Limits...
Purification and Characterization of Bacteriocin Produced by Weissella confusa A3 of Dairy Origin.

Goh HF, Philip K - PLoS ONE (2015)

Bottom Line: Reduction of activity was shown after treatment with proteinase K, trypsin and peptidase that confirmed the proteinaceous nature of the compound.Bacterial cell treated with the bacteriocin also showed significant morphological changes under transmission electron microscope.No virulence and disease related genes can be detected from the genome of the strain.

View Article: PubMed Central - PubMed

Affiliation: Microbiology Division, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.

ABSTRACT
A dramatic increase in bacterial resistance towards currently available antibiotics has raised worldwide concerns for public health. Therefore, antimicrobial peptides (AMPs) have emerged as a promisingly new group of therapeutic agents for managing infectious diseases. The present investigation focusses on the isolation and purification of a novel bacteriocin from an indigenous sample of cow milk and it's mode of action. The bacteriocin was isolated from Weissella confusa A3 that was isolated from the sample and was shown to have inhibitory activity towards pathogenic bacteria namely Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Micrococcus luteus. The bacteriocin was shown to be heat stable and functioned well at low pH (2 to 6). Reduction of activity was shown after treatment with proteinase K, trypsin and peptidase that confirmed the proteinaceous nature of the compound. MALDI-TOF analysis of the sample gave a mass approximating 2.7 kDa. The membrane of the bacteria was disrupted by the bacteriocin causing SYTOX® green dye to enter the cell and bind to the bacterial DNA giving fluorescence signal. Bacterial cell treated with the bacteriocin also showed significant morphological changes under transmission electron microscope. No virulence and disease related genes can be detected from the genome of the strain.

No MeSH data available.


Related in: MedlinePlus

Bactericidal effect of Bacteriocin A3 on B. cereus.TEM images of B. cereus cells before (a) and after (b & c) treatment with bacteriocin extracted from W. confusa. Bar indicates 1μm for (a & c) and 500nm for (b). Arrows indicate destruction of membrane.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4608715&req=5

pone.0140434.g007: Bactericidal effect of Bacteriocin A3 on B. cereus.TEM images of B. cereus cells before (a) and after (b & c) treatment with bacteriocin extracted from W. confusa. Bar indicates 1μm for (a & c) and 500nm for (b). Arrows indicate destruction of membrane.

Mentions: Bacillus cereus treated with bacteriocin and untreated control was observed under transmission electron microscope. The untreated cell showed complete membrane (Fig 7a) and the bacterial cells treated with bacteriocin shows disruption on the membrane. In Fig 7b, the bacterial cell membrane was sloughed off. Fig 7c showed a pore formation on the cell membrane.


Purification and Characterization of Bacteriocin Produced by Weissella confusa A3 of Dairy Origin.

Goh HF, Philip K - PLoS ONE (2015)

Bactericidal effect of Bacteriocin A3 on B. cereus.TEM images of B. cereus cells before (a) and after (b & c) treatment with bacteriocin extracted from W. confusa. Bar indicates 1μm for (a & c) and 500nm for (b). Arrows indicate destruction of membrane.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4608715&req=5

pone.0140434.g007: Bactericidal effect of Bacteriocin A3 on B. cereus.TEM images of B. cereus cells before (a) and after (b & c) treatment with bacteriocin extracted from W. confusa. Bar indicates 1μm for (a & c) and 500nm for (b). Arrows indicate destruction of membrane.
Mentions: Bacillus cereus treated with bacteriocin and untreated control was observed under transmission electron microscope. The untreated cell showed complete membrane (Fig 7a) and the bacterial cells treated with bacteriocin shows disruption on the membrane. In Fig 7b, the bacterial cell membrane was sloughed off. Fig 7c showed a pore formation on the cell membrane.

Bottom Line: Reduction of activity was shown after treatment with proteinase K, trypsin and peptidase that confirmed the proteinaceous nature of the compound.Bacterial cell treated with the bacteriocin also showed significant morphological changes under transmission electron microscope.No virulence and disease related genes can be detected from the genome of the strain.

View Article: PubMed Central - PubMed

Affiliation: Microbiology Division, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.

ABSTRACT
A dramatic increase in bacterial resistance towards currently available antibiotics has raised worldwide concerns for public health. Therefore, antimicrobial peptides (AMPs) have emerged as a promisingly new group of therapeutic agents for managing infectious diseases. The present investigation focusses on the isolation and purification of a novel bacteriocin from an indigenous sample of cow milk and it's mode of action. The bacteriocin was isolated from Weissella confusa A3 that was isolated from the sample and was shown to have inhibitory activity towards pathogenic bacteria namely Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Micrococcus luteus. The bacteriocin was shown to be heat stable and functioned well at low pH (2 to 6). Reduction of activity was shown after treatment with proteinase K, trypsin and peptidase that confirmed the proteinaceous nature of the compound. MALDI-TOF analysis of the sample gave a mass approximating 2.7 kDa. The membrane of the bacteria was disrupted by the bacteriocin causing SYTOX® green dye to enter the cell and bind to the bacterial DNA giving fluorescence signal. Bacterial cell treated with the bacteriocin also showed significant morphological changes under transmission electron microscope. No virulence and disease related genes can be detected from the genome of the strain.

No MeSH data available.


Related in: MedlinePlus