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Urinary proteomic profiling in severe obesity and obstructive sleep apnoea with CPAP treatment.

Seetho IW, Ramírez-Torres A, Albalat A, Mullen W, Mischak H, Parker RJ, Craig S, Duffy N, Hardy KJ, Burniston JG, Wilding JP - Sleep Sci (2015)

Bottom Line: Age and BMI were not significantly different between groups.Metabolic syndrome was present in 14(52%) of those with OSA compared with 6(24%) of controls (p=0.039).Although correction for multiple testing did not reach significance, sequences were determined for 8 peptides demonstrating origins from collagens, fibrinogen beta chain and T-cadherin that may be associated with underlying cardiovascular disease mechanisms in OSA.

View Article: PubMed Central - PubMed

Affiliation: Department of Obesity & Endocrinology, University of Liverpool, UK.

ABSTRACT

Introduction: Obstructive sleep apnoea (OSA) is common in obesity and is associated with cardiovascular and metabolic complications. Continuous positive airway pressure (CPAP) in OSA may lead to physiological changes reflected in the urinary proteome. The aim of this study was to characterise the urinary proteome in severely obese adult subjects with OSA who were receiving CPAP compared with severely obese subjects without OSA.

Methods: Severely obese subjects with and without OSA were recruited. Subjects with OSA were receiving CPAP. Body composition and blood pressure measurements were recorded. Urinary samples were analysed by Capillary Electrophoresis-Mass Spectrometry (CE-MS).

Results: Twenty-seven subjects with OSA-on-CPAP (age 49±7years, BMI 43±7 kg/m(2)) and 25 controls without OSA (age 52±9years, BMI 39±4 kg/m(2)) were studied. Age and BMI were not significantly different between groups. Mean CPAP use for OSA patients was 14.5±1.0 months. Metabolic syndrome was present in 14(52%) of those with OSA compared with 6(24%) of controls (p=0.039). A urinary proteome comprising 15 peptides was identified showing differential expression between the groups (p<0.01). Although correction for multiple testing did not reach significance, sequences were determined for 8 peptides demonstrating origins from collagens, fibrinogen beta chain and T-cadherin that may be associated with underlying cardiovascular disease mechanisms in OSA.

Conclusions: The urinary proteome is compared in OSA with CPAP and without OSA in severe obesity. The effects of CPAP on OSA may lead to changes in the urinary peptides but further research work is needed to investigate the potential role for urinary proteomics in characterising urinary peptide profiles in OSA.

No MeSH data available.


Related in: MedlinePlus

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Mentions: Fifty-three subjects who had been assessed at baseline were recruited. One urinary sample could not be analysed by CE–MS and therefore was excluded from the analysis. Therefore, 52 patients were entered into the study; 27 with OSA who were on CPAP and 25 without OSA (non-OSA) (Fig. 1). All subjects were of white European ethnicity. Patients attended their follow-up after a median of 15 (IQR 13,16) months. For patients with OSA, the mean CPAP use was 14.5 months (SD 1.0) and mean CPAP use per night was 4.6 h (SD 0.6). Mean CPAP pressure was 10 cmH20 (SD 1.0).


Urinary proteomic profiling in severe obesity and obstructive sleep apnoea with CPAP treatment.

Seetho IW, Ramírez-Torres A, Albalat A, Mullen W, Mischak H, Parker RJ, Craig S, Duffy N, Hardy KJ, Burniston JG, Wilding JP - Sleep Sci (2015)

Study profile.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4608901&req=5

f0005: Study profile.
Mentions: Fifty-three subjects who had been assessed at baseline were recruited. One urinary sample could not be analysed by CE–MS and therefore was excluded from the analysis. Therefore, 52 patients were entered into the study; 27 with OSA who were on CPAP and 25 without OSA (non-OSA) (Fig. 1). All subjects were of white European ethnicity. Patients attended their follow-up after a median of 15 (IQR 13,16) months. For patients with OSA, the mean CPAP use was 14.5 months (SD 1.0) and mean CPAP use per night was 4.6 h (SD 0.6). Mean CPAP pressure was 10 cmH20 (SD 1.0).

Bottom Line: Age and BMI were not significantly different between groups.Metabolic syndrome was present in 14(52%) of those with OSA compared with 6(24%) of controls (p=0.039).Although correction for multiple testing did not reach significance, sequences were determined for 8 peptides demonstrating origins from collagens, fibrinogen beta chain and T-cadherin that may be associated with underlying cardiovascular disease mechanisms in OSA.

View Article: PubMed Central - PubMed

Affiliation: Department of Obesity & Endocrinology, University of Liverpool, UK.

ABSTRACT

Introduction: Obstructive sleep apnoea (OSA) is common in obesity and is associated with cardiovascular and metabolic complications. Continuous positive airway pressure (CPAP) in OSA may lead to physiological changes reflected in the urinary proteome. The aim of this study was to characterise the urinary proteome in severely obese adult subjects with OSA who were receiving CPAP compared with severely obese subjects without OSA.

Methods: Severely obese subjects with and without OSA were recruited. Subjects with OSA were receiving CPAP. Body composition and blood pressure measurements were recorded. Urinary samples were analysed by Capillary Electrophoresis-Mass Spectrometry (CE-MS).

Results: Twenty-seven subjects with OSA-on-CPAP (age 49±7years, BMI 43±7 kg/m(2)) and 25 controls without OSA (age 52±9years, BMI 39±4 kg/m(2)) were studied. Age and BMI were not significantly different between groups. Mean CPAP use for OSA patients was 14.5±1.0 months. Metabolic syndrome was present in 14(52%) of those with OSA compared with 6(24%) of controls (p=0.039). A urinary proteome comprising 15 peptides was identified showing differential expression between the groups (p<0.01). Although correction for multiple testing did not reach significance, sequences were determined for 8 peptides demonstrating origins from collagens, fibrinogen beta chain and T-cadherin that may be associated with underlying cardiovascular disease mechanisms in OSA.

Conclusions: The urinary proteome is compared in OSA with CPAP and without OSA in severe obesity. The effects of CPAP on OSA may lead to changes in the urinary peptides but further research work is needed to investigate the potential role for urinary proteomics in characterising urinary peptide profiles in OSA.

No MeSH data available.


Related in: MedlinePlus