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Diversity and distribution of Actinobacteria associated with reef coral Porites lutea.

Kuang W, Li J, Zhang S, Long L - Front Microbiol (2015)

Bottom Line: The Actinobacteria diversity was significantly different between the P. lutea and the surrounding seawater.Analysis of the relationship between actinobacteria assemblages and the environmental parameters showed that several genera were closely related to specific environmental factors.This study highlights that coral-associated actinobacteria populations are highly diverse, and spatially structured within P. lutea, and they are distinct from which in the ambient seawater.

View Article: PubMed Central - PubMed

Affiliation: CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences Guangzhou, China ; College of Earth Science, University of Chinese Academy of Sciences Beijing, China.

ABSTRACT
Actinobacteria is a ubiquitous major group in coral holobiont. The diversity and spatial and temporal distribution of actinobacteria have been rarely documented. In this study, diversity of actinobacteria associated with mucus, tissue and skeleton of Porites lutea and in the surrounding seawater were examined every 3 months for 1 year on Luhuitou fringing reef. The population structures of the P. lutea-associated actinobacteria were analyzed using phylogenetic analysis of 16S rRNA gene clone libraries, which demonstrated highly diverse actinobacteria profiles in P. lutea. A total of 25 described families and 10 unnamed families were determined in the populations, and 12 genera were firstly detected in corals. The Actinobacteria diversity was significantly different between the P. lutea and the surrounding seawater. Only 10 OTUs were shared by the seawater and coral samples. Redundancy and hierarchical cluster analyses were performed to analyze the correlation between the variations of actinobacteria population within the divergent compartments of P. lutea, seasonal changes, and environmental factors. The actinobacteria communities in the same coral compartment tended to cluster together. Even so, an extremely small fraction of OTUs was common in all three P. lutea compartments. Analysis of the relationship between actinobacteria assemblages and the environmental parameters showed that several genera were closely related to specific environmental factors. This study highlights that coral-associated actinobacteria populations are highly diverse, and spatially structured within P. lutea, and they are distinct from which in the ambient seawater.

No MeSH data available.


Rarefaction curves of Actinobacteria 16S rRNA gene sequences.
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Figure 1: Rarefaction curves of Actinobacteria 16S rRNA gene sequences.

Mentions: A total of 2403 sequences were obtained from sixteen 16S rRNA gene clone libraries, resulting in 395 OTUs (stringency at 97%). The rarefaction analysis result showed that most of the curves did not flatten to asymptote, but climbed less steeply (Figure 1). The coverages ranged from 0.69 to 0.97 in 16 libraries, and the average coverage was 0.83 (Table 1). The highest number of OTUs was found in the tissue collected in May, while the lowest OTUs was found in the skeleton collected in November (Table 1). The Shannon indices in mucus collected in different months ranged from 2.32 to 3.44, from 2.45 to 3.55 in tissues, from 1.82 to 3.35 in skeleton, and from 1.53 to 2.82 in sea water (Table 1), and the diversity in the actinobacterial community associated with P. lutea was higher than which in the surrounding sea water (P = 0.045).


Diversity and distribution of Actinobacteria associated with reef coral Porites lutea.

Kuang W, Li J, Zhang S, Long L - Front Microbiol (2015)

Rarefaction curves of Actinobacteria 16S rRNA gene sequences.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4612714&req=5

Figure 1: Rarefaction curves of Actinobacteria 16S rRNA gene sequences.
Mentions: A total of 2403 sequences were obtained from sixteen 16S rRNA gene clone libraries, resulting in 395 OTUs (stringency at 97%). The rarefaction analysis result showed that most of the curves did not flatten to asymptote, but climbed less steeply (Figure 1). The coverages ranged from 0.69 to 0.97 in 16 libraries, and the average coverage was 0.83 (Table 1). The highest number of OTUs was found in the tissue collected in May, while the lowest OTUs was found in the skeleton collected in November (Table 1). The Shannon indices in mucus collected in different months ranged from 2.32 to 3.44, from 2.45 to 3.55 in tissues, from 1.82 to 3.35 in skeleton, and from 1.53 to 2.82 in sea water (Table 1), and the diversity in the actinobacterial community associated with P. lutea was higher than which in the surrounding sea water (P = 0.045).

Bottom Line: The Actinobacteria diversity was significantly different between the P. lutea and the surrounding seawater.Analysis of the relationship between actinobacteria assemblages and the environmental parameters showed that several genera were closely related to specific environmental factors.This study highlights that coral-associated actinobacteria populations are highly diverse, and spatially structured within P. lutea, and they are distinct from which in the ambient seawater.

View Article: PubMed Central - PubMed

Affiliation: CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences Guangzhou, China ; College of Earth Science, University of Chinese Academy of Sciences Beijing, China.

ABSTRACT
Actinobacteria is a ubiquitous major group in coral holobiont. The diversity and spatial and temporal distribution of actinobacteria have been rarely documented. In this study, diversity of actinobacteria associated with mucus, tissue and skeleton of Porites lutea and in the surrounding seawater were examined every 3 months for 1 year on Luhuitou fringing reef. The population structures of the P. lutea-associated actinobacteria were analyzed using phylogenetic analysis of 16S rRNA gene clone libraries, which demonstrated highly diverse actinobacteria profiles in P. lutea. A total of 25 described families and 10 unnamed families were determined in the populations, and 12 genera were firstly detected in corals. The Actinobacteria diversity was significantly different between the P. lutea and the surrounding seawater. Only 10 OTUs were shared by the seawater and coral samples. Redundancy and hierarchical cluster analyses were performed to analyze the correlation between the variations of actinobacteria population within the divergent compartments of P. lutea, seasonal changes, and environmental factors. The actinobacteria communities in the same coral compartment tended to cluster together. Even so, an extremely small fraction of OTUs was common in all three P. lutea compartments. Analysis of the relationship between actinobacteria assemblages and the environmental parameters showed that several genera were closely related to specific environmental factors. This study highlights that coral-associated actinobacteria populations are highly diverse, and spatially structured within P. lutea, and they are distinct from which in the ambient seawater.

No MeSH data available.