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Urinary proteomic profiling in severe obesity and obstructive sleep apnoea with CPAP treatment.

Seetho IW, Ramírez-Torres A, Albalat A, Mullen W, Mischak H, Parker RJ, Craig S, Duffy N, Hardy KJ, Burniston JG, Wilding JP - Sleep Sci (2015)

Bottom Line: Age and BMI were not significantly different between groups.Metabolic syndrome was present in 14(52%) of those with OSA compared with 6(24%) of controls (p=0.039).Although correction for multiple testing did not reach significance, sequences were determined for 8 peptides demonstrating origins from collagens, fibrinogen beta chain and T-cadherin that may be associated with underlying cardiovascular disease mechanisms in OSA.

View Article: PubMed Central - PubMed

Affiliation: Department of Obesity & Endocrinology, University of Liverpool, UK.

ABSTRACT

Introduction: Obstructive sleep apnoea (OSA) is common in obesity and is associated with cardiovascular and metabolic complications. Continuous positive airway pressure (CPAP) in OSA may lead to physiological changes reflected in the urinary proteome. The aim of this study was to characterise the urinary proteome in severely obese adult subjects with OSA who were receiving CPAP compared with severely obese subjects without OSA.

Methods: Severely obese subjects with and without OSA were recruited. Subjects with OSA were receiving CPAP. Body composition and blood pressure measurements were recorded. Urinary samples were analysed by Capillary Electrophoresis-Mass Spectrometry (CE-MS).

Results: Twenty-seven subjects with OSA-on-CPAP (age 49±7years, BMI 43±7 kg/m(2)) and 25 controls without OSA (age 52±9years, BMI 39±4 kg/m(2)) were studied. Age and BMI were not significantly different between groups. Mean CPAP use for OSA patients was 14.5±1.0 months. Metabolic syndrome was present in 14(52%) of those with OSA compared with 6(24%) of controls (p=0.039). A urinary proteome comprising 15 peptides was identified showing differential expression between the groups (p<0.01). Although correction for multiple testing did not reach significance, sequences were determined for 8 peptides demonstrating origins from collagens, fibrinogen beta chain and T-cadherin that may be associated with underlying cardiovascular disease mechanisms in OSA.

Conclusions: The urinary proteome is compared in OSA with CPAP and without OSA in severe obesity. The effects of CPAP on OSA may lead to changes in the urinary peptides but further research work is needed to investigate the potential role for urinary proteomics in characterising urinary peptide profiles in OSA.

No MeSH data available.


Related in: MedlinePlus

3-dimensional profile of 15 peptides that showed significant differences between non-OSA (A) and OSA-on-CPAP (B) groups. The X axis represents CE migration time (minutes), Y-axis represents molecular mass (KDa, on a logarithmic scale), and the Z-axis represents mean signal intensity. In order to demonstrate differences between the 15 peptides, Figs. 2 and 3 are not of similar scale. (A) Non-OSA. (B) OSA-on-CPAP.
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f0015: 3-dimensional profile of 15 peptides that showed significant differences between non-OSA (A) and OSA-on-CPAP (B) groups. The X axis represents CE migration time (minutes), Y-axis represents molecular mass (KDa, on a logarithmic scale), and the Z-axis represents mean signal intensity. In order to demonstrate differences between the 15 peptides, Figs. 2 and 3 are not of similar scale. (A) Non-OSA. (B) OSA-on-CPAP.

Mentions: We detected 1041 different peptides that were consistently found in more than 70% of the samples in at least one of the groups. The compiled urinary proteomic data from OSA on CPAP and non-OSA patients is shown in (Fig. 2). We found 15 peptides with significant unadjusted Wilcoxon p-values (all p≤0.01) (Fig. 3). Although our initial data analysis showed a significant differential distribution in these peptides, it did not pass the more strict adjustment for multiple testing.


Urinary proteomic profiling in severe obesity and obstructive sleep apnoea with CPAP treatment.

Seetho IW, Ramírez-Torres A, Albalat A, Mullen W, Mischak H, Parker RJ, Craig S, Duffy N, Hardy KJ, Burniston JG, Wilding JP - Sleep Sci (2015)

3-dimensional profile of 15 peptides that showed significant differences between non-OSA (A) and OSA-on-CPAP (B) groups. The X axis represents CE migration time (minutes), Y-axis represents molecular mass (KDa, on a logarithmic scale), and the Z-axis represents mean signal intensity. In order to demonstrate differences between the 15 peptides, Figs. 2 and 3 are not of similar scale. (A) Non-OSA. (B) OSA-on-CPAP.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4608901&req=5

f0015: 3-dimensional profile of 15 peptides that showed significant differences between non-OSA (A) and OSA-on-CPAP (B) groups. The X axis represents CE migration time (minutes), Y-axis represents molecular mass (KDa, on a logarithmic scale), and the Z-axis represents mean signal intensity. In order to demonstrate differences between the 15 peptides, Figs. 2 and 3 are not of similar scale. (A) Non-OSA. (B) OSA-on-CPAP.
Mentions: We detected 1041 different peptides that were consistently found in more than 70% of the samples in at least one of the groups. The compiled urinary proteomic data from OSA on CPAP and non-OSA patients is shown in (Fig. 2). We found 15 peptides with significant unadjusted Wilcoxon p-values (all p≤0.01) (Fig. 3). Although our initial data analysis showed a significant differential distribution in these peptides, it did not pass the more strict adjustment for multiple testing.

Bottom Line: Age and BMI were not significantly different between groups.Metabolic syndrome was present in 14(52%) of those with OSA compared with 6(24%) of controls (p=0.039).Although correction for multiple testing did not reach significance, sequences were determined for 8 peptides demonstrating origins from collagens, fibrinogen beta chain and T-cadherin that may be associated with underlying cardiovascular disease mechanisms in OSA.

View Article: PubMed Central - PubMed

Affiliation: Department of Obesity & Endocrinology, University of Liverpool, UK.

ABSTRACT

Introduction: Obstructive sleep apnoea (OSA) is common in obesity and is associated with cardiovascular and metabolic complications. Continuous positive airway pressure (CPAP) in OSA may lead to physiological changes reflected in the urinary proteome. The aim of this study was to characterise the urinary proteome in severely obese adult subjects with OSA who were receiving CPAP compared with severely obese subjects without OSA.

Methods: Severely obese subjects with and without OSA were recruited. Subjects with OSA were receiving CPAP. Body composition and blood pressure measurements were recorded. Urinary samples were analysed by Capillary Electrophoresis-Mass Spectrometry (CE-MS).

Results: Twenty-seven subjects with OSA-on-CPAP (age 49±7years, BMI 43±7 kg/m(2)) and 25 controls without OSA (age 52±9years, BMI 39±4 kg/m(2)) were studied. Age and BMI were not significantly different between groups. Mean CPAP use for OSA patients was 14.5±1.0 months. Metabolic syndrome was present in 14(52%) of those with OSA compared with 6(24%) of controls (p=0.039). A urinary proteome comprising 15 peptides was identified showing differential expression between the groups (p<0.01). Although correction for multiple testing did not reach significance, sequences were determined for 8 peptides demonstrating origins from collagens, fibrinogen beta chain and T-cadherin that may be associated with underlying cardiovascular disease mechanisms in OSA.

Conclusions: The urinary proteome is compared in OSA with CPAP and without OSA in severe obesity. The effects of CPAP on OSA may lead to changes in the urinary peptides but further research work is needed to investigate the potential role for urinary proteomics in characterising urinary peptide profiles in OSA.

No MeSH data available.


Related in: MedlinePlus