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Water Spinach, Ipomoea aquatic (Convolvulaceae), Ameliorates Lead Toxicity by Inhibiting Oxidative Stress and Apoptosis.

Dewanjee S, Dua TK, Khanra R, Das S, Barma S, Joardar S, Bhattacharjee N, Zia-Ul-Haq M, Jaafar HZ - PLoS ONE (2015)

Bottom Line: The effects on the expressions of apoptotic signal proteins were estimated by western blotting.The extract may offer the protective effect via counteracting with Pb mediated oxidative stress and/or promoting the elimination of Pb by chelating.The presence of substantial quantities of flavonoids, phenolics and saponins would be responsible for the overall protective effect.

View Article: PubMed Central - PubMed

Affiliation: Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, 700032, India.

ABSTRACT

Background: Ipomoea aquatica (Convolvulaceae), an aquatic edible plant, is traditionally used against heavy metal toxicity in India. The current study intended to explore the protective role of edible (aqueous) extract of I. aquatica (AEIA) against experimentally induced Pb-intoxication.

Methods: The cytoprotective role of AEIA was measured on mouse hepatocytes by cell viability assay followed by Hoechst staining and flow cytometric assay. The effect on ROS production, lipid peroxidation, protein carbonylation, intracellular redox status were measured after incubating the hepatocytes with Pb-acetate (6.8 μM) along with AEIA (400 μg/ml). The effects on the expressions of apoptotic signal proteins were estimated by western blotting. The protective role of AEIA was measured by in vivo assay in mice. Haematological, serum biochemical, tissue redox status, Pb bioaccumulation and histological parameters were evaluated to estimate the protective role of AEIA (100 mg/kg) against Pb-acetate (5 mg/kg) intoxication.

Results: Pb-acetate treated hepatocytes showed a gradual reduction of cell viability dose-dependently with an IC50 value of 6.8 μM. Pb-acetate treated hepatocytes exhibited significantly enhanced levels (p < 0.01) of ROS production, lipid peroxidation, protein carbonylation with concomitant depletion (p < 0.01) of antioxidant enzymes and GSH. However, AEIA treatment could significantly restore the aforementioned parameters in murine hepatocytes near to normalcy. Besides, AEIA significantly reversed (p < 0.05-0.01) the alterations of transcription levels of apoptotic proteins viz. Bcl 2, Bad, Cyt C, Apaf-1, cleaved caspases [caspase 3, caspase 8 and caspase 9], Fas and Bid. In in vivo bioassay, Pb-acetate treatment caused significantly high intracellular Pb burden and oxidative pressure in the kidney, liver, heart, brain and testes in mice. In addition, the haematological and serum biochemical factors were changed significantly in Pb-acetate-treated animals. AEIA treatment restored significantly the evaluated-parameters to the near-normal position.

Conclusion: The extract may offer the protective effect via counteracting with Pb mediated oxidative stress and/or promoting the elimination of Pb by chelating. The presence of substantial quantities of flavonoids, phenolics and saponins would be responsible for the overall protective effect.

No MeSH data available.


Related in: MedlinePlus

Schematic presentation of probable protective mechanism of AEIA against Pb toxicity.The blue arrows indicate the cellular events involved in Pb pathogenesis. The green colour line denotes the site of action of AEIA.
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pone.0139831.g012: Schematic presentation of probable protective mechanism of AEIA against Pb toxicity.The blue arrows indicate the cellular events involved in Pb pathogenesis. The green colour line denotes the site of action of AEIA.

Mentions: Existing literature revealed that, bioaccumulation of Pb causes generation of excessive ROS which could cause redox imbalance and participate pivotal role in Pb-toxicity. Therefore, the agents promoting Pb clearance and/or scavenging ROS would participate in counteracting Pb-poisoning. In present investigation, excessive generation of ROS following Pb exposure caused oxidative tissue damage via apoptosis and hampered cellular redox defence system (Fig 12). Experimental data revealed that the treatment of the edible extract of the edible aerial parts of I. aquatica could cause significant reversal of Pb-acetate induced toxic manifestations in both in vivo and in vitro systems. The extract would offer the protective effect via counteracting with Pb mediated oxidative stress and/or promoting the elimination of Pb by chelating (Fig 12). Phytochemical studies revealed presence of flavonoids, phenolic compounds and ascorbic acid in the test extracts. Substantial quantities of aforementioned dietary antioxidants [9,22,24] could contribute in the ROS scavenging during Pb-intoxication. Besides, saponins and flavonoids are known possess Pb chelating property [39,40]. Therefore, dietary antioxidants would serve as primary healthcare against ROS mediated Pb poisoning and the diet comprising I. aquatica would serve as useful clinical medicine against Pd toxicity. Present study is continued to isolate scaffolds for the overall protective effect of I. aquatica.


Water Spinach, Ipomoea aquatic (Convolvulaceae), Ameliorates Lead Toxicity by Inhibiting Oxidative Stress and Apoptosis.

Dewanjee S, Dua TK, Khanra R, Das S, Barma S, Joardar S, Bhattacharjee N, Zia-Ul-Haq M, Jaafar HZ - PLoS ONE (2015)

Schematic presentation of probable protective mechanism of AEIA against Pb toxicity.The blue arrows indicate the cellular events involved in Pb pathogenesis. The green colour line denotes the site of action of AEIA.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4608788&req=5

pone.0139831.g012: Schematic presentation of probable protective mechanism of AEIA against Pb toxicity.The blue arrows indicate the cellular events involved in Pb pathogenesis. The green colour line denotes the site of action of AEIA.
Mentions: Existing literature revealed that, bioaccumulation of Pb causes generation of excessive ROS which could cause redox imbalance and participate pivotal role in Pb-toxicity. Therefore, the agents promoting Pb clearance and/or scavenging ROS would participate in counteracting Pb-poisoning. In present investigation, excessive generation of ROS following Pb exposure caused oxidative tissue damage via apoptosis and hampered cellular redox defence system (Fig 12). Experimental data revealed that the treatment of the edible extract of the edible aerial parts of I. aquatica could cause significant reversal of Pb-acetate induced toxic manifestations in both in vivo and in vitro systems. The extract would offer the protective effect via counteracting with Pb mediated oxidative stress and/or promoting the elimination of Pb by chelating (Fig 12). Phytochemical studies revealed presence of flavonoids, phenolic compounds and ascorbic acid in the test extracts. Substantial quantities of aforementioned dietary antioxidants [9,22,24] could contribute in the ROS scavenging during Pb-intoxication. Besides, saponins and flavonoids are known possess Pb chelating property [39,40]. Therefore, dietary antioxidants would serve as primary healthcare against ROS mediated Pb poisoning and the diet comprising I. aquatica would serve as useful clinical medicine against Pd toxicity. Present study is continued to isolate scaffolds for the overall protective effect of I. aquatica.

Bottom Line: The effects on the expressions of apoptotic signal proteins were estimated by western blotting.The extract may offer the protective effect via counteracting with Pb mediated oxidative stress and/or promoting the elimination of Pb by chelating.The presence of substantial quantities of flavonoids, phenolics and saponins would be responsible for the overall protective effect.

View Article: PubMed Central - PubMed

Affiliation: Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, 700032, India.

ABSTRACT

Background: Ipomoea aquatica (Convolvulaceae), an aquatic edible plant, is traditionally used against heavy metal toxicity in India. The current study intended to explore the protective role of edible (aqueous) extract of I. aquatica (AEIA) against experimentally induced Pb-intoxication.

Methods: The cytoprotective role of AEIA was measured on mouse hepatocytes by cell viability assay followed by Hoechst staining and flow cytometric assay. The effect on ROS production, lipid peroxidation, protein carbonylation, intracellular redox status were measured after incubating the hepatocytes with Pb-acetate (6.8 μM) along with AEIA (400 μg/ml). The effects on the expressions of apoptotic signal proteins were estimated by western blotting. The protective role of AEIA was measured by in vivo assay in mice. Haematological, serum biochemical, tissue redox status, Pb bioaccumulation and histological parameters were evaluated to estimate the protective role of AEIA (100 mg/kg) against Pb-acetate (5 mg/kg) intoxication.

Results: Pb-acetate treated hepatocytes showed a gradual reduction of cell viability dose-dependently with an IC50 value of 6.8 μM. Pb-acetate treated hepatocytes exhibited significantly enhanced levels (p < 0.01) of ROS production, lipid peroxidation, protein carbonylation with concomitant depletion (p < 0.01) of antioxidant enzymes and GSH. However, AEIA treatment could significantly restore the aforementioned parameters in murine hepatocytes near to normalcy. Besides, AEIA significantly reversed (p < 0.05-0.01) the alterations of transcription levels of apoptotic proteins viz. Bcl 2, Bad, Cyt C, Apaf-1, cleaved caspases [caspase 3, caspase 8 and caspase 9], Fas and Bid. In in vivo bioassay, Pb-acetate treatment caused significantly high intracellular Pb burden and oxidative pressure in the kidney, liver, heart, brain and testes in mice. In addition, the haematological and serum biochemical factors were changed significantly in Pb-acetate-treated animals. AEIA treatment restored significantly the evaluated-parameters to the near-normal position.

Conclusion: The extract may offer the protective effect via counteracting with Pb mediated oxidative stress and/or promoting the elimination of Pb by chelating. The presence of substantial quantities of flavonoids, phenolics and saponins would be responsible for the overall protective effect.

No MeSH data available.


Related in: MedlinePlus