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Waterborne Risperidone Decreases Stress Response in Zebrafish.

Idalencio R, Kalichak F, Rosa JG, de Oliveira TA, Koakoski G, Gusso D, Abreu MS, Giacomini AC, Barcellos HH, Piato AL, Barcellos LJ - PLoS ONE (2015)

Bottom Line: The presence of drugs and their metabolites in surface waters and municipal effluents has been reported in several studies, but its impacts on aquatic organisms are not yet well understood.This study investigated the effects of acute exposure to the antipsychotic risperidone on the stress and behavioral responses in zebrafish.It became clear that intermediate concentration of risperidone inhibited the hypothalamic-pituitary-interrenal axis and displayed anxiolytic-like effects in zebrafish.

View Article: PubMed Central - PubMed

Affiliation: Programa de Pós-Graduação em Bioexperimentação, Universidade de Passo Fundo (UPF), Passo Fundo, RS, Brasil; Laboratório de Fisiologia de Peixes, Universidade de Passo Fundo (UPF), Passo Fundo, RS, Brasil.

ABSTRACT
The presence of drugs and their metabolites in surface waters and municipal effluents has been reported in several studies, but its impacts on aquatic organisms are not yet well understood. This study investigated the effects of acute exposure to the antipsychotic risperidone on the stress and behavioral responses in zebrafish. It became clear that intermediate concentration of risperidone inhibited the hypothalamic-pituitary-interrenal axis and displayed anxiolytic-like effects in zebrafish. The data presented here suggest that the presence of this antipsychotic in aquatic environments can alter neuroendocrine and behavior profiles in zebrafish.

No MeSH data available.


Related in: MedlinePlus

Whole-body cortisol concentrations in zebrafish exposed to risperidone followed by an acute stress test and respective controls.The values are expressed as the mean ± standard error of mean of 5–6 fish. Different small letters indicate significant group differences in each sampling time. The insert shows graphical demonstration of U-shaped dose response curve at the time of cortisol peak. S- and S+ refer to non-stressed and stressed fish, respectively.
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pone.0140800.g001: Whole-body cortisol concentrations in zebrafish exposed to risperidone followed by an acute stress test and respective controls.The values are expressed as the mean ± standard error of mean of 5–6 fish. Different small letters indicate significant group differences in each sampling time. The insert shows graphical demonstration of U-shaped dose response curve at the time of cortisol peak. S- and S+ refer to non-stressed and stressed fish, respectively.

Mentions: We performed a behavioral test (novel tank test [26, 27]) using risperidone at the concentration of 170 μg/L, which impaired cortisol response in experiment 1 (see results section, Fig 1). For this purpose, 24 zebrafish not exposed in the experiment 1, were distributed in four groups: (1) control (no exposure to risperidone, no stress), (2) risperidone exposed, (3) stress and (4) risperidone exposed + stress. After 15 min of exposure, fish were individually transferred to the novel tank (4 x 20 x 20 cm) and video recorded for 5 minutes, 15 min after stressor application. The videos were then analyzed using AnyMaze® video tracking system (Stoelting, CO, USA) and the following parameters were analyzed: total distance (m), mean speed (m/s), absolute turn angle, number of crossings between different compartments of the tank (upper, middle and bottom) and the time spent in upper, middle and bottom compartments.


Waterborne Risperidone Decreases Stress Response in Zebrafish.

Idalencio R, Kalichak F, Rosa JG, de Oliveira TA, Koakoski G, Gusso D, Abreu MS, Giacomini AC, Barcellos HH, Piato AL, Barcellos LJ - PLoS ONE (2015)

Whole-body cortisol concentrations in zebrafish exposed to risperidone followed by an acute stress test and respective controls.The values are expressed as the mean ± standard error of mean of 5–6 fish. Different small letters indicate significant group differences in each sampling time. The insert shows graphical demonstration of U-shaped dose response curve at the time of cortisol peak. S- and S+ refer to non-stressed and stressed fish, respectively.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4608780&req=5

pone.0140800.g001: Whole-body cortisol concentrations in zebrafish exposed to risperidone followed by an acute stress test and respective controls.The values are expressed as the mean ± standard error of mean of 5–6 fish. Different small letters indicate significant group differences in each sampling time. The insert shows graphical demonstration of U-shaped dose response curve at the time of cortisol peak. S- and S+ refer to non-stressed and stressed fish, respectively.
Mentions: We performed a behavioral test (novel tank test [26, 27]) using risperidone at the concentration of 170 μg/L, which impaired cortisol response in experiment 1 (see results section, Fig 1). For this purpose, 24 zebrafish not exposed in the experiment 1, were distributed in four groups: (1) control (no exposure to risperidone, no stress), (2) risperidone exposed, (3) stress and (4) risperidone exposed + stress. After 15 min of exposure, fish were individually transferred to the novel tank (4 x 20 x 20 cm) and video recorded for 5 minutes, 15 min after stressor application. The videos were then analyzed using AnyMaze® video tracking system (Stoelting, CO, USA) and the following parameters were analyzed: total distance (m), mean speed (m/s), absolute turn angle, number of crossings between different compartments of the tank (upper, middle and bottom) and the time spent in upper, middle and bottom compartments.

Bottom Line: The presence of drugs and their metabolites in surface waters and municipal effluents has been reported in several studies, but its impacts on aquatic organisms are not yet well understood.This study investigated the effects of acute exposure to the antipsychotic risperidone on the stress and behavioral responses in zebrafish.It became clear that intermediate concentration of risperidone inhibited the hypothalamic-pituitary-interrenal axis and displayed anxiolytic-like effects in zebrafish.

View Article: PubMed Central - PubMed

Affiliation: Programa de Pós-Graduação em Bioexperimentação, Universidade de Passo Fundo (UPF), Passo Fundo, RS, Brasil; Laboratório de Fisiologia de Peixes, Universidade de Passo Fundo (UPF), Passo Fundo, RS, Brasil.

ABSTRACT
The presence of drugs and their metabolites in surface waters and municipal effluents has been reported in several studies, but its impacts on aquatic organisms are not yet well understood. This study investigated the effects of acute exposure to the antipsychotic risperidone on the stress and behavioral responses in zebrafish. It became clear that intermediate concentration of risperidone inhibited the hypothalamic-pituitary-interrenal axis and displayed anxiolytic-like effects in zebrafish. The data presented here suggest that the presence of this antipsychotic in aquatic environments can alter neuroendocrine and behavior profiles in zebrafish.

No MeSH data available.


Related in: MedlinePlus