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The Roles of Arabidopsis CDF2 in Transcriptional and Posttranscriptional Regulation of Primary MicroRNAs.

Sun Z, Guo T, Liu Y, Liu Q, Fang Y - PLoS Genet. (2015)

Bottom Line: CDF2 binds directly to the promoters of some miRNAs and works as a transcription activator or repressor for these miRNA genes.CDF2 binds preferentially to the pri-miRNAs regulated by itself and affects DCL1-mediated processing of these pri-miRNAs.We conclude that CDF2 regulates a group of pri-miRNAs at both the transcriptional and posttranscriptional levels to maintain proper levels of their mature miRNAs to control plant development.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.

ABSTRACT
The precise regulation of microRNA (miRNA) transcription and processing is important for eukaryotic development. Plant miRNAs are first transcribed as stem-loop primary miRNAs (pri-miRNAs) by RNA polymerase II,then cleaved in the nucleus into mature miRNAs by Dicer-like 1 (DCL1). We identified a cycling DOF transcription factor, CDF2, which interacts with DCL1 and regulates the accumulation of a population of miRNAs. CDF2 binds directly to the promoters of some miRNAs and works as a transcription activator or repressor for these miRNA genes. CDF2 binds preferentially to the pri-miRNAs regulated by itself and affects DCL1-mediated processing of these pri-miRNAs. Genetically, CDF2 works in the same pathway as miR156 or miR172 to control flowering. We conclude that CDF2 regulates a group of pri-miRNAs at both the transcriptional and posttranscriptional levels to maintain proper levels of their mature miRNAs to control plant development.

No MeSH data available.


CDF2 suppresses the posttranscriptional processing of pri-miRNAs.(A) Northern blots show that overexpression of CDF2 reduce the accumulation of miRNAs indicated. (B) Northern blots show that the levels of miR156 and miR172 in p35S::miR156/Col and p35S::miR156/cdf2 or p35S::miR172/Col and p35S::miR172/cdf2 plants.
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pgen.1005598.g005: CDF2 suppresses the posttranscriptional processing of pri-miRNAs.(A) Northern blots show that overexpression of CDF2 reduce the accumulation of miRNAs indicated. (B) Northern blots show that the levels of miR156 and miR172 in p35S::miR156/Col and p35S::miR156/cdf2 or p35S::miR172/Col and p35S::miR172/cdf2 plants.

Mentions: As CDF2 interacts with DCL1, and affects the binding of DCL1 to pri-miRNAs (Figs 4A and S7A–S7D), we then evaluated the role of CDF2 in DCL1-mediated miRNA processing in vivo. To this end, we compared the accumulation levels of five miRNAs, including miR156, miR167, miR319, and miR172 which are regulated by CDF2, and miR164 which is not regulated by CDF2, in Col, cdf2, p35S::CDF2-YFP/Col (S10A Fig), and dcl1-9 lines. We found that the accumulation levels of miR156, miR167, miR319, and miR172 in p35S::CDF2-YFP/Col were significantly lower than that in cdf2 mutant and Col lines (Fig 5A). In contrast, miR164 accumulation level in the p35S::CDF2-YFP/Col line is similar to that in Col, possibly due to that CDF2 does not bind to miRNA164 in vivo which is not regulated at the transcriptional level by CDF2 (Fig 4B). We also observed that the levels of CDF2 and miRNAs in plants expressing CDF2-YFP under the control of its endogenous promoter (pCDF2::CDF2-YFP/Col) were very similar to those in Col (S10A and S10B Fig).


The Roles of Arabidopsis CDF2 in Transcriptional and Posttranscriptional Regulation of Primary MicroRNAs.

Sun Z, Guo T, Liu Y, Liu Q, Fang Y - PLoS Genet. (2015)

CDF2 suppresses the posttranscriptional processing of pri-miRNAs.(A) Northern blots show that overexpression of CDF2 reduce the accumulation of miRNAs indicated. (B) Northern blots show that the levels of miR156 and miR172 in p35S::miR156/Col and p35S::miR156/cdf2 or p35S::miR172/Col and p35S::miR172/cdf2 plants.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4608766&req=5

pgen.1005598.g005: CDF2 suppresses the posttranscriptional processing of pri-miRNAs.(A) Northern blots show that overexpression of CDF2 reduce the accumulation of miRNAs indicated. (B) Northern blots show that the levels of miR156 and miR172 in p35S::miR156/Col and p35S::miR156/cdf2 or p35S::miR172/Col and p35S::miR172/cdf2 plants.
Mentions: As CDF2 interacts with DCL1, and affects the binding of DCL1 to pri-miRNAs (Figs 4A and S7A–S7D), we then evaluated the role of CDF2 in DCL1-mediated miRNA processing in vivo. To this end, we compared the accumulation levels of five miRNAs, including miR156, miR167, miR319, and miR172 which are regulated by CDF2, and miR164 which is not regulated by CDF2, in Col, cdf2, p35S::CDF2-YFP/Col (S10A Fig), and dcl1-9 lines. We found that the accumulation levels of miR156, miR167, miR319, and miR172 in p35S::CDF2-YFP/Col were significantly lower than that in cdf2 mutant and Col lines (Fig 5A). In contrast, miR164 accumulation level in the p35S::CDF2-YFP/Col line is similar to that in Col, possibly due to that CDF2 does not bind to miRNA164 in vivo which is not regulated at the transcriptional level by CDF2 (Fig 4B). We also observed that the levels of CDF2 and miRNAs in plants expressing CDF2-YFP under the control of its endogenous promoter (pCDF2::CDF2-YFP/Col) were very similar to those in Col (S10A and S10B Fig).

Bottom Line: CDF2 binds directly to the promoters of some miRNAs and works as a transcription activator or repressor for these miRNA genes.CDF2 binds preferentially to the pri-miRNAs regulated by itself and affects DCL1-mediated processing of these pri-miRNAs.We conclude that CDF2 regulates a group of pri-miRNAs at both the transcriptional and posttranscriptional levels to maintain proper levels of their mature miRNAs to control plant development.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, China.

ABSTRACT
The precise regulation of microRNA (miRNA) transcription and processing is important for eukaryotic development. Plant miRNAs are first transcribed as stem-loop primary miRNAs (pri-miRNAs) by RNA polymerase II,then cleaved in the nucleus into mature miRNAs by Dicer-like 1 (DCL1). We identified a cycling DOF transcription factor, CDF2, which interacts with DCL1 and regulates the accumulation of a population of miRNAs. CDF2 binds directly to the promoters of some miRNAs and works as a transcription activator or repressor for these miRNA genes. CDF2 binds preferentially to the pri-miRNAs regulated by itself and affects DCL1-mediated processing of these pri-miRNAs. Genetically, CDF2 works in the same pathway as miR156 or miR172 to control flowering. We conclude that CDF2 regulates a group of pri-miRNAs at both the transcriptional and posttranscriptional levels to maintain proper levels of their mature miRNAs to control plant development.

No MeSH data available.