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Forkhead box protein A1 is a prognostic predictor and promotes tumor growth of gastric cancer.

Ren H, Zhang P, Tang Y, Wu M, Zhang W - Onco Targets Ther (2015)

Bottom Line: We found that the levels of FOXA1 protein and mRNA in gastric cancer tissues were significantly higher than those in matched tumor-adjacent tissues.In addition, FOXA1 knockdown remarkably inhibited cell proliferation and induced apoptosis in both SGC-7901 and MGC-803 cells.Mechanistically, we disclosed that the expression of Yes-associated protein was decreased accordingly after FOXA1 knockdown in both SGC-7901 and MGC-803 cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Union Hospital, Huazhong University of Science and Technology, Wuhan, People's Republic of China.

ABSTRACT
Previous studies have demonstrated the cancer-type specific role of forkhead box protein A1 (FOXA1) in human malignancies. However, the clinical significance of FOXA1 and its biological function in gastric cancer remain unknown. In this study, the expression of FOXA1 in 80 pairs of gastric cancer tissues and corresponding non-tumor tissues was analyzed using immunohistochemistry and quantitative real-time polymerase chain reaction. We found that the levels of FOXA1 protein and mRNA in gastric cancer tissues were significantly higher than those in matched tumor-adjacent tissues. Furthermore, clinical association analysis indicated that the positive expression of FOXA1 was associated with adverse clinicopathological characteristics of gastric cancer patients including poor tumor differentiation, large tumor size, and advanced tumor-node-metastasis tumor stage. Notably, gastric cancer patients with positive expression of FOXA1 had a poorer 5-year overall survival and recurrence-free survival. In addition, FOXA1 knockdown remarkably inhibited cell proliferation and induced apoptosis in both SGC-7901 and MGC-803 cells. In vivo studies indicated that FOXA1 knockdown prominently suppressed tumor growth of gastric cancer in a nude mouse xenograft model. Mechanistically, we disclosed that the expression of Yes-associated protein was decreased accordingly after FOXA1 knockdown in both SGC-7901 and MGC-803 cells. Taken together, our data suggest that FOXA1 may serve as a promising prognostic indicator and an attractive therapeutic target of gastric cancer.

No MeSH data available.


Related in: MedlinePlus

Expression levels of FOXA1 in gastric cancer tissues (T) and matched non-tumor tissues (NT).Notes: (A) Comparing differences in the expression levels of FOXA1 protein between gastric cancer tissues (T) and matched non-tumor tissues (NT). (B) qRT-PCR demonstrated that the mRNA level of FOXA1 in gastric cancer tissues was significantly increased as compared with that in matched non-tumor tissues. *P<0.05 by t-test.Abbreviations: FOXA1, forkhead box protein A1; NT, non-tumor tissues; qRT-PCR, quantitative reverse transcription polymerase chain reaction.
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f2-ott-8-3029: Expression levels of FOXA1 in gastric cancer tissues (T) and matched non-tumor tissues (NT).Notes: (A) Comparing differences in the expression levels of FOXA1 protein between gastric cancer tissues (T) and matched non-tumor tissues (NT). (B) qRT-PCR demonstrated that the mRNA level of FOXA1 in gastric cancer tissues was significantly increased as compared with that in matched non-tumor tissues. *P<0.05 by t-test.Abbreviations: FOXA1, forkhead box protein A1; NT, non-tumor tissues; qRT-PCR, quantitative reverse transcription polymerase chain reaction.

Mentions: Immunohistochemical staining was performed to investigate the expression of FOXA1 between gastric cancer tissues and matched tumor-adjacent tissues. As shown in Figure 1, negative staining of FOXA1 was observed in adjacent noncancerous tissue (Figure 1A), while positive staining of FOXA1 with nuclear location was presented in gastric cancer tissues (Figure 1B–D). The comparison of immunohistochemistry scores indicated that the level of FOXA1 protein in gastric cancer tissues was significantly upregulated as compared with adjacent noncancerous tissues (P<0.05, Figure 2A). Furthermore, 20 randomly selected cases were subjected to quantitative reverse transcription polymerase chain reaction for FOXA1 mRNA. We found the expression of FOXA1 mRNA was significantly higher in gastric cancer tissues than that in corresponding tumor-adjacent tissues (P<0.05, Figure 2B). There results indicate an oncogenic role of FOXA1 in gastric cancer.


Forkhead box protein A1 is a prognostic predictor and promotes tumor growth of gastric cancer.

Ren H, Zhang P, Tang Y, Wu M, Zhang W - Onco Targets Ther (2015)

Expression levels of FOXA1 in gastric cancer tissues (T) and matched non-tumor tissues (NT).Notes: (A) Comparing differences in the expression levels of FOXA1 protein between gastric cancer tissues (T) and matched non-tumor tissues (NT). (B) qRT-PCR demonstrated that the mRNA level of FOXA1 in gastric cancer tissues was significantly increased as compared with that in matched non-tumor tissues. *P<0.05 by t-test.Abbreviations: FOXA1, forkhead box protein A1; NT, non-tumor tissues; qRT-PCR, quantitative reverse transcription polymerase chain reaction.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4621220&req=5

f2-ott-8-3029: Expression levels of FOXA1 in gastric cancer tissues (T) and matched non-tumor tissues (NT).Notes: (A) Comparing differences in the expression levels of FOXA1 protein between gastric cancer tissues (T) and matched non-tumor tissues (NT). (B) qRT-PCR demonstrated that the mRNA level of FOXA1 in gastric cancer tissues was significantly increased as compared with that in matched non-tumor tissues. *P<0.05 by t-test.Abbreviations: FOXA1, forkhead box protein A1; NT, non-tumor tissues; qRT-PCR, quantitative reverse transcription polymerase chain reaction.
Mentions: Immunohistochemical staining was performed to investigate the expression of FOXA1 between gastric cancer tissues and matched tumor-adjacent tissues. As shown in Figure 1, negative staining of FOXA1 was observed in adjacent noncancerous tissue (Figure 1A), while positive staining of FOXA1 with nuclear location was presented in gastric cancer tissues (Figure 1B–D). The comparison of immunohistochemistry scores indicated that the level of FOXA1 protein in gastric cancer tissues was significantly upregulated as compared with adjacent noncancerous tissues (P<0.05, Figure 2A). Furthermore, 20 randomly selected cases were subjected to quantitative reverse transcription polymerase chain reaction for FOXA1 mRNA. We found the expression of FOXA1 mRNA was significantly higher in gastric cancer tissues than that in corresponding tumor-adjacent tissues (P<0.05, Figure 2B). There results indicate an oncogenic role of FOXA1 in gastric cancer.

Bottom Line: We found that the levels of FOXA1 protein and mRNA in gastric cancer tissues were significantly higher than those in matched tumor-adjacent tissues.In addition, FOXA1 knockdown remarkably inhibited cell proliferation and induced apoptosis in both SGC-7901 and MGC-803 cells.Mechanistically, we disclosed that the expression of Yes-associated protein was decreased accordingly after FOXA1 knockdown in both SGC-7901 and MGC-803 cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Gastroenterology, Union Hospital, Huazhong University of Science and Technology, Wuhan, People's Republic of China.

ABSTRACT
Previous studies have demonstrated the cancer-type specific role of forkhead box protein A1 (FOXA1) in human malignancies. However, the clinical significance of FOXA1 and its biological function in gastric cancer remain unknown. In this study, the expression of FOXA1 in 80 pairs of gastric cancer tissues and corresponding non-tumor tissues was analyzed using immunohistochemistry and quantitative real-time polymerase chain reaction. We found that the levels of FOXA1 protein and mRNA in gastric cancer tissues were significantly higher than those in matched tumor-adjacent tissues. Furthermore, clinical association analysis indicated that the positive expression of FOXA1 was associated with adverse clinicopathological characteristics of gastric cancer patients including poor tumor differentiation, large tumor size, and advanced tumor-node-metastasis tumor stage. Notably, gastric cancer patients with positive expression of FOXA1 had a poorer 5-year overall survival and recurrence-free survival. In addition, FOXA1 knockdown remarkably inhibited cell proliferation and induced apoptosis in both SGC-7901 and MGC-803 cells. In vivo studies indicated that FOXA1 knockdown prominently suppressed tumor growth of gastric cancer in a nude mouse xenograft model. Mechanistically, we disclosed that the expression of Yes-associated protein was decreased accordingly after FOXA1 knockdown in both SGC-7901 and MGC-803 cells. Taken together, our data suggest that FOXA1 may serve as a promising prognostic indicator and an attractive therapeutic target of gastric cancer.

No MeSH data available.


Related in: MedlinePlus