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Listeria monocytogenes-associated biliary tract infections: a study of 12 consecutive cases and review.

Charlier C, Fevre C, Travier L, Cazenave B, Bracq-Dieye H, Podevin J, Assomany D, Guilbert L, Bossard C, Carpentier F, Cales V, Leclercq A, Lecuit M - Medicine (Baltimore) (2014)

Bottom Line: Gallbladder histological lesions were analyzed in 3 patients and evidenced acute, chronic, or necrotic exacerbation of chronic infection.Genoserogroup of the 12 available strains were IVb (n=6), IIb (n=5), and IIa (n=1).Their survival in the bile was not enhanced when compared with isolates from other listeriosis cases.

View Article: PubMed Central - PubMed

Affiliation: Institut Pasteur, Biology of Infection Unit (CC, CF, LT, ML); Institut Pasteur French National Reference Center and WHO Collaborating Center for Listeria (CC, BC, HBD, AL, ML); Inserm U1117 (CC, CF, LT, ML); Université Paris Descartes, Sorbonne Paris Cité, Centre d'Infectiologie Necker-Pasteur, Hôpital Necker-Enfants Malades, Institut Imagine (CC, ML); Service de Chirurgie viscérale, Centre Hospitalier Universitaire de Nantes (JP); Service de Chirurgie viscérale et digestive, Centre Hospitalier de Roubaix (DA, LG); Service d'Anatomopathologie et Cytologie, Centre Hospitalier Universitaire de Nantes (CB); Service d'Anatomopathologie et Cytologie, Centre Hospitalier de Roubaix (FC); and Service d'Anatomopathologie et Cytologie, Centre Hospitalier de Pau (VC).

ABSTRACT
At present, little is known regarding Listeria monocytogenes-associated biliary tract infection, a rare form of listeriosis.In this article, we will study 12 culture-proven cases reported to the French National Reference Center for Listeria from 1996 to 2013 and review the 8 previously published cases.Twenty cases were studied: 17 cholecystitis, 2 cholangitis, and 1 biliary cyst infection. Half were men with a median age of 69 years (32-85). Comorbidities were present in 80%, including cirrhosis, rheumatoid arthritis, and diabetes. Five patients received immunosuppressive therapy, including corticosteroids and anti-tumor necrosis factor biotherapies. Half were afebrile. Blood cultures were positive in 60% (3/5). Gallbladder histological lesions were analyzed in 3 patients and evidenced acute, chronic, or necrotic exacerbation of chronic infection. Genoserogroup of the 12 available strains were IVb (n=6), IIb (n=5), and IIa (n=1). Their survival in the bile was not enhanced when compared with isolates from other listeriosis cases. Adverse outcome was reported in 33% (5/15): 3 deaths, 1 recurrence; 75% of the patients with adverse outcome received inadequate antimicrobial therapy (P=0.033).Biliary tract listeriosis is a severe infection associated with high mortality in patients not treated with appropriate therapy. This study provides medical relevance to in vitro and animal studies that had shown Listeria monocytogenes ability to survive in bile and induce overt biliary infections.

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Related in: MedlinePlus

Biofilm formation in 12 French isolates and in 30 strains selected at random among isolates received in 2012 from the National Reference Center for Listeria from patients with septicemia (S), central nervous system (CNS), and maternal–neonatal (MN) infections. P values were determined as compared to S, CNS, and MN isolates (Mann–Whitney test). BHI =  brain heart infusion, BTI = bile tract infections.
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Figure 3: Biofilm formation in 12 French isolates and in 30 strains selected at random among isolates received in 2012 from the National Reference Center for Listeria from patients with septicemia (S), central nervous system (CNS), and maternal–neonatal (MN) infections. P values were determined as compared to S, CNS, and MN isolates (Mann–Whitney test). BHI =  brain heart infusion, BTI = bile tract infections.

Mentions: No difference in biofilm ability among strains was observed with or without pork bile at pH 7 reflecting the gallbladder conditions. In the presence of pork bile, at pH 5 reflecting duodenal conditions, bile tract isolates had significantly lower biofilm ability than those from S, CNS, and MN infections (P = 0.001) (Figure 3).


Listeria monocytogenes-associated biliary tract infections: a study of 12 consecutive cases and review.

Charlier C, Fevre C, Travier L, Cazenave B, Bracq-Dieye H, Podevin J, Assomany D, Guilbert L, Bossard C, Carpentier F, Cales V, Leclercq A, Lecuit M - Medicine (Baltimore) (2014)

Biofilm formation in 12 French isolates and in 30 strains selected at random among isolates received in 2012 from the National Reference Center for Listeria from patients with septicemia (S), central nervous system (CNS), and maternal–neonatal (MN) infections. P values were determined as compared to S, CNS, and MN isolates (Mann–Whitney test). BHI =  brain heart infusion, BTI = bile tract infections.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4616294&req=5

Figure 3: Biofilm formation in 12 French isolates and in 30 strains selected at random among isolates received in 2012 from the National Reference Center for Listeria from patients with septicemia (S), central nervous system (CNS), and maternal–neonatal (MN) infections. P values were determined as compared to S, CNS, and MN isolates (Mann–Whitney test). BHI =  brain heart infusion, BTI = bile tract infections.
Mentions: No difference in biofilm ability among strains was observed with or without pork bile at pH 7 reflecting the gallbladder conditions. In the presence of pork bile, at pH 5 reflecting duodenal conditions, bile tract isolates had significantly lower biofilm ability than those from S, CNS, and MN infections (P = 0.001) (Figure 3).

Bottom Line: Gallbladder histological lesions were analyzed in 3 patients and evidenced acute, chronic, or necrotic exacerbation of chronic infection.Genoserogroup of the 12 available strains were IVb (n=6), IIb (n=5), and IIa (n=1).Their survival in the bile was not enhanced when compared with isolates from other listeriosis cases.

View Article: PubMed Central - PubMed

Affiliation: Institut Pasteur, Biology of Infection Unit (CC, CF, LT, ML); Institut Pasteur French National Reference Center and WHO Collaborating Center for Listeria (CC, BC, HBD, AL, ML); Inserm U1117 (CC, CF, LT, ML); Université Paris Descartes, Sorbonne Paris Cité, Centre d'Infectiologie Necker-Pasteur, Hôpital Necker-Enfants Malades, Institut Imagine (CC, ML); Service de Chirurgie viscérale, Centre Hospitalier Universitaire de Nantes (JP); Service de Chirurgie viscérale et digestive, Centre Hospitalier de Roubaix (DA, LG); Service d'Anatomopathologie et Cytologie, Centre Hospitalier Universitaire de Nantes (CB); Service d'Anatomopathologie et Cytologie, Centre Hospitalier de Roubaix (FC); and Service d'Anatomopathologie et Cytologie, Centre Hospitalier de Pau (VC).

ABSTRACT
At present, little is known regarding Listeria monocytogenes-associated biliary tract infection, a rare form of listeriosis.In this article, we will study 12 culture-proven cases reported to the French National Reference Center for Listeria from 1996 to 2013 and review the 8 previously published cases.Twenty cases were studied: 17 cholecystitis, 2 cholangitis, and 1 biliary cyst infection. Half were men with a median age of 69 years (32-85). Comorbidities were present in 80%, including cirrhosis, rheumatoid arthritis, and diabetes. Five patients received immunosuppressive therapy, including corticosteroids and anti-tumor necrosis factor biotherapies. Half were afebrile. Blood cultures were positive in 60% (3/5). Gallbladder histological lesions were analyzed in 3 patients and evidenced acute, chronic, or necrotic exacerbation of chronic infection. Genoserogroup of the 12 available strains were IVb (n=6), IIb (n=5), and IIa (n=1). Their survival in the bile was not enhanced when compared with isolates from other listeriosis cases. Adverse outcome was reported in 33% (5/15): 3 deaths, 1 recurrence; 75% of the patients with adverse outcome received inadequate antimicrobial therapy (P=0.033).Biliary tract listeriosis is a severe infection associated with high mortality in patients not treated with appropriate therapy. This study provides medical relevance to in vitro and animal studies that had shown Listeria monocytogenes ability to survive in bile and induce overt biliary infections.

Show MeSH
Related in: MedlinePlus