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A Simple and Effective Mass Spectrometric Approach to Identify the Adulteration of the Mediterranean Diet Component Extra-Virgin Olive Oil with Corn Oil.

Di Girolamo F, Masotti A, Lante I, Scapaticci M, Calvano CD, Zambonin C, Muraca M, Putignani L - Int J Mol Sci (2015)

Bottom Line: Extra virgin olive oil (EVOO) with its nutraceutical characteristics substantially contributes as a major nutrient to the health benefit of the Mediterranean diet.Unfortunately, the adulteration of EVOO with less expensive oils (e.g., peanut and corn oils), has become one of the biggest source of agricultural fraud in the European Union, with important health implications for consumers, mainly due to the introduction of seed oil-derived allergens causing, especially in children, severe food allergy phenomena.In this regard, revealing adulterations of EVOO is of fundamental importance for health care and prevention reasons, especially in children.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Medicine, Bambino Gesù Children's Hospital, Scientific Institute for Research, Hospitalization and Health Care (IRCCS), Piazza Sant'Onofrio 4, Rome 00165, Italy. francesco.digirolamo@opbg.net.

ABSTRACT
Extra virgin olive oil (EVOO) with its nutraceutical characteristics substantially contributes as a major nutrient to the health benefit of the Mediterranean diet. Unfortunately, the adulteration of EVOO with less expensive oils (e.g., peanut and corn oils), has become one of the biggest source of agricultural fraud in the European Union, with important health implications for consumers, mainly due to the introduction of seed oil-derived allergens causing, especially in children, severe food allergy phenomena. In this regard, revealing adulterations of EVOO is of fundamental importance for health care and prevention reasons, especially in children. To this aim, effective analytical methods to assess EVOO purity are necessary. Here, we propose a simple, rapid, robust and very sensitive method for non-specialized mass spectrometric laboratory, based on the matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) coupled to unsupervised hierarchical clustering (UHC), principal component (PCA) and Pearson's correlation analyses, to reveal corn oil (CO) adulterations in EVOO at very low levels (down to 0.5%).

No MeSH data available.


Related in: MedlinePlus

3D scatter plots of the first three PC for the five mixtures (20%, 10%, 5%, 1% and 0.5%), obtained by adding CO (A–E) to EVOO. All EVOO, CO and oil adulterations replicas grouped inside correct cluster.
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ijms-16-20896-f004: 3D scatter plots of the first three PC for the five mixtures (20%, 10%, 5%, 1% and 0.5%), obtained by adding CO (A–E) to EVOO. All EVOO, CO and oil adulterations replicas grouped inside correct cluster.

Mentions: Subsequently, we grouped all spectra, from EVOO adulteration/contamination, within consistent PCA clusters for EVOO/CO (Figure 4, Panels A–E) mixtures.


A Simple and Effective Mass Spectrometric Approach to Identify the Adulteration of the Mediterranean Diet Component Extra-Virgin Olive Oil with Corn Oil.

Di Girolamo F, Masotti A, Lante I, Scapaticci M, Calvano CD, Zambonin C, Muraca M, Putignani L - Int J Mol Sci (2015)

3D scatter plots of the first three PC for the five mixtures (20%, 10%, 5%, 1% and 0.5%), obtained by adding CO (A–E) to EVOO. All EVOO, CO and oil adulterations replicas grouped inside correct cluster.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4613234&req=5

ijms-16-20896-f004: 3D scatter plots of the first three PC for the five mixtures (20%, 10%, 5%, 1% and 0.5%), obtained by adding CO (A–E) to EVOO. All EVOO, CO and oil adulterations replicas grouped inside correct cluster.
Mentions: Subsequently, we grouped all spectra, from EVOO adulteration/contamination, within consistent PCA clusters for EVOO/CO (Figure 4, Panels A–E) mixtures.

Bottom Line: Extra virgin olive oil (EVOO) with its nutraceutical characteristics substantially contributes as a major nutrient to the health benefit of the Mediterranean diet.Unfortunately, the adulteration of EVOO with less expensive oils (e.g., peanut and corn oils), has become one of the biggest source of agricultural fraud in the European Union, with important health implications for consumers, mainly due to the introduction of seed oil-derived allergens causing, especially in children, severe food allergy phenomena.In this regard, revealing adulterations of EVOO is of fundamental importance for health care and prevention reasons, especially in children.

View Article: PubMed Central - PubMed

Affiliation: Department of Laboratory Medicine, Bambino Gesù Children's Hospital, Scientific Institute for Research, Hospitalization and Health Care (IRCCS), Piazza Sant'Onofrio 4, Rome 00165, Italy. francesco.digirolamo@opbg.net.

ABSTRACT
Extra virgin olive oil (EVOO) with its nutraceutical characteristics substantially contributes as a major nutrient to the health benefit of the Mediterranean diet. Unfortunately, the adulteration of EVOO with less expensive oils (e.g., peanut and corn oils), has become one of the biggest source of agricultural fraud in the European Union, with important health implications for consumers, mainly due to the introduction of seed oil-derived allergens causing, especially in children, severe food allergy phenomena. In this regard, revealing adulterations of EVOO is of fundamental importance for health care and prevention reasons, especially in children. To this aim, effective analytical methods to assess EVOO purity are necessary. Here, we propose a simple, rapid, robust and very sensitive method for non-specialized mass spectrometric laboratory, based on the matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) coupled to unsupervised hierarchical clustering (UHC), principal component (PCA) and Pearson's correlation analyses, to reveal corn oil (CO) adulterations in EVOO at very low levels (down to 0.5%).

No MeSH data available.


Related in: MedlinePlus