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Phytomedical investigation of Najas minor All. in the view of the chemical constituents.

Topuzovic MD, Radojevic ID, Dekic MS, Radulovic NS, Vasic SM, Comic LR, Licina BZ - EXCLI J (2015)

Bottom Line: Ethyl acetate extract had a significantly greater amount of total phenolics, flavonoids and tannins.The ethyl acetate extract effected only G+ bacteria, but the biofilm formation of G-bacteria was suppressed.There was a connection between those in vivo and in vitro effects against pathogenic bacterial biofilm formation.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Kragujevac, Serbia.

ABSTRACT
Plants are an abundant natural source of effective antibiotic compounds. Phytomedical investigations of certain plants haven't still been conducted. One of them is Najas minor (N. minor), an aquatic plant with confirmed allelopathy. Research conducted in this study showed the influence of water and ethyl acetate extracts of N. minor on microorganisms, in the view of chemical profiling of volatile constituents and the concentrations of total phenols, flavonoids and tannins. Antimicrobial activity was defined by determining minimum inhibitory and minimum microbicidal concentrations using microdilution method. Influence on bacterial biofilm formation was performed by tissue culture plate method. The total phenolics, flavonoids and condensed tannins were determined by Folin-Ciocalteu, aluminum chloride and butanol-HCl colorimetric methods. Chemical profiling of volatile constituents was investigated by GC and GC-MS. Water extract didn't have antimicrobial activity below 5000 µg/mL. Ethyl acetate extract has shown strong antimicrobial activity on G+ bacteria - Staphylococcus aureus PMFKGB12 and Bacillus subtilis (MIC < 78.13 µg/mL). The best antibiofilm activity was obtained on Escherichia coli ATCC25922 (BIC50 at 719 µg/mL). Water extract had higher yield. Ethyl acetate extract had a significantly greater amount of total phenolics, flavonoids and tannins. As major constituent hexahydrofarnesyl acetone was identified. The ethyl acetate extract effected only G+ bacteria, but the biofilm formation of G-bacteria was suppressed. There was a connection between those in vivo and in vitro effects against pathogenic bacterial biofilm formation. All of this points to a so far unexplored potential of N. minor.

No MeSH data available.


Related in: MedlinePlus

Antimicrobial activity of water and ethyl acetate extracts of N. minor against tested microorganisms based on microdilution method
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T1: Antimicrobial activity of water and ethyl acetate extracts of N. minor against tested microorganisms based on microdilution method

Mentions: Table 1(Tab. 1) shows the list of tested microorganisms. Complete clinical isolates were donated by the Institute of Public Health in Kragujevac. Other microorganisms used were from the collection of Microbiology Laboratory of Faculty of Science, University of Kragujevac.


Phytomedical investigation of Najas minor All. in the view of the chemical constituents.

Topuzovic MD, Radojevic ID, Dekic MS, Radulovic NS, Vasic SM, Comic LR, Licina BZ - EXCLI J (2015)

Antimicrobial activity of water and ethyl acetate extracts of N. minor against tested microorganisms based on microdilution method
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4616246&req=5

T1: Antimicrobial activity of water and ethyl acetate extracts of N. minor against tested microorganisms based on microdilution method
Mentions: Table 1(Tab. 1) shows the list of tested microorganisms. Complete clinical isolates were donated by the Institute of Public Health in Kragujevac. Other microorganisms used were from the collection of Microbiology Laboratory of Faculty of Science, University of Kragujevac.

Bottom Line: Ethyl acetate extract had a significantly greater amount of total phenolics, flavonoids and tannins.The ethyl acetate extract effected only G+ bacteria, but the biofilm formation of G-bacteria was suppressed.There was a connection between those in vivo and in vitro effects against pathogenic bacterial biofilm formation.

View Article: PubMed Central - PubMed

Affiliation: Department of Biology and Ecology, Faculty of Science, University of Kragujevac, Kragujevac, Serbia.

ABSTRACT
Plants are an abundant natural source of effective antibiotic compounds. Phytomedical investigations of certain plants haven't still been conducted. One of them is Najas minor (N. minor), an aquatic plant with confirmed allelopathy. Research conducted in this study showed the influence of water and ethyl acetate extracts of N. minor on microorganisms, in the view of chemical profiling of volatile constituents and the concentrations of total phenols, flavonoids and tannins. Antimicrobial activity was defined by determining minimum inhibitory and minimum microbicidal concentrations using microdilution method. Influence on bacterial biofilm formation was performed by tissue culture plate method. The total phenolics, flavonoids and condensed tannins were determined by Folin-Ciocalteu, aluminum chloride and butanol-HCl colorimetric methods. Chemical profiling of volatile constituents was investigated by GC and GC-MS. Water extract didn't have antimicrobial activity below 5000 µg/mL. Ethyl acetate extract has shown strong antimicrobial activity on G+ bacteria - Staphylococcus aureus PMFKGB12 and Bacillus subtilis (MIC < 78.13 µg/mL). The best antibiofilm activity was obtained on Escherichia coli ATCC25922 (BIC50 at 719 µg/mL). Water extract had higher yield. Ethyl acetate extract had a significantly greater amount of total phenolics, flavonoids and tannins. As major constituent hexahydrofarnesyl acetone was identified. The ethyl acetate extract effected only G+ bacteria, but the biofilm formation of G-bacteria was suppressed. There was a connection between those in vivo and in vitro effects against pathogenic bacterial biofilm formation. All of this points to a so far unexplored potential of N. minor.

No MeSH data available.


Related in: MedlinePlus