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In situ prebiotics: enzymatic release of galacto-rhamnogalacturonan from potato pulp in vivo in the gastrointestinal tract of the weaning piglet.

Strube ML, Jensen TK, Meyer AS, Boye M - AMB Express (2015)

Bottom Line: Using purified prebiotics may however not be cost-effective in the livestock production industry.Instead, prebiotic fibres may be released directly in the gastro-intestinal tract by feeding enzymes with a suitable substrate and allowing the prebiotics to be produced in situ.To our knowledge, this is the first paper describing targeted production of prebiotics in an animal model.

View Article: PubMed Central - PubMed

Affiliation: National Veterinary Institute, Technical University of Denmark, Frederiksberg, Denmark. milst@vet.dtu.dk.

ABSTRACT
Prebiotics may be efficient for prevention of intestinal infections in humans and animals by increasing the levels of beneficial bacteria and thereby improving gut health. Using purified prebiotics may however not be cost-effective in the livestock production industry. Instead, prebiotic fibres may be released directly in the gastro-intestinal tract by feeding enzymes with a suitable substrate and allowing the prebiotics to be produced in situ. Using low doses, 0.03 % enzyme-to-substrate ratio, of the enzymes pectin lyase and polygalacturonase in combination with potato pulp, a low-value industrial by-product, we show that high molecular weight galacto-rhamnogalacturonan can be solubilized in the stomach of weaning piglets. The release of this fiber is in the order of 22-38 % of the theoretical amount, achieved within 20 min. The catalysis takes place mainly in the stomach of the animal and is then followed by distribution through the small intestines. To our knowledge, this is the first paper describing targeted production of prebiotics in an animal model.

No MeSH data available.


Related in: MedlinePlus

Matrix plot of variables chemically associated with galacto-rhamnogalacturonan, e.g. galactose, galacturonic acid, rhamnose and CH100. All collected samples are included, e.g. from all sections at all time-points for both enzyme- and control-treated animals. Each point is the total mass of the given variable in the given sample. r2 is the squared Pearson correlation of the two variables in each plot. CH100 carbohydrate with a molecular mass larger than 100 kDa
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Fig1: Matrix plot of variables chemically associated with galacto-rhamnogalacturonan, e.g. galactose, galacturonic acid, rhamnose and CH100. All collected samples are included, e.g. from all sections at all time-points for both enzyme- and control-treated animals. Each point is the total mass of the given variable in the given sample. r2 is the squared Pearson correlation of the two variables in each plot. CH100 carbohydrate with a molecular mass larger than 100 kDa

Mentions: The mass of released CH100, galactose, galacturonic acid and rhamnose was highly correlated in the animals fed enzyme (Fig. 1); suggesting that released carbohydrate was GRG from potato pulp, as this domain is large and composed of a backbone of rhamnose and galacturonic acid highly substituted by galactose. GRG is from here on used as an umbrella term for these four parameters. This correlations was not found in the control animals, mainly because these samples were almost entirely devoid of both rhamnose and galacturonic acid. A substantial amount of galactose and CH100 was, however, found in the ileal samples of some control animals, but this is presumably of mucosal origin since this was accompanied by high levels of the mucosal carbohydrates galactosamine, glucosamine and fucose.Fig. 1


In situ prebiotics: enzymatic release of galacto-rhamnogalacturonan from potato pulp in vivo in the gastrointestinal tract of the weaning piglet.

Strube ML, Jensen TK, Meyer AS, Boye M - AMB Express (2015)

Matrix plot of variables chemically associated with galacto-rhamnogalacturonan, e.g. galactose, galacturonic acid, rhamnose and CH100. All collected samples are included, e.g. from all sections at all time-points for both enzyme- and control-treated animals. Each point is the total mass of the given variable in the given sample. r2 is the squared Pearson correlation of the two variables in each plot. CH100 carbohydrate with a molecular mass larger than 100 kDa
© Copyright Policy - OpenAccess
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4608949&req=5

Fig1: Matrix plot of variables chemically associated with galacto-rhamnogalacturonan, e.g. galactose, galacturonic acid, rhamnose and CH100. All collected samples are included, e.g. from all sections at all time-points for both enzyme- and control-treated animals. Each point is the total mass of the given variable in the given sample. r2 is the squared Pearson correlation of the two variables in each plot. CH100 carbohydrate with a molecular mass larger than 100 kDa
Mentions: The mass of released CH100, galactose, galacturonic acid and rhamnose was highly correlated in the animals fed enzyme (Fig. 1); suggesting that released carbohydrate was GRG from potato pulp, as this domain is large and composed of a backbone of rhamnose and galacturonic acid highly substituted by galactose. GRG is from here on used as an umbrella term for these four parameters. This correlations was not found in the control animals, mainly because these samples were almost entirely devoid of both rhamnose and galacturonic acid. A substantial amount of galactose and CH100 was, however, found in the ileal samples of some control animals, but this is presumably of mucosal origin since this was accompanied by high levels of the mucosal carbohydrates galactosamine, glucosamine and fucose.Fig. 1

Bottom Line: Using purified prebiotics may however not be cost-effective in the livestock production industry.Instead, prebiotic fibres may be released directly in the gastro-intestinal tract by feeding enzymes with a suitable substrate and allowing the prebiotics to be produced in situ.To our knowledge, this is the first paper describing targeted production of prebiotics in an animal model.

View Article: PubMed Central - PubMed

Affiliation: National Veterinary Institute, Technical University of Denmark, Frederiksberg, Denmark. milst@vet.dtu.dk.

ABSTRACT
Prebiotics may be efficient for prevention of intestinal infections in humans and animals by increasing the levels of beneficial bacteria and thereby improving gut health. Using purified prebiotics may however not be cost-effective in the livestock production industry. Instead, prebiotic fibres may be released directly in the gastro-intestinal tract by feeding enzymes with a suitable substrate and allowing the prebiotics to be produced in situ. Using low doses, 0.03 % enzyme-to-substrate ratio, of the enzymes pectin lyase and polygalacturonase in combination with potato pulp, a low-value industrial by-product, we show that high molecular weight galacto-rhamnogalacturonan can be solubilized in the stomach of weaning piglets. The release of this fiber is in the order of 22-38 % of the theoretical amount, achieved within 20 min. The catalysis takes place mainly in the stomach of the animal and is then followed by distribution through the small intestines. To our knowledge, this is the first paper describing targeted production of prebiotics in an animal model.

No MeSH data available.


Related in: MedlinePlus