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The Fatty Acid Profile Analysis of Cyperus laxus Used for Phytoremediation of Soils from Aged Oil Spill-Impacted Sites Revealed That This Is a C18:3 Plant Species.

Rivera Casado NA, Montes Horcasitas Mdel C, Rodríguez Vázquez R, Esparza García FJ, Pérez Vargas J, Ariza Castolo A, Ferrera-Cerrato R, Gómez Guzmán O, Calva Calva G - PLoS ONE (2015)

Bottom Line: In plants from the phytoremediation systems, the total fatty acid contents in the leaf and the corm were negatively affected by the hydrocarbons presence; however, the effect was positive in root.Interestingly, under contaminated conditions, unusual fatty acids such as odd numbered carbons (C15, C17, C21, and C23) and uncommon unsaturated chains (C20:3n6 and C20:4) were produced together with a remarkable quantity of C22:2 and C24:0 chains in the corm and the leaf.These results demonstrate that weathered hydrocarbons may drastically affect the lipidic composition of C. laxus at the fatty acid level, suggesting that this species adjusts the cover lipid composition in its vegetative organs, mainly in roots, in response to the weathered hydrocarbon presence and uptake during the phytoremediation process.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology and Bioengineering, CINVESTAV-IPN, Mexico D. F, México.

ABSTRACT
The effect of recalcitrant hydrocarbons on the fatty acid profile from leaf, basal corm, and roots of Cyperus laxus plants cultivated in greenhouse phytoremediation systems of soils from aged oil spill-impacted sites containing from 16 to 340 g/Kg total hydrocarbons (THC) was assessed to investigate if this is a C18:3 species and if the hydrocarbon removal during the phytoremediation process has a relationship with the fatty acid profile of this plant. The fatty acid profile was specific to each vegetative organ and was strongly affected by the hydrocarbons level in the impacted sites. Leaf extracts of plants from uncontaminated soil produced palmitic acid (C16), octadecanoic acid (C18:0), unsaturated oleic acids (C18:1-C18:3), and unsaturated eichosanoic (C20:2-C20:3) acids with a noticeable absence of the unsaturated hexadecatrienoic acid (C16:3); this finding demonstrates, for the first time, that C. laxus is a C18:3 plant. In plants from the phytoremediation systems, the total fatty acid contents in the leaf and the corm were negatively affected by the hydrocarbons presence; however, the effect was positive in root. Interestingly, under contaminated conditions, unusual fatty acids such as odd numbered carbons (C15, C17, C21, and C23) and uncommon unsaturated chains (C20:3n6 and C20:4) were produced together with a remarkable quantity of C22:2 and C24:0 chains in the corm and the leaf. These results demonstrate that weathered hydrocarbons may drastically affect the lipidic composition of C. laxus at the fatty acid level, suggesting that this species adjusts the cover lipid composition in its vegetative organs, mainly in roots, in response to the weathered hydrocarbon presence and uptake during the phytoremediation process.

No MeSH data available.


Related in: MedlinePlus

Estimated means for the content of each fatty acid in organs of Cyperus laxus.Estimated marginal means for the content of individual fatty acids in leaf, corm, and roots of Cyperus laxus based on predictions using the fitted model (Content = Soil + FAME) established from the phytoremediation treatments of soils from the oil spill-impacted sites.
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pone.0140103.g004: Estimated means for the content of each fatty acid in organs of Cyperus laxus.Estimated marginal means for the content of individual fatty acids in leaf, corm, and roots of Cyperus laxus based on predictions using the fitted model (Content = Soil + FAME) established from the phytoremediation treatments of soils from the oil spill-impacted sites.

Mentions: The effect of the THC level on the fatty acid profile (Fig 2), the fatty acid content (Fig 3 and Table 1), and the cumulative chain length distribution (insets in Fig 3) was different for each organ (Fig 4). As shown in the fatty acid profile in the lipid extracts from the leaf of plants from the phytoremediation system of the soil from the impacted site containing 340 g/Kg THC (Fig 2), unusual fatty acids, such as odd numbered carbon (C15, C17, C21, C23) and uncommon unsaturated chains (C20:3n6 and C20:4) were observed together with a remarkable enhancement of the C22:2 and C24:0 chains in the corm and the leaf of the plants from the contaminated soils. The low level or the absence of hexadecatrienoic acid (C16:3) in leaf of plants from the phytoremediation systems (Fig 3), are consistent with the estimated content of C16:3 fatty acid for leaf of plants from the overall phytoremediation treatments of soils from the oil spill-impacted sites based on predictions using the fit model Content = Soil + FAME (Fig 4). This observation agrees with the above results and confirms that C. laxus is a C18:3 plant.


The Fatty Acid Profile Analysis of Cyperus laxus Used for Phytoremediation of Soils from Aged Oil Spill-Impacted Sites Revealed That This Is a C18:3 Plant Species.

Rivera Casado NA, Montes Horcasitas Mdel C, Rodríguez Vázquez R, Esparza García FJ, Pérez Vargas J, Ariza Castolo A, Ferrera-Cerrato R, Gómez Guzmán O, Calva Calva G - PLoS ONE (2015)

Estimated means for the content of each fatty acid in organs of Cyperus laxus.Estimated marginal means for the content of individual fatty acids in leaf, corm, and roots of Cyperus laxus based on predictions using the fitted model (Content = Soil + FAME) established from the phytoremediation treatments of soils from the oil spill-impacted sites.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4608714&req=5

pone.0140103.g004: Estimated means for the content of each fatty acid in organs of Cyperus laxus.Estimated marginal means for the content of individual fatty acids in leaf, corm, and roots of Cyperus laxus based on predictions using the fitted model (Content = Soil + FAME) established from the phytoremediation treatments of soils from the oil spill-impacted sites.
Mentions: The effect of the THC level on the fatty acid profile (Fig 2), the fatty acid content (Fig 3 and Table 1), and the cumulative chain length distribution (insets in Fig 3) was different for each organ (Fig 4). As shown in the fatty acid profile in the lipid extracts from the leaf of plants from the phytoremediation system of the soil from the impacted site containing 340 g/Kg THC (Fig 2), unusual fatty acids, such as odd numbered carbon (C15, C17, C21, C23) and uncommon unsaturated chains (C20:3n6 and C20:4) were observed together with a remarkable enhancement of the C22:2 and C24:0 chains in the corm and the leaf of the plants from the contaminated soils. The low level or the absence of hexadecatrienoic acid (C16:3) in leaf of plants from the phytoremediation systems (Fig 3), are consistent with the estimated content of C16:3 fatty acid for leaf of plants from the overall phytoremediation treatments of soils from the oil spill-impacted sites based on predictions using the fit model Content = Soil + FAME (Fig 4). This observation agrees with the above results and confirms that C. laxus is a C18:3 plant.

Bottom Line: In plants from the phytoremediation systems, the total fatty acid contents in the leaf and the corm were negatively affected by the hydrocarbons presence; however, the effect was positive in root.Interestingly, under contaminated conditions, unusual fatty acids such as odd numbered carbons (C15, C17, C21, and C23) and uncommon unsaturated chains (C20:3n6 and C20:4) were produced together with a remarkable quantity of C22:2 and C24:0 chains in the corm and the leaf.These results demonstrate that weathered hydrocarbons may drastically affect the lipidic composition of C. laxus at the fatty acid level, suggesting that this species adjusts the cover lipid composition in its vegetative organs, mainly in roots, in response to the weathered hydrocarbon presence and uptake during the phytoremediation process.

View Article: PubMed Central - PubMed

Affiliation: Biotechnology and Bioengineering, CINVESTAV-IPN, Mexico D. F, México.

ABSTRACT
The effect of recalcitrant hydrocarbons on the fatty acid profile from leaf, basal corm, and roots of Cyperus laxus plants cultivated in greenhouse phytoremediation systems of soils from aged oil spill-impacted sites containing from 16 to 340 g/Kg total hydrocarbons (THC) was assessed to investigate if this is a C18:3 species and if the hydrocarbon removal during the phytoremediation process has a relationship with the fatty acid profile of this plant. The fatty acid profile was specific to each vegetative organ and was strongly affected by the hydrocarbons level in the impacted sites. Leaf extracts of plants from uncontaminated soil produced palmitic acid (C16), octadecanoic acid (C18:0), unsaturated oleic acids (C18:1-C18:3), and unsaturated eichosanoic (C20:2-C20:3) acids with a noticeable absence of the unsaturated hexadecatrienoic acid (C16:3); this finding demonstrates, for the first time, that C. laxus is a C18:3 plant. In plants from the phytoremediation systems, the total fatty acid contents in the leaf and the corm were negatively affected by the hydrocarbons presence; however, the effect was positive in root. Interestingly, under contaminated conditions, unusual fatty acids such as odd numbered carbons (C15, C17, C21, and C23) and uncommon unsaturated chains (C20:3n6 and C20:4) were produced together with a remarkable quantity of C22:2 and C24:0 chains in the corm and the leaf. These results demonstrate that weathered hydrocarbons may drastically affect the lipidic composition of C. laxus at the fatty acid level, suggesting that this species adjusts the cover lipid composition in its vegetative organs, mainly in roots, in response to the weathered hydrocarbon presence and uptake during the phytoremediation process.

No MeSH data available.


Related in: MedlinePlus