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Carcinogenic Liver Fluke Secretes Extracellular Vesicles That Promote Cholangiocytes to Adopt a Tumorigenic Phenotype.

Chaiyadet S, Sotillo J, Smout M, Cantacessi C, Jones MK, Johnson MS, Turnbull L, Whitchurch CB, Potriquet J, Laohaviroj M, Mulvenna J, Brindley PJ, Bethony JM, Laha T, Sripa B, Loukas A - J. Infect. Dis. (2015)

Bottom Line: Until now, the mechanism and effects of parasite protein entry into cholangiocytes was unknown.Using mass spectrometry we characterized the EV proteome and associated changes in cholangiocytes after EV uptake, and we detected EV proteins in bile of infected hamsters and humans.EVs internalized by cholangiocytes drove cell proliferation and IL-6 secretion and induced changes in protein expression associated with endocytosis, wound repair, and cancer.

View Article: PubMed Central - PubMed

Affiliation: Biomedical Sciences, Graduate School Centre for Biodiscovery and Molecular Development of Therapeutics, Australian Institute of Tropical Health and Medicine, James Cook University, Cairns.

No MeSH data available.


Related in: MedlinePlus

Extracellular vesicles (EVs) secreted by Opisthorchis viverrini. Transmission electron micrograph showing the presence of microvesicles after ultracentrifugation of O. viverrini excretory/secretory products. EV-like microvesicles of 40–100 nm can be observed.
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JIV291F1: Extracellular vesicles (EVs) secreted by Opisthorchis viverrini. Transmission electron micrograph showing the presence of microvesicles after ultracentrifugation of O. viverrini excretory/secretory products. EV-like microvesicles of 40–100 nm can be observed.

Mentions: After filtration and ultracentrifugation of ES products from adult O. viverrini, we identified microvesicular structures by using negative staining in transmission electron microscopy (Figure 1). These spherical vesicles had an external diameter of 40–180 nm, resembling EVs recently reported from other trematodes [13, 14].Figure 1.


Carcinogenic Liver Fluke Secretes Extracellular Vesicles That Promote Cholangiocytes to Adopt a Tumorigenic Phenotype.

Chaiyadet S, Sotillo J, Smout M, Cantacessi C, Jones MK, Johnson MS, Turnbull L, Whitchurch CB, Potriquet J, Laohaviroj M, Mulvenna J, Brindley PJ, Bethony JM, Laha T, Sripa B, Loukas A - J. Infect. Dis. (2015)

Extracellular vesicles (EVs) secreted by Opisthorchis viverrini. Transmission electron micrograph showing the presence of microvesicles after ultracentrifugation of O. viverrini excretory/secretory products. EV-like microvesicles of 40–100 nm can be observed.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4621255&req=5

JIV291F1: Extracellular vesicles (EVs) secreted by Opisthorchis viverrini. Transmission electron micrograph showing the presence of microvesicles after ultracentrifugation of O. viverrini excretory/secretory products. EV-like microvesicles of 40–100 nm can be observed.
Mentions: After filtration and ultracentrifugation of ES products from adult O. viverrini, we identified microvesicular structures by using negative staining in transmission electron microscopy (Figure 1). These spherical vesicles had an external diameter of 40–180 nm, resembling EVs recently reported from other trematodes [13, 14].Figure 1.

Bottom Line: Until now, the mechanism and effects of parasite protein entry into cholangiocytes was unknown.Using mass spectrometry we characterized the EV proteome and associated changes in cholangiocytes after EV uptake, and we detected EV proteins in bile of infected hamsters and humans.EVs internalized by cholangiocytes drove cell proliferation and IL-6 secretion and induced changes in protein expression associated with endocytosis, wound repair, and cancer.

View Article: PubMed Central - PubMed

Affiliation: Biomedical Sciences, Graduate School Centre for Biodiscovery and Molecular Development of Therapeutics, Australian Institute of Tropical Health and Medicine, James Cook University, Cairns.

No MeSH data available.


Related in: MedlinePlus