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Molecular and Physiological Characterization of Two Novel Multirepeat β-Thymosins from Silkworm, Bombyx mori.

Ma S, Kang Z, Lü P, Yang Y, Yao Q, Xia H, Chen K - PLoS ONE (2015)

Bottom Line: Amazingly, the expression of BmTHY2 was hugely increased during the pupae stage, indicating a specialized role in this period.The expression of these proteins was gradually decreased in BmN cells infected by BmNPV, suggesting they may play different roles in the virus infection.In addition, both BmTHY1 and BmTHY2 can interact with 14-3-3 of silkworm and Ubiquitin of BmNPV as shown by GST pull down and Co-IP assays, consistent with their roles in the regulation of the development of nervous system.

View Article: PubMed Central - PubMed

Affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, P. R. China.

ABSTRACT
β-thymosin plays important roles in the development of the lymphatic system and the central nervous system in vertebrates. However, its role and function in invertebrates remain much less explored. Here, we firstly isolated a gene encoding β-thymosin in silkworm (Bombyx mori L.). Interestingly, this gene encodes two polypeptides, named as BmTHY1 and BmTHY2, via two different modes of RNA splicing. The recombinant proteins fused with an N-term GST tag were over-expressed in Escherichia coli (E. coli) and further purified to near homogenity to prepare mouse antibodies. The Western blot analysis showed that these proteins were expressed in various tissues and organs, as well as in different developmental stages. Amazingly, the expression of BmTHY2 was hugely increased during the pupae stage, indicating a specialized role in this period. The expression of these proteins was gradually decreased in BmN cells infected by BmNPV, suggesting they may play different roles in the virus infection. In addition, both BmTHY1 and BmTHY2 can interact with 14-3-3 of silkworm and Ubiquitin of BmNPV as shown by GST pull down and Co-IP assays, consistent with their roles in the regulation of the development of nervous system.

No MeSH data available.


Related in: MedlinePlus

The effects BmTHYs on cell proliferation and migration.A: The initial status after scratch. B: 12 hours later, cell grew, the distance narrowed (without any reagents). C: 12 hours later, BmTHY1 efficiently promote cell migrate. D: 12 hours later, BmTHY2 efficiently promote cell migrate. E: MTT assay to detect the effect of BmTHYs on cell proliferation, * P < 0.05. F: Analysis the effect of BmTHYs on cell proliferation, ** P < 0.01.
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pone.0140182.g008: The effects BmTHYs on cell proliferation and migration.A: The initial status after scratch. B: 12 hours later, cell grew, the distance narrowed (without any reagents). C: 12 hours later, BmTHY1 efficiently promote cell migrate. D: 12 hours later, BmTHY2 efficiently promote cell migrate. E: MTT assay to detect the effect of BmTHYs on cell proliferation, * P < 0.05. F: Analysis the effect of BmTHYs on cell proliferation, ** P < 0.01.

Mentions: The gastric cancer cells have been used in previous studies to examine the effect of thymosins on cell proliferation and migration, and thus were also employed in this study. As shown in Fig 8, both BmTHY1 and BmTHY2 could promote the proliferation of gastric cancer cells, and BmTHY1 seemed to have a stronger effect than BmTHY2. In addition, they both could effectively stimulate the migration of gastric cancer cells.


Molecular and Physiological Characterization of Two Novel Multirepeat β-Thymosins from Silkworm, Bombyx mori.

Ma S, Kang Z, Lü P, Yang Y, Yao Q, Xia H, Chen K - PLoS ONE (2015)

The effects BmTHYs on cell proliferation and migration.A: The initial status after scratch. B: 12 hours later, cell grew, the distance narrowed (without any reagents). C: 12 hours later, BmTHY1 efficiently promote cell migrate. D: 12 hours later, BmTHY2 efficiently promote cell migrate. E: MTT assay to detect the effect of BmTHYs on cell proliferation, * P < 0.05. F: Analysis the effect of BmTHYs on cell proliferation, ** P < 0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4608725&req=5

pone.0140182.g008: The effects BmTHYs on cell proliferation and migration.A: The initial status after scratch. B: 12 hours later, cell grew, the distance narrowed (without any reagents). C: 12 hours later, BmTHY1 efficiently promote cell migrate. D: 12 hours later, BmTHY2 efficiently promote cell migrate. E: MTT assay to detect the effect of BmTHYs on cell proliferation, * P < 0.05. F: Analysis the effect of BmTHYs on cell proliferation, ** P < 0.01.
Mentions: The gastric cancer cells have been used in previous studies to examine the effect of thymosins on cell proliferation and migration, and thus were also employed in this study. As shown in Fig 8, both BmTHY1 and BmTHY2 could promote the proliferation of gastric cancer cells, and BmTHY1 seemed to have a stronger effect than BmTHY2. In addition, they both could effectively stimulate the migration of gastric cancer cells.

Bottom Line: Amazingly, the expression of BmTHY2 was hugely increased during the pupae stage, indicating a specialized role in this period.The expression of these proteins was gradually decreased in BmN cells infected by BmNPV, suggesting they may play different roles in the virus infection.In addition, both BmTHY1 and BmTHY2 can interact with 14-3-3 of silkworm and Ubiquitin of BmNPV as shown by GST pull down and Co-IP assays, consistent with their roles in the regulation of the development of nervous system.

View Article: PubMed Central - PubMed

Affiliation: Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu, P. R. China.

ABSTRACT
β-thymosin plays important roles in the development of the lymphatic system and the central nervous system in vertebrates. However, its role and function in invertebrates remain much less explored. Here, we firstly isolated a gene encoding β-thymosin in silkworm (Bombyx mori L.). Interestingly, this gene encodes two polypeptides, named as BmTHY1 and BmTHY2, via two different modes of RNA splicing. The recombinant proteins fused with an N-term GST tag were over-expressed in Escherichia coli (E. coli) and further purified to near homogenity to prepare mouse antibodies. The Western blot analysis showed that these proteins were expressed in various tissues and organs, as well as in different developmental stages. Amazingly, the expression of BmTHY2 was hugely increased during the pupae stage, indicating a specialized role in this period. The expression of these proteins was gradually decreased in BmN cells infected by BmNPV, suggesting they may play different roles in the virus infection. In addition, both BmTHY1 and BmTHY2 can interact with 14-3-3 of silkworm and Ubiquitin of BmNPV as shown by GST pull down and Co-IP assays, consistent with their roles in the regulation of the development of nervous system.

No MeSH data available.


Related in: MedlinePlus