or the type of tissue (necrotic or non-necrotic) of wood of grapevine plants showing external symptoms of esca disease. However, research into bacteria having a role in GTD development needs further studies.

No MeSH data available.


Related in: MedlinePlus