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Increased Cytotoxicity of Vanadium to CHO-K1 Cells in the Presence of Inorganic Selenium.

Zwolak I - Bull Environ Contam Toxicol (2015)

Bottom Line: The effect of selenium applied as sodium selenite (Na2SeO3) on the cytotoxicity of vanadyl sulphate (VOSO4) was examined using CHO-K1 cells.Observations performed with a phase-contrast microscope showed most cells to be rounded upon treatment with VOSO4 alone.In turn, a majority of cells co-treated with VOSO4 and 1 μM Na2SeO3 were elongated, and exhibited cytoplasmic vacuolization.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology, Institute of Environmental Protection, The John Paul II Catholic University of Lublin, Kraśnicka Ave. 102, 20-718, Lublin, Poland. iglina@kul.lublin.pl.

ABSTRACT
The effect of selenium applied as sodium selenite (Na2SeO3) on the cytotoxicity of vanadyl sulphate (VOSO4) was examined using CHO-K1 cells. From the resazurin-based assay, it appears that Na2SeO3 at low doses (0.5 and 1 μM) can enhance 100 μM VOSO4-induced cell damage. The two-way ANOVA analysis revealed that the increased cell damage was a consequence of a synergistic interaction of 0.5 μM Na2SeO3 with VOSO4 and 1 μM Na2SeO3 with VOSO4. Observations performed with a phase-contrast microscope showed most cells to be rounded upon treatment with VOSO4 alone. In turn, a majority of cells co-treated with VOSO4 and 1 μM Na2SeO3 were elongated, and exhibited cytoplasmic vacuolization. These results warn of the potential contribution of inorganic selenium to vanadium-induced toxicity.

No MeSH data available.


Related in: MedlinePlus

Morphology of CHO-K1 cells under the phase-contrast microscope. a Control cell culture, b cells after 48-h exposure to 100 μM VOSO4, c cells after 48-h exposure to the combination of 100 μM VOSO4 with 1 μM Na2SeO3
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Fig2: Morphology of CHO-K1 cells under the phase-contrast microscope. a Control cell culture, b cells after 48-h exposure to 100 μM VOSO4, c cells after 48-h exposure to the combination of 100 μM VOSO4 with 1 μM Na2SeO3

Mentions: The analysis of cell morphology carried out under the phase-contrast microscope showed that the control CHO-K1 cell culture (at 72 h from seeding) developed a dense monolayer of cells with an epithelial-like morphology (Fig. 2a). The 48 h treatment with either of the selenite concentrations tested did not induce any changes in the appearance of the cells, compared to the untreated control cells (data not shown). Conversely, as expected, 100 μM vanadyl promoted significant changes in the appearance of the cells, compared to the control. In this treatment group, most cells were rounded and some cells were shrunk or spindle shaped (Fig. 2b). Additionally, nuclear chromatin condensation and marginalization was observed in the vanadyl-treated cells. As shown in Fig. 2c, cells exposed simultaneously to vanadyl and 1 μM selenite for 48 h obviously differed in their morphology from the vanadyl-only treated cells. Most cells were spread and elongated in shape and exhibited cytoplasmic vacuolization (Fig. 2c). The above-described changes were also observed in cultures treated with vanadyl and 0.5 μM selenite, but they were less intense. The morphology of the cells incubated with vanadyl and 0.1 μM selenite was comparable to that of the vanadyl-only treated cells (data not shown).Fig. 2


Increased Cytotoxicity of Vanadium to CHO-K1 Cells in the Presence of Inorganic Selenium.

Zwolak I - Bull Environ Contam Toxicol (2015)

Morphology of CHO-K1 cells under the phase-contrast microscope. a Control cell culture, b cells after 48-h exposure to 100 μM VOSO4, c cells after 48-h exposure to the combination of 100 μM VOSO4 with 1 μM Na2SeO3
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4608973&req=5

Fig2: Morphology of CHO-K1 cells under the phase-contrast microscope. a Control cell culture, b cells after 48-h exposure to 100 μM VOSO4, c cells after 48-h exposure to the combination of 100 μM VOSO4 with 1 μM Na2SeO3
Mentions: The analysis of cell morphology carried out under the phase-contrast microscope showed that the control CHO-K1 cell culture (at 72 h from seeding) developed a dense monolayer of cells with an epithelial-like morphology (Fig. 2a). The 48 h treatment with either of the selenite concentrations tested did not induce any changes in the appearance of the cells, compared to the untreated control cells (data not shown). Conversely, as expected, 100 μM vanadyl promoted significant changes in the appearance of the cells, compared to the control. In this treatment group, most cells were rounded and some cells were shrunk or spindle shaped (Fig. 2b). Additionally, nuclear chromatin condensation and marginalization was observed in the vanadyl-treated cells. As shown in Fig. 2c, cells exposed simultaneously to vanadyl and 1 μM selenite for 48 h obviously differed in their morphology from the vanadyl-only treated cells. Most cells were spread and elongated in shape and exhibited cytoplasmic vacuolization (Fig. 2c). The above-described changes were also observed in cultures treated with vanadyl and 0.5 μM selenite, but they were less intense. The morphology of the cells incubated with vanadyl and 0.1 μM selenite was comparable to that of the vanadyl-only treated cells (data not shown).Fig. 2

Bottom Line: The effect of selenium applied as sodium selenite (Na2SeO3) on the cytotoxicity of vanadyl sulphate (VOSO4) was examined using CHO-K1 cells.Observations performed with a phase-contrast microscope showed most cells to be rounded upon treatment with VOSO4 alone.In turn, a majority of cells co-treated with VOSO4 and 1 μM Na2SeO3 were elongated, and exhibited cytoplasmic vacuolization.

View Article: PubMed Central - PubMed

Affiliation: Department of Cell Biology, Institute of Environmental Protection, The John Paul II Catholic University of Lublin, Kraśnicka Ave. 102, 20-718, Lublin, Poland. iglina@kul.lublin.pl.

ABSTRACT
The effect of selenium applied as sodium selenite (Na2SeO3) on the cytotoxicity of vanadyl sulphate (VOSO4) was examined using CHO-K1 cells. From the resazurin-based assay, it appears that Na2SeO3 at low doses (0.5 and 1 μM) can enhance 100 μM VOSO4-induced cell damage. The two-way ANOVA analysis revealed that the increased cell damage was a consequence of a synergistic interaction of 0.5 μM Na2SeO3 with VOSO4 and 1 μM Na2SeO3 with VOSO4. Observations performed with a phase-contrast microscope showed most cells to be rounded upon treatment with VOSO4 alone. In turn, a majority of cells co-treated with VOSO4 and 1 μM Na2SeO3 were elongated, and exhibited cytoplasmic vacuolization. These results warn of the potential contribution of inorganic selenium to vanadium-induced toxicity.

No MeSH data available.


Related in: MedlinePlus