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Multiparametric MRI of Epiphyseal Cartilage Necrosis (Osteochondrosis) with Histological Validation in a Goat Model.

Wang L, Nissi MJ, Tóth F, Shaver J, Johnson CP, Zhang J, Garwood M, Carlson CS, Ellermann JM - PLoS ONE (2015)

Bottom Line: All relaxation time constants differentiated normal epiphyseal cartilage from lesions of ischemic cartilage necrosis, and the histological staining results confirmed the proteoglycan (PG) loss in the areas of ischemia.In the scanned specimens, all of the measured relaxation time constants were higher in the articular than in the normal epiphyseal cartilage, consistently allowing differentiation between these two tissues.Multiparametric MRI provided a sensitive approach to discriminate between necrotic and viable epiphyseal cartilage and between articular and epiphyseal cartilage, which may be useful for diagnosing and monitoring OC lesions and, potentially, for assessing effectiveness of treatment interventions.

View Article: PubMed Central - PubMed

Affiliation: Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota, Minneapolis, MN, United States of America; Department of Orthopaedic Surgery, University of Minnesota, Minneapolis, MN, United States of America.

ABSTRACT

Purpose: To evaluate multiple MRI parameters in a surgical model of osteochondrosis (OC) in goats.

Methods: Focal ischemic lesions of two different sizes were induced in the epiphyseal cartilage of the medial femoral condyles of goats at 4 days of age by surgical transection of cartilage canal blood vessels. Goats were euthanized and specimens harvested 3, 4, 5, 6, 9 and 10 weeks post-op. Ex vivo MRI scans were conducted at 9.4 Tesla for mapping the T1, T2, T1ρ, adiabatic T1ρ and TRAFF relaxation times of articular cartilage, unaffected epiphyseal cartilage, and epiphyseal cartilage within the area of the induced lesion. After MRI scans, safranin O staining was conducted to validate areas of ischemic necrosis induced in the medial femoral condyles of six goats, and to allow comparison of MRI findings with the semi-quantitative proteoglycan assessment in corresponding safranin O-stained histological sections.

Results: All relaxation time constants differentiated normal epiphyseal cartilage from lesions of ischemic cartilage necrosis, and the histological staining results confirmed the proteoglycan (PG) loss in the areas of ischemia. In the scanned specimens, all of the measured relaxation time constants were higher in the articular than in the normal epiphyseal cartilage, consistently allowing differentiation between these two tissues.

Conclusions: Multiparametric MRI provided a sensitive approach to discriminate between necrotic and viable epiphyseal cartilage and between articular and epiphyseal cartilage, which may be useful for diagnosing and monitoring OC lesions and, potentially, for assessing effectiveness of treatment interventions.

No MeSH data available.


Related in: MedlinePlus

Safranin O-stained sections of femoral condyle.Top row: Surgically induced large lesions (3, 5, and 9 weeks post induction). Bottom row: Surgically induced small lesions (4, 6 and 10 weeks post surgical induction). Decreased staining in the chondronecrosis shows a variable degree of pallor. The optical density experiment of the safranin O-stained sections of the femoral condyle was conducted and shown in Fig 2 to estimate the PG loss in the chondronecrosis.
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pone.0140400.g001: Safranin O-stained sections of femoral condyle.Top row: Surgically induced large lesions (3, 5, and 9 weeks post induction). Bottom row: Surgically induced small lesions (4, 6 and 10 weeks post surgical induction). Decreased staining in the chondronecrosis shows a variable degree of pallor. The optical density experiment of the safranin O-stained sections of the femoral condyle was conducted and shown in Fig 2 to estimate the PG loss in the chondronecrosis.

Mentions: In 5 out of 6 goats, surgical interruption of the vascular supply to the MFC resulted in a well-circumscribed area of epiphyseal cartilage necrosis, characterized by necrotic chondrocytes within an area of matrix that exhibited a variable degree of pallor in the safranin O stained (Fig 1) and H&E-stained sections [33]. The lesion in the goat that was euthanized 6 weeks after surgery contained no histologically identifiable area of cartilage necrosis (Fig 1E). The average size of the area of cartilage necrosis in the goats in which a small (3 × 4 mm2) incision was made was 0.55 mm2 (range 0.5–0.6 mm2) whereas that in which the larger (5 × 5 mm2) incision was made was 2.8 mm2 (range 1.2–5.3 mm2) [33]. None of the lesions involved the overlying articular cartilage, which is avascular throughout life and does not depend on a blood supply from cartilage canal vessels.


Multiparametric MRI of Epiphyseal Cartilage Necrosis (Osteochondrosis) with Histological Validation in a Goat Model.

Wang L, Nissi MJ, Tóth F, Shaver J, Johnson CP, Zhang J, Garwood M, Carlson CS, Ellermann JM - PLoS ONE (2015)

Safranin O-stained sections of femoral condyle.Top row: Surgically induced large lesions (3, 5, and 9 weeks post induction). Bottom row: Surgically induced small lesions (4, 6 and 10 weeks post surgical induction). Decreased staining in the chondronecrosis shows a variable degree of pallor. The optical density experiment of the safranin O-stained sections of the femoral condyle was conducted and shown in Fig 2 to estimate the PG loss in the chondronecrosis.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4608749&req=5

pone.0140400.g001: Safranin O-stained sections of femoral condyle.Top row: Surgically induced large lesions (3, 5, and 9 weeks post induction). Bottom row: Surgically induced small lesions (4, 6 and 10 weeks post surgical induction). Decreased staining in the chondronecrosis shows a variable degree of pallor. The optical density experiment of the safranin O-stained sections of the femoral condyle was conducted and shown in Fig 2 to estimate the PG loss in the chondronecrosis.
Mentions: In 5 out of 6 goats, surgical interruption of the vascular supply to the MFC resulted in a well-circumscribed area of epiphyseal cartilage necrosis, characterized by necrotic chondrocytes within an area of matrix that exhibited a variable degree of pallor in the safranin O stained (Fig 1) and H&E-stained sections [33]. The lesion in the goat that was euthanized 6 weeks after surgery contained no histologically identifiable area of cartilage necrosis (Fig 1E). The average size of the area of cartilage necrosis in the goats in which a small (3 × 4 mm2) incision was made was 0.55 mm2 (range 0.5–0.6 mm2) whereas that in which the larger (5 × 5 mm2) incision was made was 2.8 mm2 (range 1.2–5.3 mm2) [33]. None of the lesions involved the overlying articular cartilage, which is avascular throughout life and does not depend on a blood supply from cartilage canal vessels.

Bottom Line: All relaxation time constants differentiated normal epiphyseal cartilage from lesions of ischemic cartilage necrosis, and the histological staining results confirmed the proteoglycan (PG) loss in the areas of ischemia.In the scanned specimens, all of the measured relaxation time constants were higher in the articular than in the normal epiphyseal cartilage, consistently allowing differentiation between these two tissues.Multiparametric MRI provided a sensitive approach to discriminate between necrotic and viable epiphyseal cartilage and between articular and epiphyseal cartilage, which may be useful for diagnosing and monitoring OC lesions and, potentially, for assessing effectiveness of treatment interventions.

View Article: PubMed Central - PubMed

Affiliation: Center for Magnetic Resonance Research, Department of Radiology, University of Minnesota, Minneapolis, MN, United States of America; Department of Orthopaedic Surgery, University of Minnesota, Minneapolis, MN, United States of America.

ABSTRACT

Purpose: To evaluate multiple MRI parameters in a surgical model of osteochondrosis (OC) in goats.

Methods: Focal ischemic lesions of two different sizes were induced in the epiphyseal cartilage of the medial femoral condyles of goats at 4 days of age by surgical transection of cartilage canal blood vessels. Goats were euthanized and specimens harvested 3, 4, 5, 6, 9 and 10 weeks post-op. Ex vivo MRI scans were conducted at 9.4 Tesla for mapping the T1, T2, T1ρ, adiabatic T1ρ and TRAFF relaxation times of articular cartilage, unaffected epiphyseal cartilage, and epiphyseal cartilage within the area of the induced lesion. After MRI scans, safranin O staining was conducted to validate areas of ischemic necrosis induced in the medial femoral condyles of six goats, and to allow comparison of MRI findings with the semi-quantitative proteoglycan assessment in corresponding safranin O-stained histological sections.

Results: All relaxation time constants differentiated normal epiphyseal cartilage from lesions of ischemic cartilage necrosis, and the histological staining results confirmed the proteoglycan (PG) loss in the areas of ischemia. In the scanned specimens, all of the measured relaxation time constants were higher in the articular than in the normal epiphyseal cartilage, consistently allowing differentiation between these two tissues.

Conclusions: Multiparametric MRI provided a sensitive approach to discriminate between necrotic and viable epiphyseal cartilage and between articular and epiphyseal cartilage, which may be useful for diagnosing and monitoring OC lesions and, potentially, for assessing effectiveness of treatment interventions.

No MeSH data available.


Related in: MedlinePlus