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Assembly and positioning of actomyosin rings by contractility and planar cell polarity.

Sehring IM, Recho P, Denker E, Kourakis M, Mathiesen B, Hannezo E, Dong B, Jiang D - Elife (2015)

Bottom Line: Intriguingly, rings always form at the cells' anterior edge before migrating towards the center as contractility increases, reflecting a novel dynamical property of the cortex.We develop a simple model of the physical forces underlying this tug-of-war, which quantitatively reproduces our results.We thus propose a quantitative framework for dissecting the relative contribution of contractility and PCP to the self-assembly and repositioning of cytoskeletal structures, which should be applicable to other morphogenetic events.

View Article: PubMed Central - PubMed

Affiliation: Sars International Centre for Marine Molecular Biology, University of Bergen, Bergen, Norway.

ABSTRACT
The actomyosin cytoskeleton is a primary force-generating mechanism in morphogenesis, thus a robust spatial control of cytoskeletal positioning is essential. In this report, we demonstrate that actomyosin contractility and planar cell polarity (PCP) interact in post-mitotic Ciona notochord cells to self-assemble and reposition actomyosin rings, which play an essential role for cell elongation. Intriguingly, rings always form at the cells' anterior edge before migrating towards the center as contractility increases, reflecting a novel dynamical property of the cortex. Our drug and genetic manipulations uncover a tug-of-war between contractility, which localizes cortical flows toward the equator and PCP, which tries to reposition them. We develop a simple model of the physical forces underlying this tug-of-war, which quantitatively reproduces our results. We thus propose a quantitative framework for dissecting the relative contribution of contractility and PCP to the self-assembly and repositioning of cytoskeletal structures, which should be applicable to other morphogenetic events.

No MeSH data available.


Effect of a blebbistatin treatment at time = 110 min on the ring dynamic with .DOI:http://dx.doi.org/10.7554/eLife.09206.025
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fig16: Effect of a blebbistatin treatment at time = 110 min on the ring dynamic with .DOI:http://dx.doi.org/10.7554/eLife.09206.025

Mentions: To mimic the fact that the dynamic of the shift is blebbistatin dose dependent, we assume that after the ring has migrated, contractility decreases to a value . We show on Appendix figure 9 how the dynamic of the shift back to the anterior pole is affected. A more modest impairment of contractility slows down the ring migration velocity as observed experimentally.10.7554/eLife.09206.025Appendix figure 9.Effect of a blebbistatin treatment at time = 110 min on the ring dynamic with .


Assembly and positioning of actomyosin rings by contractility and planar cell polarity.

Sehring IM, Recho P, Denker E, Kourakis M, Mathiesen B, Hannezo E, Dong B, Jiang D - Elife (2015)

Effect of a blebbistatin treatment at time = 110 min on the ring dynamic with .DOI:http://dx.doi.org/10.7554/eLife.09206.025
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4612727&req=5

fig16: Effect of a blebbistatin treatment at time = 110 min on the ring dynamic with .DOI:http://dx.doi.org/10.7554/eLife.09206.025
Mentions: To mimic the fact that the dynamic of the shift is blebbistatin dose dependent, we assume that after the ring has migrated, contractility decreases to a value . We show on Appendix figure 9 how the dynamic of the shift back to the anterior pole is affected. A more modest impairment of contractility slows down the ring migration velocity as observed experimentally.10.7554/eLife.09206.025Appendix figure 9.Effect of a blebbistatin treatment at time = 110 min on the ring dynamic with .

Bottom Line: Intriguingly, rings always form at the cells' anterior edge before migrating towards the center as contractility increases, reflecting a novel dynamical property of the cortex.We develop a simple model of the physical forces underlying this tug-of-war, which quantitatively reproduces our results.We thus propose a quantitative framework for dissecting the relative contribution of contractility and PCP to the self-assembly and repositioning of cytoskeletal structures, which should be applicable to other morphogenetic events.

View Article: PubMed Central - PubMed

Affiliation: Sars International Centre for Marine Molecular Biology, University of Bergen, Bergen, Norway.

ABSTRACT
The actomyosin cytoskeleton is a primary force-generating mechanism in morphogenesis, thus a robust spatial control of cytoskeletal positioning is essential. In this report, we demonstrate that actomyosin contractility and planar cell polarity (PCP) interact in post-mitotic Ciona notochord cells to self-assemble and reposition actomyosin rings, which play an essential role for cell elongation. Intriguingly, rings always form at the cells' anterior edge before migrating towards the center as contractility increases, reflecting a novel dynamical property of the cortex. Our drug and genetic manipulations uncover a tug-of-war between contractility, which localizes cortical flows toward the equator and PCP, which tries to reposition them. We develop a simple model of the physical forces underlying this tug-of-war, which quantitatively reproduces our results. We thus propose a quantitative framework for dissecting the relative contribution of contractility and PCP to the self-assembly and repositioning of cytoskeletal structures, which should be applicable to other morphogenetic events.

No MeSH data available.