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Phytochemical Characterization of Veronica officinalis L., V. teucrium L. and V. orchidea Crantz from Romania and Their Antioxidant and Antimicrobial Properties.

Mocan A, Vodnar DC, Vlase L, Crișan O, Gheldiu AM, Crișan G - Int J Mol Sci (2015)

Bottom Line: More than that, hispidulin, eupatorin and eupatilin were detected for the first time in the Veronica genus.Regarding the antimicrobial activity of the investigated species, Staphylococcus aureus, Listeria monocytogenes and Listeria ivanovii were the most sensitive strains with MIC values between 3.9 and 15.62 mg/mL.The results obtained by this study may serve to promote better use of representatives from the genus Veronica as antioxidant and antimicrobial agents.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Botany, Iuliu Hațieganu University of Medicine and Pharmacy, 23 Ghe. Marinescu Street, Cluj-Napoca 400010, Romania. mocan.andrei@umfcluj.ro.

ABSTRACT
Aerial parts of Veronica species are used in Romanian traditional medicine for the treatment of various conditions like kidney diseases, cough, and catarrh, and are known for their wound-healing properties. In the present study, the phenolic and sterolic content and the antioxidant and antimicrobial activities of three Veronica species (Plantaginaceae), V. officinalis L., V. teucrium L. and V. orchidea Crantz, were studied. The identification and quantification of several phenolic compounds and phytosterols were performed using LC/MS techniques and the main components were p-coumaric acid, ferulic acid, luteoline, hispidulin and β-sitosterol. More than that, hispidulin, eupatorin and eupatilin were detected for the first time in the Veronica genus. Nevertheless, representatives of the Veronica genus were never investigated in terms of their phytosterol content. The antioxidant potential investigated by Trolox equivelents antioxidant capacity (TEAC) and EPR spectroscopy revealed that V. officinalis and V. orchidea extracts presented similar antioxidant capacities, whilst the values registered for V. teucrium extract are lower. Regarding the antimicrobial activity of the investigated species, Staphylococcus aureus, Listeria monocytogenes and Listeria ivanovii were the most sensitive strains with MIC values between 3.9 and 15.62 mg/mL. The results obtained by this study may serve to promote better use of representatives from the genus Veronica as antioxidant and antimicrobial agents.

No MeSH data available.


Related in: MedlinePlus

Tipical chromatogram of the six analyzed flavones: (1) jaceosidin; (2) hispidulin; (3) eupalitin; (4) eupatorin; (5) casticin and (6) acacetin.
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ijms-16-21109-f002: Tipical chromatogram of the six analyzed flavones: (1) jaceosidin; (2) hispidulin; (3) eupalitin; (4) eupatorin; (5) casticin and (6) acacetin.

Mentions: Under the described chromatographic conditions, the compounds eluted in less than 10 min: jaceosidin (RT = 2.9 min), hispidulin (RT = 4.2 min), eupalitin (RT = 7.05 min), eupatorin (RT = 7.6 min), casticin (RT = 8.05 min) and acacetin (RT = 9.8 min), as seen in Figure 2. The ions monitorized in the MS method are presented in Table 2. Because in the ionization conditions, all flavones have lost a proton, the parent ions detected are always in the form [M − H]−. In the process of mass-spectrometry, the pseudo-molecular ions of the flavones (329.3 for jaceosidin, 299.2 for hispidulin, 343.3 for eupatilin, 343.3 for eupatorin, 373.3 for casticin and 283.3 for acacetin) have been fragmented, and based on their daughter ions from the MS spectrum (Table 2) the extracted chromatograms of each compound were constructed for quantification, taking into account the intensity of major ions in the mass spectrum.


Phytochemical Characterization of Veronica officinalis L., V. teucrium L. and V. orchidea Crantz from Romania and Their Antioxidant and Antimicrobial Properties.

Mocan A, Vodnar DC, Vlase L, Crișan O, Gheldiu AM, Crișan G - Int J Mol Sci (2015)

Tipical chromatogram of the six analyzed flavones: (1) jaceosidin; (2) hispidulin; (3) eupalitin; (4) eupatorin; (5) casticin and (6) acacetin.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4613244&req=5

ijms-16-21109-f002: Tipical chromatogram of the six analyzed flavones: (1) jaceosidin; (2) hispidulin; (3) eupalitin; (4) eupatorin; (5) casticin and (6) acacetin.
Mentions: Under the described chromatographic conditions, the compounds eluted in less than 10 min: jaceosidin (RT = 2.9 min), hispidulin (RT = 4.2 min), eupalitin (RT = 7.05 min), eupatorin (RT = 7.6 min), casticin (RT = 8.05 min) and acacetin (RT = 9.8 min), as seen in Figure 2. The ions monitorized in the MS method are presented in Table 2. Because in the ionization conditions, all flavones have lost a proton, the parent ions detected are always in the form [M − H]−. In the process of mass-spectrometry, the pseudo-molecular ions of the flavones (329.3 for jaceosidin, 299.2 for hispidulin, 343.3 for eupatilin, 343.3 for eupatorin, 373.3 for casticin and 283.3 for acacetin) have been fragmented, and based on their daughter ions from the MS spectrum (Table 2) the extracted chromatograms of each compound were constructed for quantification, taking into account the intensity of major ions in the mass spectrum.

Bottom Line: More than that, hispidulin, eupatorin and eupatilin were detected for the first time in the Veronica genus.Regarding the antimicrobial activity of the investigated species, Staphylococcus aureus, Listeria monocytogenes and Listeria ivanovii were the most sensitive strains with MIC values between 3.9 and 15.62 mg/mL.The results obtained by this study may serve to promote better use of representatives from the genus Veronica as antioxidant and antimicrobial agents.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmaceutical Botany, Iuliu Hațieganu University of Medicine and Pharmacy, 23 Ghe. Marinescu Street, Cluj-Napoca 400010, Romania. mocan.andrei@umfcluj.ro.

ABSTRACT
Aerial parts of Veronica species are used in Romanian traditional medicine for the treatment of various conditions like kidney diseases, cough, and catarrh, and are known for their wound-healing properties. In the present study, the phenolic and sterolic content and the antioxidant and antimicrobial activities of three Veronica species (Plantaginaceae), V. officinalis L., V. teucrium L. and V. orchidea Crantz, were studied. The identification and quantification of several phenolic compounds and phytosterols were performed using LC/MS techniques and the main components were p-coumaric acid, ferulic acid, luteoline, hispidulin and β-sitosterol. More than that, hispidulin, eupatorin and eupatilin were detected for the first time in the Veronica genus. Nevertheless, representatives of the Veronica genus were never investigated in terms of their phytosterol content. The antioxidant potential investigated by Trolox equivelents antioxidant capacity (TEAC) and EPR spectroscopy revealed that V. officinalis and V. orchidea extracts presented similar antioxidant capacities, whilst the values registered for V. teucrium extract are lower. Regarding the antimicrobial activity of the investigated species, Staphylococcus aureus, Listeria monocytogenes and Listeria ivanovii were the most sensitive strains with MIC values between 3.9 and 15.62 mg/mL. The results obtained by this study may serve to promote better use of representatives from the genus Veronica as antioxidant and antimicrobial agents.

No MeSH data available.


Related in: MedlinePlus