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Effects of salinity on the growth, physiology and relevant gene expression of an annual halophyte grown from heteromorphic seeds.

Cao J, Lv XY, Chen L, Xing JJ, Lan HY - AoB Plants (2015)

Bottom Line: Results showed that osmolytes (proline and glycinebetaine) were significantly increased and that excess reactive oxygen species ([Formula: see text] H2O2) produced under high salinity were scavenged by increased levels of antioxidant enzymes (superoxide dismutase, ascorbate peroxidase and glutathione reductase) and corresponding antioxidants (ascorbic acid and glutathione).Moreover, enhancement of phosphoenolpyruvate carboxylase activity at high salt intensity had a positive effect on photosynthesis.In conclusion, we found that high salinity induced the same active physiological responses in plants from heteromorphic seeds of S. aralocaspica, there was no carry-over of seed heteromorphism to plants: all the descendants required salinity for optimal growth and adaptation to their natural habitat.

View Article: PubMed Central - PubMed

Affiliation: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.

No MeSH data available.


qRT-PCR analysis of gene expression pattern on ion transportation and osmolyte synthesis in leaves during long-term NaCl treatment. (A) V-PPase, (B) V-ATPase, (C) pyrroline-5-carboxylate synthase gene and (D) BADH gene. β-actin as internal reference. F-values are given when significance levels are reached (S, salt; ST, seed type; *P< 0.05, **P< 0.01, ***P< 0.001). Bars with different uppercase letters indicate significant differences (P< 0.05) according to Tukey's test. Values are means ± SE of six replicates (including three biological replicates and two technical replicates). Bl, black seed; Br, brown seed.
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PLV112F4: qRT-PCR analysis of gene expression pattern on ion transportation and osmolyte synthesis in leaves during long-term NaCl treatment. (A) V-PPase, (B) V-ATPase, (C) pyrroline-5-carboxylate synthase gene and (D) BADH gene. β-actin as internal reference. F-values are given when significance levels are reached (S, salt; ST, seed type; *P< 0.05, **P< 0.01, ***P< 0.001). Bars with different uppercase letters indicate significant differences (P< 0.05) according to Tukey's test. Values are means ± SE of six replicates (including three biological replicates and two technical replicates). Bl, black seed; Br, brown seed.

Mentions: The expression level of vacuolar pyrophosphatase gene (V-PPase) was significantly increased under 300 and 500 mM NaCl treatment in plants from brown and black seeds (F3,40 = 22.32, P< 0.0001) (Fig. 4A), while that of the key subunit B of vacuolar ATP synthase gene (V-ATPase) remained unchanged except for the plants from black seeds under 300 mM NaCl treatment (F3,40 = 130.0, P< 0.0001) (Fig. 4B). In comparison between the plants derived from the two types of seeds, an apparent difference was observed in the transcript accumulation of V-PPase (F1,40 = 43.95, P< 0.0001) and V-ATPase (F1,40 = 101.0, P< 0.0001) at 300 mM NaCl treatment: expression was significantly higher in plants from black seed compared with that of plants from brown seed (t10 = 11.40, P< 0.0001 for V-PPase; t10 = 29.65, P< 0.0001 for V-ATPase).Figure 4.


Effects of salinity on the growth, physiology and relevant gene expression of an annual halophyte grown from heteromorphic seeds.

Cao J, Lv XY, Chen L, Xing JJ, Lan HY - AoB Plants (2015)

qRT-PCR analysis of gene expression pattern on ion transportation and osmolyte synthesis in leaves during long-term NaCl treatment. (A) V-PPase, (B) V-ATPase, (C) pyrroline-5-carboxylate synthase gene and (D) BADH gene. β-actin as internal reference. F-values are given when significance levels are reached (S, salt; ST, seed type; *P< 0.05, **P< 0.01, ***P< 0.001). Bars with different uppercase letters indicate significant differences (P< 0.05) according to Tukey's test. Values are means ± SE of six replicates (including three biological replicates and two technical replicates). Bl, black seed; Br, brown seed.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4612296&req=5

PLV112F4: qRT-PCR analysis of gene expression pattern on ion transportation and osmolyte synthesis in leaves during long-term NaCl treatment. (A) V-PPase, (B) V-ATPase, (C) pyrroline-5-carboxylate synthase gene and (D) BADH gene. β-actin as internal reference. F-values are given when significance levels are reached (S, salt; ST, seed type; *P< 0.05, **P< 0.01, ***P< 0.001). Bars with different uppercase letters indicate significant differences (P< 0.05) according to Tukey's test. Values are means ± SE of six replicates (including three biological replicates and two technical replicates). Bl, black seed; Br, brown seed.
Mentions: The expression level of vacuolar pyrophosphatase gene (V-PPase) was significantly increased under 300 and 500 mM NaCl treatment in plants from brown and black seeds (F3,40 = 22.32, P< 0.0001) (Fig. 4A), while that of the key subunit B of vacuolar ATP synthase gene (V-ATPase) remained unchanged except for the plants from black seeds under 300 mM NaCl treatment (F3,40 = 130.0, P< 0.0001) (Fig. 4B). In comparison between the plants derived from the two types of seeds, an apparent difference was observed in the transcript accumulation of V-PPase (F1,40 = 43.95, P< 0.0001) and V-ATPase (F1,40 = 101.0, P< 0.0001) at 300 mM NaCl treatment: expression was significantly higher in plants from black seed compared with that of plants from brown seed (t10 = 11.40, P< 0.0001 for V-PPase; t10 = 29.65, P< 0.0001 for V-ATPase).Figure 4.

Bottom Line: Results showed that osmolytes (proline and glycinebetaine) were significantly increased and that excess reactive oxygen species ([Formula: see text] H2O2) produced under high salinity were scavenged by increased levels of antioxidant enzymes (superoxide dismutase, ascorbate peroxidase and glutathione reductase) and corresponding antioxidants (ascorbic acid and glutathione).Moreover, enhancement of phosphoenolpyruvate carboxylase activity at high salt intensity had a positive effect on photosynthesis.In conclusion, we found that high salinity induced the same active physiological responses in plants from heteromorphic seeds of S. aralocaspica, there was no carry-over of seed heteromorphism to plants: all the descendants required salinity for optimal growth and adaptation to their natural habitat.

View Article: PubMed Central - PubMed

Affiliation: Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China.

No MeSH data available.