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In vivo differences in inputs and spiking between neurons in lobules VI/VII of neocerebellum and lobule X of archaeocerebellum.

Witter L, De Zeeuw CI - Cerebellum (2015)

Bottom Line: Using whole-cell and cell-attached recordings in vivo in anesthetized mice, we show that the mossy fiber inputs to these functionally distinct areas of the cerebellum differ in that the irregularity and bursty character of their firing is significantly greater in lobules VI/VII than in lobule X.Importantly, this difference in mossy fiber regularity is propagated through the granule cells at the input stage to the Purkinje cells and molecular layer interneurons, ultimately resulting in different regularity of simple spikes.These data show that the firing behavior of cerebellar cortical neurons does not only reflect particular intrinsic properties but also an interesting interplay with the innate activity at the input stage.

View Article: PubMed Central - PubMed

Affiliation: Netherlands Institute for Neuroscience, Royal Academy for Arts and Sciences (KNAW), Meibergdreef 47, 1105 BA, Amsterdam, The Netherlands.

ABSTRACT
The cerebellum plays an important role in the coordination and refinement of movements and cognitive processes. Recently, it has been shown that the main output neuron of the cerebellar cortex, i.e., the Purkinje cell, can show a different firing behavior dependent on its intrinsic electrophysiological properties. Yet, to what extent a different nature of mossy fiber inputs can influence the firing behavior of cerebellar cortical neurons remains to be elucidated. Here, we compared the firing rate and regularity of mossy fibers and neurons in two different regions of cerebellar cortex. One region intimately connected with the cerebral cortex, i.e., lobules VI/VII of the neocerebellum, and another one strongly connected with the vestibular apparatus, i.e., lobule X of the archaeocerebellum. Given their connections, we hypothesized that activity in neurons in lobules VI/VII and lobule X may be expected to be more phasic and tonic, respectively. Using whole-cell and cell-attached recordings in vivo in anesthetized mice, we show that the mossy fiber inputs to these functionally distinct areas of the cerebellum differ in that the irregularity and bursty character of their firing is significantly greater in lobules VI/VII than in lobule X. Importantly, this difference in mossy fiber regularity is propagated through the granule cells at the input stage to the Purkinje cells and molecular layer interneurons, ultimately resulting in different regularity of simple spikes. These data show that the firing behavior of cerebellar cortical neurons does not only reflect particular intrinsic properties but also an interesting interplay with the innate activity at the input stage.

No MeSH data available.


Reconstructions of recorded neurons. a Granule cell. The soma is approximately 8 μm across. The ascending branch of the parallel fiber can be observed leaving the soma at the 1 o’clock position. Two dendrites can be observed at the 6 and 10 o’clock positions. b Purkinje cell. The typical large soma and dendritic tree were reconstructed. c A basket cell was reconstructed. The axon and dendrites could not be readily distinguished and are both shown in black. Outlines indicate the Purkinje cell layer. d Confocal z-stack projection of a recovered unipolar brush cell. The typical brush-like dendrite can be seen at the left of the cell body, while the axon can be seen leaving the cell at the right
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Fig1: Reconstructions of recorded neurons. a Granule cell. The soma is approximately 8 μm across. The ascending branch of the parallel fiber can be observed leaving the soma at the 1 o’clock position. Two dendrites can be observed at the 6 and 10 o’clock positions. b Purkinje cell. The typical large soma and dendritic tree were reconstructed. c A basket cell was reconstructed. The axon and dendrites could not be readily distinguished and are both shown in black. Outlines indicate the Purkinje cell layer. d Confocal z-stack projection of a recovered unipolar brush cell. The typical brush-like dendrite can be seen at the left of the cell body, while the axon can be seen leaving the cell at the right

Mentions: Whole cell in vivo recordings were made from neurons in lobules VI/VII and lobule X of the cerebellar cortex (Figs. 1 and 2a). There was no difference in the success rate of obtaining a patch between these areas. Each cerebellar cortical cell type showed characteristic suprathreshold and subthreshold activities and characteristic responses to current input. We used such activities and input–output responses to identify every neuron type and verified this with morphological identification [21, 22].Fig. 1


In vivo differences in inputs and spiking between neurons in lobules VI/VII of neocerebellum and lobule X of archaeocerebellum.

Witter L, De Zeeuw CI - Cerebellum (2015)

Reconstructions of recorded neurons. a Granule cell. The soma is approximately 8 μm across. The ascending branch of the parallel fiber can be observed leaving the soma at the 1 o’clock position. Two dendrites can be observed at the 6 and 10 o’clock positions. b Purkinje cell. The typical large soma and dendritic tree were reconstructed. c A basket cell was reconstructed. The axon and dendrites could not be readily distinguished and are both shown in black. Outlines indicate the Purkinje cell layer. d Confocal z-stack projection of a recovered unipolar brush cell. The typical brush-like dendrite can be seen at the left of the cell body, while the axon can be seen leaving the cell at the right
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4612334&req=5

Fig1: Reconstructions of recorded neurons. a Granule cell. The soma is approximately 8 μm across. The ascending branch of the parallel fiber can be observed leaving the soma at the 1 o’clock position. Two dendrites can be observed at the 6 and 10 o’clock positions. b Purkinje cell. The typical large soma and dendritic tree were reconstructed. c A basket cell was reconstructed. The axon and dendrites could not be readily distinguished and are both shown in black. Outlines indicate the Purkinje cell layer. d Confocal z-stack projection of a recovered unipolar brush cell. The typical brush-like dendrite can be seen at the left of the cell body, while the axon can be seen leaving the cell at the right
Mentions: Whole cell in vivo recordings were made from neurons in lobules VI/VII and lobule X of the cerebellar cortex (Figs. 1 and 2a). There was no difference in the success rate of obtaining a patch between these areas. Each cerebellar cortical cell type showed characteristic suprathreshold and subthreshold activities and characteristic responses to current input. We used such activities and input–output responses to identify every neuron type and verified this with morphological identification [21, 22].Fig. 1

Bottom Line: Using whole-cell and cell-attached recordings in vivo in anesthetized mice, we show that the mossy fiber inputs to these functionally distinct areas of the cerebellum differ in that the irregularity and bursty character of their firing is significantly greater in lobules VI/VII than in lobule X.Importantly, this difference in mossy fiber regularity is propagated through the granule cells at the input stage to the Purkinje cells and molecular layer interneurons, ultimately resulting in different regularity of simple spikes.These data show that the firing behavior of cerebellar cortical neurons does not only reflect particular intrinsic properties but also an interesting interplay with the innate activity at the input stage.

View Article: PubMed Central - PubMed

Affiliation: Netherlands Institute for Neuroscience, Royal Academy for Arts and Sciences (KNAW), Meibergdreef 47, 1105 BA, Amsterdam, The Netherlands.

ABSTRACT
The cerebellum plays an important role in the coordination and refinement of movements and cognitive processes. Recently, it has been shown that the main output neuron of the cerebellar cortex, i.e., the Purkinje cell, can show a different firing behavior dependent on its intrinsic electrophysiological properties. Yet, to what extent a different nature of mossy fiber inputs can influence the firing behavior of cerebellar cortical neurons remains to be elucidated. Here, we compared the firing rate and regularity of mossy fibers and neurons in two different regions of cerebellar cortex. One region intimately connected with the cerebral cortex, i.e., lobules VI/VII of the neocerebellum, and another one strongly connected with the vestibular apparatus, i.e., lobule X of the archaeocerebellum. Given their connections, we hypothesized that activity in neurons in lobules VI/VII and lobule X may be expected to be more phasic and tonic, respectively. Using whole-cell and cell-attached recordings in vivo in anesthetized mice, we show that the mossy fiber inputs to these functionally distinct areas of the cerebellum differ in that the irregularity and bursty character of their firing is significantly greater in lobules VI/VII than in lobule X. Importantly, this difference in mossy fiber regularity is propagated through the granule cells at the input stage to the Purkinje cells and molecular layer interneurons, ultimately resulting in different regularity of simple spikes. These data show that the firing behavior of cerebellar cortical neurons does not only reflect particular intrinsic properties but also an interesting interplay with the innate activity at the input stage.

No MeSH data available.