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Naturally Occurring Stilbenoid TSG Reverses Non-Alcoholic Fatty Liver Diseases via Gut-Liver Axis.

Lin P, Lu J, Wang Y, Gu W, Yu J, Zhao R - PLoS ONE (2015)

Bottom Line: TSG regulated gut microbiota balanced and increased the protein expression of ZO-1 and occludin, which could improve the function of the intestinal mucosal barrier and reduce serum LPS content by about 25%.TSG reduced TL4 levels by 56% and NF-κB expression by 23% relative to the NAFLD model group.This suggests that prevention of NAFLD by TSG in HFD-fed rats is mediated by modulation of the gut microbiota and TLR4/NF-κB pathway, which may alleviate chronic low-grade inflammation by reducing the exogenous antigen load on the host.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan Province, China.

ABSTRACT
The gut-liver axis is largely involved in the development of non-alcoholic fatty liver disease (NAFLD). We investigated whether 2, 3, 5, 4'-tetrahydroxy-stilbene-2-O-β-D-glucoside (TSG) could reverse NAFLD induced by a high-fat diet (HFD) and whether it did so via the gut-liver axis. Results showed that TSG could reduce the accumulation of FFA and it did so by reducing the expression of L-FABP and FATP4. TSG regulated gut microbiota balanced and increased the protein expression of ZO-1 and occludin, which could improve the function of the intestinal mucosal barrier and reduce serum LPS content by about 25%. TSG reduced TL4 levels by 56% and NF-κB expression by 23% relative to the NAFLD model group. This suggests that prevention of NAFLD by TSG in HFD-fed rats is mediated by modulation of the gut microbiota and TLR4/NF-κB pathway, which may alleviate chronic low-grade inflammation by reducing the exogenous antigen load on the host.

No MeSH data available.


Related in: MedlinePlus

TSG and intestinal microbial balance regulation.(A) Relative abundance of Bacteroidetes, Firmicutes and Proteobacteria phylum. One fecal sample from each group in the 1st and 12nd week was chosen for pyrosequencing of V4 regions of 16S rDNA, respectively. Blue bars and red bars represented the relative phylum abundance of male MOD group in the 1st and 12nd week, respectively. Green bars and purple bars represented the relative phylum abundance of male TSG.M group in the 1st and 12nd week, respectively. (B) Top 20 abundance genera at the end of the research. Heat map was showing the abundance of top 20 abundance genera in male CON, MOD and TSG.M group. Genera in Bacteroidetes and Proteobacteria phylum had relative higher abundance, while genera in Firmicutes had relative lower abundance after TSG treatment. (C) Regulations on key genera of TSG. Green, red and yellow bars displayed specific genera abundance in male CON, MOD and TSG.M group at the end of the research.
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pone.0140346.g003: TSG and intestinal microbial balance regulation.(A) Relative abundance of Bacteroidetes, Firmicutes and Proteobacteria phylum. One fecal sample from each group in the 1st and 12nd week was chosen for pyrosequencing of V4 regions of 16S rDNA, respectively. Blue bars and red bars represented the relative phylum abundance of male MOD group in the 1st and 12nd week, respectively. Green bars and purple bars represented the relative phylum abundance of male TSG.M group in the 1st and 12nd week, respectively. (B) Top 20 abundance genera at the end of the research. Heat map was showing the abundance of top 20 abundance genera in male CON, MOD and TSG.M group. Genera in Bacteroidetes and Proteobacteria phylum had relative higher abundance, while genera in Firmicutes had relative lower abundance after TSG treatment. (C) Regulations on key genera of TSG. Green, red and yellow bars displayed specific genera abundance in male CON, MOD and TSG.M group at the end of the research.

Mentions: In this study, HFD was found to increase the relative abundance of Firmicutes and Proteobacteria by 4.60% and 1.03%, respectively, and to reduce the abundance of Bacteroidetes from 75.8% to 56.0% in the male group (Fig 3A). These results indicated that the changes in the intestinal microbial balance may be an environmental factor of obesity and NAFLD [22]. These alterations were reversed by TSG treatment (Fig 3A). Relative abundance of top 20 genera (Fig 3B) showed that the TSG could increase Prevotella, [Prevotella], CF231, and Paraprevotella to normal levels effectively. These genera belong to the Bacteroidetes phylum. TSG, meanwhile, also reduced the relative abundance of some genera of Firmicutes phylum, such as Collinsella, Faecalibacterium, and Coprobacillus (Fig 3C).


Naturally Occurring Stilbenoid TSG Reverses Non-Alcoholic Fatty Liver Diseases via Gut-Liver Axis.

Lin P, Lu J, Wang Y, Gu W, Yu J, Zhao R - PLoS ONE (2015)

TSG and intestinal microbial balance regulation.(A) Relative abundance of Bacteroidetes, Firmicutes and Proteobacteria phylum. One fecal sample from each group in the 1st and 12nd week was chosen for pyrosequencing of V4 regions of 16S rDNA, respectively. Blue bars and red bars represented the relative phylum abundance of male MOD group in the 1st and 12nd week, respectively. Green bars and purple bars represented the relative phylum abundance of male TSG.M group in the 1st and 12nd week, respectively. (B) Top 20 abundance genera at the end of the research. Heat map was showing the abundance of top 20 abundance genera in male CON, MOD and TSG.M group. Genera in Bacteroidetes and Proteobacteria phylum had relative higher abundance, while genera in Firmicutes had relative lower abundance after TSG treatment. (C) Regulations on key genera of TSG. Green, red and yellow bars displayed specific genera abundance in male CON, MOD and TSG.M group at the end of the research.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4608713&req=5

pone.0140346.g003: TSG and intestinal microbial balance regulation.(A) Relative abundance of Bacteroidetes, Firmicutes and Proteobacteria phylum. One fecal sample from each group in the 1st and 12nd week was chosen for pyrosequencing of V4 regions of 16S rDNA, respectively. Blue bars and red bars represented the relative phylum abundance of male MOD group in the 1st and 12nd week, respectively. Green bars and purple bars represented the relative phylum abundance of male TSG.M group in the 1st and 12nd week, respectively. (B) Top 20 abundance genera at the end of the research. Heat map was showing the abundance of top 20 abundance genera in male CON, MOD and TSG.M group. Genera in Bacteroidetes and Proteobacteria phylum had relative higher abundance, while genera in Firmicutes had relative lower abundance after TSG treatment. (C) Regulations on key genera of TSG. Green, red and yellow bars displayed specific genera abundance in male CON, MOD and TSG.M group at the end of the research.
Mentions: In this study, HFD was found to increase the relative abundance of Firmicutes and Proteobacteria by 4.60% and 1.03%, respectively, and to reduce the abundance of Bacteroidetes from 75.8% to 56.0% in the male group (Fig 3A). These results indicated that the changes in the intestinal microbial balance may be an environmental factor of obesity and NAFLD [22]. These alterations were reversed by TSG treatment (Fig 3A). Relative abundance of top 20 genera (Fig 3B) showed that the TSG could increase Prevotella, [Prevotella], CF231, and Paraprevotella to normal levels effectively. These genera belong to the Bacteroidetes phylum. TSG, meanwhile, also reduced the relative abundance of some genera of Firmicutes phylum, such as Collinsella, Faecalibacterium, and Coprobacillus (Fig 3C).

Bottom Line: TSG regulated gut microbiota balanced and increased the protein expression of ZO-1 and occludin, which could improve the function of the intestinal mucosal barrier and reduce serum LPS content by about 25%.TSG reduced TL4 levels by 56% and NF-κB expression by 23% relative to the NAFLD model group.This suggests that prevention of NAFLD by TSG in HFD-fed rats is mediated by modulation of the gut microbiota and TLR4/NF-κB pathway, which may alleviate chronic low-grade inflammation by reducing the exogenous antigen load on the host.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan Province, China.

ABSTRACT
The gut-liver axis is largely involved in the development of non-alcoholic fatty liver disease (NAFLD). We investigated whether 2, 3, 5, 4'-tetrahydroxy-stilbene-2-O-β-D-glucoside (TSG) could reverse NAFLD induced by a high-fat diet (HFD) and whether it did so via the gut-liver axis. Results showed that TSG could reduce the accumulation of FFA and it did so by reducing the expression of L-FABP and FATP4. TSG regulated gut microbiota balanced and increased the protein expression of ZO-1 and occludin, which could improve the function of the intestinal mucosal barrier and reduce serum LPS content by about 25%. TSG reduced TL4 levels by 56% and NF-κB expression by 23% relative to the NAFLD model group. This suggests that prevention of NAFLD by TSG in HFD-fed rats is mediated by modulation of the gut microbiota and TLR4/NF-κB pathway, which may alleviate chronic low-grade inflammation by reducing the exogenous antigen load on the host.

No MeSH data available.


Related in: MedlinePlus