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Water Spinach, Ipomoea aquatic (Convolvulaceae), Ameliorates Lead Toxicity by Inhibiting Oxidative Stress and Apoptosis.

Dewanjee S, Dua TK, Khanra R, Das S, Barma S, Joardar S, Bhattacharjee N, Zia-Ul-Haq M, Jaafar HZ - PLoS ONE (2015)

Bottom Line: The effects on the expressions of apoptotic signal proteins were estimated by western blotting.The extract may offer the protective effect via counteracting with Pb mediated oxidative stress and/or promoting the elimination of Pb by chelating.The presence of substantial quantities of flavonoids, phenolics and saponins would be responsible for the overall protective effect.

View Article: PubMed Central - PubMed

Affiliation: Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, 700032, India.

ABSTRACT

Background: Ipomoea aquatica (Convolvulaceae), an aquatic edible plant, is traditionally used against heavy metal toxicity in India. The current study intended to explore the protective role of edible (aqueous) extract of I. aquatica (AEIA) against experimentally induced Pb-intoxication.

Methods: The cytoprotective role of AEIA was measured on mouse hepatocytes by cell viability assay followed by Hoechst staining and flow cytometric assay. The effect on ROS production, lipid peroxidation, protein carbonylation, intracellular redox status were measured after incubating the hepatocytes with Pb-acetate (6.8 μM) along with AEIA (400 μg/ml). The effects on the expressions of apoptotic signal proteins were estimated by western blotting. The protective role of AEIA was measured by in vivo assay in mice. Haematological, serum biochemical, tissue redox status, Pb bioaccumulation and histological parameters were evaluated to estimate the protective role of AEIA (100 mg/kg) against Pb-acetate (5 mg/kg) intoxication.

Results: Pb-acetate treated hepatocytes showed a gradual reduction of cell viability dose-dependently with an IC50 value of 6.8 μM. Pb-acetate treated hepatocytes exhibited significantly enhanced levels (p < 0.01) of ROS production, lipid peroxidation, protein carbonylation with concomitant depletion (p < 0.01) of antioxidant enzymes and GSH. However, AEIA treatment could significantly restore the aforementioned parameters in murine hepatocytes near to normalcy. Besides, AEIA significantly reversed (p < 0.05-0.01) the alterations of transcription levels of apoptotic proteins viz. Bcl 2, Bad, Cyt C, Apaf-1, cleaved caspases [caspase 3, caspase 8 and caspase 9], Fas and Bid. In in vivo bioassay, Pb-acetate treatment caused significantly high intracellular Pb burden and oxidative pressure in the kidney, liver, heart, brain and testes in mice. In addition, the haematological and serum biochemical factors were changed significantly in Pb-acetate-treated animals. AEIA treatment restored significantly the evaluated-parameters to the near-normal position.

Conclusion: The extract may offer the protective effect via counteracting with Pb mediated oxidative stress and/or promoting the elimination of Pb by chelating. The presence of substantial quantities of flavonoids, phenolics and saponins would be responsible for the overall protective effect.

No MeSH data available.


Related in: MedlinePlus

Effect on Pb-bioaccumulation (A), DNA fragmentation (B) and ATP level (C) in the absence (Pb-acetate) and existence of AEIA (Pb-acetate +AEIA) in liver, kidney, heart, brain and testes in experimental mice.Values are denoted as mean ± SE (n = 6). # Values significantly differed (p < 0.01) from normal control. *Values significantly differed (p < 0.05) from differed Pb-acetate control. ** Values significantly differed (p < 0.01) from differed Pb-acetate control.
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pone.0139831.g005: Effect on Pb-bioaccumulation (A), DNA fragmentation (B) and ATP level (C) in the absence (Pb-acetate) and existence of AEIA (Pb-acetate +AEIA) in liver, kidney, heart, brain and testes in experimental mice.Values are denoted as mean ± SE (n = 6). # Values significantly differed (p < 0.01) from normal control. *Values significantly differed (p < 0.05) from differed Pb-acetate control. ** Values significantly differed (p < 0.01) from differed Pb-acetate control.

Mentions: The accumulation of Pb in the tissues is thought to be the principle cause of Pb-intoxication. The effect of Pb-acetate treatment in the intracellular Pb-burden has been estimated in kidney, liver, heart, brain and testes of experimental mice (Fig 5). Pb-acetate treatment significantly increased (p < 0.01) the Pb-bioaccumulation in the aforementioned organs. The degree of Pb accumulation was found to in the order of kidney > liver > heart > testes > brain. However, AEIA treatment could significantly (p < 0.01) prevent intracellular Pb burden in the aforementioned tissues, as compared to Pb-acetate treated mice. Data set is available in S4 Table.


Water Spinach, Ipomoea aquatic (Convolvulaceae), Ameliorates Lead Toxicity by Inhibiting Oxidative Stress and Apoptosis.

Dewanjee S, Dua TK, Khanra R, Das S, Barma S, Joardar S, Bhattacharjee N, Zia-Ul-Haq M, Jaafar HZ - PLoS ONE (2015)

Effect on Pb-bioaccumulation (A), DNA fragmentation (B) and ATP level (C) in the absence (Pb-acetate) and existence of AEIA (Pb-acetate +AEIA) in liver, kidney, heart, brain and testes in experimental mice.Values are denoted as mean ± SE (n = 6). # Values significantly differed (p < 0.01) from normal control. *Values significantly differed (p < 0.05) from differed Pb-acetate control. ** Values significantly differed (p < 0.01) from differed Pb-acetate control.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4608788&req=5

pone.0139831.g005: Effect on Pb-bioaccumulation (A), DNA fragmentation (B) and ATP level (C) in the absence (Pb-acetate) and existence of AEIA (Pb-acetate +AEIA) in liver, kidney, heart, brain and testes in experimental mice.Values are denoted as mean ± SE (n = 6). # Values significantly differed (p < 0.01) from normal control. *Values significantly differed (p < 0.05) from differed Pb-acetate control. ** Values significantly differed (p < 0.01) from differed Pb-acetate control.
Mentions: The accumulation of Pb in the tissues is thought to be the principle cause of Pb-intoxication. The effect of Pb-acetate treatment in the intracellular Pb-burden has been estimated in kidney, liver, heart, brain and testes of experimental mice (Fig 5). Pb-acetate treatment significantly increased (p < 0.01) the Pb-bioaccumulation in the aforementioned organs. The degree of Pb accumulation was found to in the order of kidney > liver > heart > testes > brain. However, AEIA treatment could significantly (p < 0.01) prevent intracellular Pb burden in the aforementioned tissues, as compared to Pb-acetate treated mice. Data set is available in S4 Table.

Bottom Line: The effects on the expressions of apoptotic signal proteins were estimated by western blotting.The extract may offer the protective effect via counteracting with Pb mediated oxidative stress and/or promoting the elimination of Pb by chelating.The presence of substantial quantities of flavonoids, phenolics and saponins would be responsible for the overall protective effect.

View Article: PubMed Central - PubMed

Affiliation: Advanced Pharmacognosy Research Laboratory, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, 700032, India.

ABSTRACT

Background: Ipomoea aquatica (Convolvulaceae), an aquatic edible plant, is traditionally used against heavy metal toxicity in India. The current study intended to explore the protective role of edible (aqueous) extract of I. aquatica (AEIA) against experimentally induced Pb-intoxication.

Methods: The cytoprotective role of AEIA was measured on mouse hepatocytes by cell viability assay followed by Hoechst staining and flow cytometric assay. The effect on ROS production, lipid peroxidation, protein carbonylation, intracellular redox status were measured after incubating the hepatocytes with Pb-acetate (6.8 μM) along with AEIA (400 μg/ml). The effects on the expressions of apoptotic signal proteins were estimated by western blotting. The protective role of AEIA was measured by in vivo assay in mice. Haematological, serum biochemical, tissue redox status, Pb bioaccumulation and histological parameters were evaluated to estimate the protective role of AEIA (100 mg/kg) against Pb-acetate (5 mg/kg) intoxication.

Results: Pb-acetate treated hepatocytes showed a gradual reduction of cell viability dose-dependently with an IC50 value of 6.8 μM. Pb-acetate treated hepatocytes exhibited significantly enhanced levels (p < 0.01) of ROS production, lipid peroxidation, protein carbonylation with concomitant depletion (p < 0.01) of antioxidant enzymes and GSH. However, AEIA treatment could significantly restore the aforementioned parameters in murine hepatocytes near to normalcy. Besides, AEIA significantly reversed (p < 0.05-0.01) the alterations of transcription levels of apoptotic proteins viz. Bcl 2, Bad, Cyt C, Apaf-1, cleaved caspases [caspase 3, caspase 8 and caspase 9], Fas and Bid. In in vivo bioassay, Pb-acetate treatment caused significantly high intracellular Pb burden and oxidative pressure in the kidney, liver, heart, brain and testes in mice. In addition, the haematological and serum biochemical factors were changed significantly in Pb-acetate-treated animals. AEIA treatment restored significantly the evaluated-parameters to the near-normal position.

Conclusion: The extract may offer the protective effect via counteracting with Pb mediated oxidative stress and/or promoting the elimination of Pb by chelating. The presence of substantial quantities of flavonoids, phenolics and saponins would be responsible for the overall protective effect.

No MeSH data available.


Related in: MedlinePlus