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The effects of long-term dopaminergic treatment on locomotor behavior in rats.

Oliveira de Almeida WA, Maculano Esteves A, Leite de Almeida-Júnior C, Lee KS, Kannebley Frank M, Oliveira Mariano M, Frussa-Filho R, Tufik S, Tulio de Mello M - Sleep Sci (2014)

Bottom Line: The animals that received the PPX demonstrated a significant reduction in locomotor activity from day 1 to day 57 and a significant increase in immobility time from day 1 to day 64 relative to baseline values, but these values had returned to baseline levels at 71 days.No changes in the expression of the D2 receptor were demonstrated after treatment with a dopaminergic agonist.In addition, it was not possible to demonstrate a relationship between locomotor activity and the expression of D2 receptors under these conditions.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Psicobiologia, Universidade Federal de São Paulo, Brazil.

ABSTRACT
Long-term treatments with dopaminergic agents are associated with adverse effects, including augmentation. Augmentation consists of an exacerbation of restless legs syndrome (a sleep-related movement disorder) symptoms during treatment compared to those experienced during the period before therapy was initiated. The objective of this study was to examine locomotor activity in rats after long-term dopaminergic treatment and its relationship with expression of the D2 receptor, in addition to demonstrating possible evidence of augmentation. The rats were divided into control (CTRL) and drug (Pramipexole-PPX) groups that received daily saline vehicle and PPX treatments, respectively, for 71 days. The locomotor behavior of the animals was evaluated weekly in the Open Field test for 71 days. The expression of the dopamine D2 receptor was evaluated by Western Blot analysis. The animals that received the PPX demonstrated a significant reduction in locomotor activity from day 1 to day 57 and a significant increase in immobility time from day 1 to day 64 relative to baseline values, but these values had returned to baseline levels at 71 days. No changes in the expression of the D2 receptor were demonstrated after treatment with a dopaminergic agonist. This study suggests changes in locomotor activity in rats after long-term PPX treatment that include an immediate reduction of locomotion and an increase in immobilization, and after 64 days, these values returned to baseline levels without evidence of augmentation. In addition, it was not possible to demonstrate a relationship between locomotor activity and the expression of D2 receptors under these conditions.

No MeSH data available.


Related in: MedlinePlus

(A) Assessment of rearing of Wistar rats in the control (saline) and drug (pramipexole) groups in the Open Field. * indicates a significant decrease in rearing in the drug group compared to the baseline level from days 1 to 43 of treatment (ANOVA, p<0.05); @ indicates a significant decrease in rearing in the control group relative to baseline from days 15 to 29 of the treatment (ANOVA, p<0.05); # indicates a same day difference between the groups on day 1 of treatment (ANOVA, p<0.05). B assessment of grooming (time, seconds) in the Open Field in the control (saline) and drug (pramipexole) groups. * indicates a significant decrease in grooming in the drug group compared to baseline from days 1 to 71 of treatment (ANOVA, p<0.05); @ indicates a significant decrease in grooming in the control group relative to baseline from days 1 to 8 and days 22 to 71 of treatment (ANOVA, p<0.05); # indicates a same day difference between the groups on day 15 of treatment (ANOVA, p<0.05).
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f0010: (A) Assessment of rearing of Wistar rats in the control (saline) and drug (pramipexole) groups in the Open Field. * indicates a significant decrease in rearing in the drug group compared to the baseline level from days 1 to 43 of treatment (ANOVA, p<0.05); @ indicates a significant decrease in rearing in the control group relative to baseline from days 15 to 29 of the treatment (ANOVA, p<0.05); # indicates a same day difference between the groups on day 1 of treatment (ANOVA, p<0.05). B assessment of grooming (time, seconds) in the Open Field in the control (saline) and drug (pramipexole) groups. * indicates a significant decrease in grooming in the drug group compared to baseline from days 1 to 71 of treatment (ANOVA, p<0.05); @ indicates a significant decrease in grooming in the control group relative to baseline from days 1 to 8 and days 22 to 71 of treatment (ANOVA, p<0.05); # indicates a same day difference between the groups on day 15 of treatment (ANOVA, p<0.05).

Mentions: ANOVA for repeated measures revealed a significant effect for the factor time (F(11.165)= 3.8419, p<0.001). Fig. 2A shows that the drug group demonstrated a significant decrease in rearing relative to baseline from days 1 to 43 of the treatment. This figure also shows that the control group exhibited a significant decrease in rearing in relative to baseline from days 15 to 29 of the saline solution treatment. The control group exhibited a significant increase compared to the drug group on day 1 of the treatment.


The effects of long-term dopaminergic treatment on locomotor behavior in rats.

Oliveira de Almeida WA, Maculano Esteves A, Leite de Almeida-Júnior C, Lee KS, Kannebley Frank M, Oliveira Mariano M, Frussa-Filho R, Tufik S, Tulio de Mello M - Sleep Sci (2014)

(A) Assessment of rearing of Wistar rats in the control (saline) and drug (pramipexole) groups in the Open Field. * indicates a significant decrease in rearing in the drug group compared to the baseline level from days 1 to 43 of treatment (ANOVA, p<0.05); @ indicates a significant decrease in rearing in the control group relative to baseline from days 15 to 29 of the treatment (ANOVA, p<0.05); # indicates a same day difference between the groups on day 1 of treatment (ANOVA, p<0.05). B assessment of grooming (time, seconds) in the Open Field in the control (saline) and drug (pramipexole) groups. * indicates a significant decrease in grooming in the drug group compared to baseline from days 1 to 71 of treatment (ANOVA, p<0.05); @ indicates a significant decrease in grooming in the control group relative to baseline from days 1 to 8 and days 22 to 71 of treatment (ANOVA, p<0.05); # indicates a same day difference between the groups on day 15 of treatment (ANOVA, p<0.05).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4608898&req=5

f0010: (A) Assessment of rearing of Wistar rats in the control (saline) and drug (pramipexole) groups in the Open Field. * indicates a significant decrease in rearing in the drug group compared to the baseline level from days 1 to 43 of treatment (ANOVA, p<0.05); @ indicates a significant decrease in rearing in the control group relative to baseline from days 15 to 29 of the treatment (ANOVA, p<0.05); # indicates a same day difference between the groups on day 1 of treatment (ANOVA, p<0.05). B assessment of grooming (time, seconds) in the Open Field in the control (saline) and drug (pramipexole) groups. * indicates a significant decrease in grooming in the drug group compared to baseline from days 1 to 71 of treatment (ANOVA, p<0.05); @ indicates a significant decrease in grooming in the control group relative to baseline from days 1 to 8 and days 22 to 71 of treatment (ANOVA, p<0.05); # indicates a same day difference between the groups on day 15 of treatment (ANOVA, p<0.05).
Mentions: ANOVA for repeated measures revealed a significant effect for the factor time (F(11.165)= 3.8419, p<0.001). Fig. 2A shows that the drug group demonstrated a significant decrease in rearing relative to baseline from days 1 to 43 of the treatment. This figure also shows that the control group exhibited a significant decrease in rearing in relative to baseline from days 15 to 29 of the saline solution treatment. The control group exhibited a significant increase compared to the drug group on day 1 of the treatment.

Bottom Line: The animals that received the PPX demonstrated a significant reduction in locomotor activity from day 1 to day 57 and a significant increase in immobility time from day 1 to day 64 relative to baseline values, but these values had returned to baseline levels at 71 days.No changes in the expression of the D2 receptor were demonstrated after treatment with a dopaminergic agonist.In addition, it was not possible to demonstrate a relationship between locomotor activity and the expression of D2 receptors under these conditions.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Psicobiologia, Universidade Federal de São Paulo, Brazil.

ABSTRACT
Long-term treatments with dopaminergic agents are associated with adverse effects, including augmentation. Augmentation consists of an exacerbation of restless legs syndrome (a sleep-related movement disorder) symptoms during treatment compared to those experienced during the period before therapy was initiated. The objective of this study was to examine locomotor activity in rats after long-term dopaminergic treatment and its relationship with expression of the D2 receptor, in addition to demonstrating possible evidence of augmentation. The rats were divided into control (CTRL) and drug (Pramipexole-PPX) groups that received daily saline vehicle and PPX treatments, respectively, for 71 days. The locomotor behavior of the animals was evaluated weekly in the Open Field test for 71 days. The expression of the dopamine D2 receptor was evaluated by Western Blot analysis. The animals that received the PPX demonstrated a significant reduction in locomotor activity from day 1 to day 57 and a significant increase in immobility time from day 1 to day 64 relative to baseline values, but these values had returned to baseline levels at 71 days. No changes in the expression of the D2 receptor were demonstrated after treatment with a dopaminergic agonist. This study suggests changes in locomotor activity in rats after long-term PPX treatment that include an immediate reduction of locomotion and an increase in immobilization, and after 64 days, these values returned to baseline levels without evidence of augmentation. In addition, it was not possible to demonstrate a relationship between locomotor activity and the expression of D2 receptors under these conditions.

No MeSH data available.


Related in: MedlinePlus