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Visuo-vestibular information processing by unipolar brush cells in the rabbit flocculus.

Hensbroek RA, Ruigrok TJ, van Beugen BJ, Maruta J, Simpson JI - Cerebellum (2015)

Bottom Line: The unipolar brush cell (UBC) is a glutamatergic granular layer interneuron that is predominantly located in the vestibulocerebellum and parts of the vermis.In the anesthetized rabbit, the activity of the presumed UBC units displayed a wide variety of modulation profiles that could be related to aspects of head velocity or acceleration.These modulation profiles could also be found in the awake rabbit where, in addition, they could also carry an eye position signal.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuroscience & Physiology, New York University Medical School, New York, NY, 10016, USA.

ABSTRACT
The unipolar brush cell (UBC) is a glutamatergic granular layer interneuron that is predominantly located in the vestibulocerebellum and parts of the vermis. In rat and rabbit, we previously found using juxtacellular labeling combined with spontaneous activity recording that cells with highly regular spontaneous activity belong to the UBC category. Making use of this signature, we recorded from floccular UBCs in both anesthetized and awake rabbits while delivering visuo-vestibular stimulation by using sigmoidal rotation of the whole animal. In the anesthetized rabbit, the activity of the presumed UBC units displayed a wide variety of modulation profiles that could be related to aspects of head velocity or acceleration. These modulation profiles could also be found in the awake rabbit where, in addition, they could also carry an eye position signal. Furthermore, units in the awake rabbit could demonstrate rather long response latencies of up to 0.5 s. We suggest that the UBCs recorded in this study mostly belong to the type I UBC category (calretinin-positive) and that they can play diverse roles in floccular visuo-vestibular information processing, such as transformation of velocity-related signals to acceleration-related signals.

No MeSH data available.


Related in: MedlinePlus

Response properties of floccular UBCs in the anesthetized rabbit. a Response kinematics of sigmoidal rotation. Top trace, head position; middle trace, head velocity, monophasic for each direction of rotation; bottom trace, head acceleration, biphasic for each direction of rotation. The dashed vertical lines indicate the peak velocity for each rotation direction. The solid vertical lines indicate the start and stop of the sigmoidal movement. b Example (unit 12.4d.1) demonstrating the narrow interspike interval (ISI) distribution. Top left panel, ISI histogram showing spike intervals during spontaneous activity expressed as normalized counts; top right panel, sequential log interspike intervals over a period of 30 s of spontaneous activity; middle panel, head position during sigmoidal stimulation, which was provided in the light by movement of the turntable by hand. The table movement provided by hand had a mean peak velocity and standard deviation of 64.5 ± 3.6° per second for contralateral movement and 67.9 ± 6.7° per second for ipsilateral movement; bottom panel, modulation profile (average of 7 cycles) in response to the corresponding head movement shown in the middle panel. The 0 reference in the time line indicates the onset of the turntable movement. Note the similarity of the modulation profile to the velocity trace in Fig. 1a. c Example (unit 7.1a.2) with a lower, but also regular firing pattern. Panels are arranged as in (b). The response to head movement was averaged over 16 cycles. The table movement provided by hand had a mean peak velocity and standard deviation of 42.8 ± 3.8° per second for contralateral movement and 41.0 ± 6.0° per second for ipsilateral movement. Note that the resulting modulation profile resembles the head acceleration profile, although shifted and spread out in time
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Fig1: Response properties of floccular UBCs in the anesthetized rabbit. a Response kinematics of sigmoidal rotation. Top trace, head position; middle trace, head velocity, monophasic for each direction of rotation; bottom trace, head acceleration, biphasic for each direction of rotation. The dashed vertical lines indicate the peak velocity for each rotation direction. The solid vertical lines indicate the start and stop of the sigmoidal movement. b Example (unit 12.4d.1) demonstrating the narrow interspike interval (ISI) distribution. Top left panel, ISI histogram showing spike intervals during spontaneous activity expressed as normalized counts; top right panel, sequential log interspike intervals over a period of 30 s of spontaneous activity; middle panel, head position during sigmoidal stimulation, which was provided in the light by movement of the turntable by hand. The table movement provided by hand had a mean peak velocity and standard deviation of 64.5 ± 3.6° per second for contralateral movement and 67.9 ± 6.7° per second for ipsilateral movement; bottom panel, modulation profile (average of 7 cycles) in response to the corresponding head movement shown in the middle panel. The 0 reference in the time line indicates the onset of the turntable movement. Note the similarity of the modulation profile to the velocity trace in Fig. 1a. c Example (unit 7.1a.2) with a lower, but also regular firing pattern. Panels are arranged as in (b). The response to head movement was averaged over 16 cycles. The table movement provided by hand had a mean peak velocity and standard deviation of 42.8 ± 3.8° per second for contralateral movement and 41.0 ± 6.0° per second for ipsilateral movement. Note that the resulting modulation profile resembles the head acceleration profile, although shifted and spread out in time

Mentions: Units identified as UBCs were recorded during visual-vestibular stimulation in the light and dark using sigmoidal rotation provided by a turntable. Sigmoidal stimulation mimics natural head movement [16] with a monophasic velocity profile and a biphasic acceleration profile (Fig. 1a). Compared to sinusoidal stimulation, sigmoidal stimulation allows for a clearer separation among position-, velocity-, and acceleration-related responses and thus provides improved detection of response asymmetries and comparison to the level of spontaneous activity.Fig. 1


Visuo-vestibular information processing by unipolar brush cells in the rabbit flocculus.

Hensbroek RA, Ruigrok TJ, van Beugen BJ, Maruta J, Simpson JI - Cerebellum (2015)

Response properties of floccular UBCs in the anesthetized rabbit. a Response kinematics of sigmoidal rotation. Top trace, head position; middle trace, head velocity, monophasic for each direction of rotation; bottom trace, head acceleration, biphasic for each direction of rotation. The dashed vertical lines indicate the peak velocity for each rotation direction. The solid vertical lines indicate the start and stop of the sigmoidal movement. b Example (unit 12.4d.1) demonstrating the narrow interspike interval (ISI) distribution. Top left panel, ISI histogram showing spike intervals during spontaneous activity expressed as normalized counts; top right panel, sequential log interspike intervals over a period of 30 s of spontaneous activity; middle panel, head position during sigmoidal stimulation, which was provided in the light by movement of the turntable by hand. The table movement provided by hand had a mean peak velocity and standard deviation of 64.5 ± 3.6° per second for contralateral movement and 67.9 ± 6.7° per second for ipsilateral movement; bottom panel, modulation profile (average of 7 cycles) in response to the corresponding head movement shown in the middle panel. The 0 reference in the time line indicates the onset of the turntable movement. Note the similarity of the modulation profile to the velocity trace in Fig. 1a. c Example (unit 7.1a.2) with a lower, but also regular firing pattern. Panels are arranged as in (b). The response to head movement was averaged over 16 cycles. The table movement provided by hand had a mean peak velocity and standard deviation of 42.8 ± 3.8° per second for contralateral movement and 41.0 ± 6.0° per second for ipsilateral movement. Note that the resulting modulation profile resembles the head acceleration profile, although shifted and spread out in time
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Related In: Results  -  Collection

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Fig1: Response properties of floccular UBCs in the anesthetized rabbit. a Response kinematics of sigmoidal rotation. Top trace, head position; middle trace, head velocity, monophasic for each direction of rotation; bottom trace, head acceleration, biphasic for each direction of rotation. The dashed vertical lines indicate the peak velocity for each rotation direction. The solid vertical lines indicate the start and stop of the sigmoidal movement. b Example (unit 12.4d.1) demonstrating the narrow interspike interval (ISI) distribution. Top left panel, ISI histogram showing spike intervals during spontaneous activity expressed as normalized counts; top right panel, sequential log interspike intervals over a period of 30 s of spontaneous activity; middle panel, head position during sigmoidal stimulation, which was provided in the light by movement of the turntable by hand. The table movement provided by hand had a mean peak velocity and standard deviation of 64.5 ± 3.6° per second for contralateral movement and 67.9 ± 6.7° per second for ipsilateral movement; bottom panel, modulation profile (average of 7 cycles) in response to the corresponding head movement shown in the middle panel. The 0 reference in the time line indicates the onset of the turntable movement. Note the similarity of the modulation profile to the velocity trace in Fig. 1a. c Example (unit 7.1a.2) with a lower, but also regular firing pattern. Panels are arranged as in (b). The response to head movement was averaged over 16 cycles. The table movement provided by hand had a mean peak velocity and standard deviation of 42.8 ± 3.8° per second for contralateral movement and 41.0 ± 6.0° per second for ipsilateral movement. Note that the resulting modulation profile resembles the head acceleration profile, although shifted and spread out in time
Mentions: Units identified as UBCs were recorded during visual-vestibular stimulation in the light and dark using sigmoidal rotation provided by a turntable. Sigmoidal stimulation mimics natural head movement [16] with a monophasic velocity profile and a biphasic acceleration profile (Fig. 1a). Compared to sinusoidal stimulation, sigmoidal stimulation allows for a clearer separation among position-, velocity-, and acceleration-related responses and thus provides improved detection of response asymmetries and comparison to the level of spontaneous activity.Fig. 1

Bottom Line: The unipolar brush cell (UBC) is a glutamatergic granular layer interneuron that is predominantly located in the vestibulocerebellum and parts of the vermis.In the anesthetized rabbit, the activity of the presumed UBC units displayed a wide variety of modulation profiles that could be related to aspects of head velocity or acceleration.These modulation profiles could also be found in the awake rabbit where, in addition, they could also carry an eye position signal.

View Article: PubMed Central - PubMed

Affiliation: Department of Neuroscience & Physiology, New York University Medical School, New York, NY, 10016, USA.

ABSTRACT
The unipolar brush cell (UBC) is a glutamatergic granular layer interneuron that is predominantly located in the vestibulocerebellum and parts of the vermis. In rat and rabbit, we previously found using juxtacellular labeling combined with spontaneous activity recording that cells with highly regular spontaneous activity belong to the UBC category. Making use of this signature, we recorded from floccular UBCs in both anesthetized and awake rabbits while delivering visuo-vestibular stimulation by using sigmoidal rotation of the whole animal. In the anesthetized rabbit, the activity of the presumed UBC units displayed a wide variety of modulation profiles that could be related to aspects of head velocity or acceleration. These modulation profiles could also be found in the awake rabbit where, in addition, they could also carry an eye position signal. Furthermore, units in the awake rabbit could demonstrate rather long response latencies of up to 0.5 s. We suggest that the UBCs recorded in this study mostly belong to the type I UBC category (calretinin-positive) and that they can play diverse roles in floccular visuo-vestibular information processing, such as transformation of velocity-related signals to acceleration-related signals.

No MeSH data available.


Related in: MedlinePlus