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Naturally Occurring Stilbenoid TSG Reverses Non-Alcoholic Fatty Liver Diseases via Gut-Liver Axis.

Lin P, Lu J, Wang Y, Gu W, Yu J, Zhao R - PLoS ONE (2015)

Bottom Line: TSG regulated gut microbiota balanced and increased the protein expression of ZO-1 and occludin, which could improve the function of the intestinal mucosal barrier and reduce serum LPS content by about 25%.TSG reduced TL4 levels by 56% and NF-κB expression by 23% relative to the NAFLD model group.This suggests that prevention of NAFLD by TSG in HFD-fed rats is mediated by modulation of the gut microbiota and TLR4/NF-κB pathway, which may alleviate chronic low-grade inflammation by reducing the exogenous antigen load on the host.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan Province, China.

ABSTRACT
The gut-liver axis is largely involved in the development of non-alcoholic fatty liver disease (NAFLD). We investigated whether 2, 3, 5, 4'-tetrahydroxy-stilbene-2-O-β-D-glucoside (TSG) could reverse NAFLD induced by a high-fat diet (HFD) and whether it did so via the gut-liver axis. Results showed that TSG could reduce the accumulation of FFA and it did so by reducing the expression of L-FABP and FATP4. TSG regulated gut microbiota balanced and increased the protein expression of ZO-1 and occludin, which could improve the function of the intestinal mucosal barrier and reduce serum LPS content by about 25%. TSG reduced TL4 levels by 56% and NF-κB expression by 23% relative to the NAFLD model group. This suggests that prevention of NAFLD by TSG in HFD-fed rats is mediated by modulation of the gut microbiota and TLR4/NF-κB pathway, which may alleviate chronic low-grade inflammation by reducing the exogenous antigen load on the host.

No MeSH data available.


Related in: MedlinePlus

Hepatic histology and expressions of VLDL, L-FABP and FATP4 in liver tissues.(A) Representative images (200×magnification, haematoxylin and eosin stain) of hepatic histology in CON, MOD and TSG.M groups. No obvious fatty degeneration was observed in hepatocytes of CON group. Obvious edema (yellow cycles) and steatosis (red arrows) were observed in hepatocytes after high fat diet fed for12weeks.These edema and steatosis were markedly relieved in TSG.M group. (B) Concentrations of VLDL, L-FABP, and FATP4 in liver homogenate samples. The bars represented protein levels of VLDL, L-FABP, and FATP4 in liver homogenate samples, which were determined by ELISA in all groups (mean ± SD, n = 7). Higher experssions of VLDL, L-FABP, and FATP4 were found in MOD group due to the high fat feed induction.TSG treatment could significantly reduce their expressions. Statistical significance: * p< 0.05 vs. control;** p< 0.01 vs. control; # p< 0.05 vs. model; ## p< 0.01 vs. mode.
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pone.0140346.g001: Hepatic histology and expressions of VLDL, L-FABP and FATP4 in liver tissues.(A) Representative images (200×magnification, haematoxylin and eosin stain) of hepatic histology in CON, MOD and TSG.M groups. No obvious fatty degeneration was observed in hepatocytes of CON group. Obvious edema (yellow cycles) and steatosis (red arrows) were observed in hepatocytes after high fat diet fed for12weeks.These edema and steatosis were markedly relieved in TSG.M group. (B) Concentrations of VLDL, L-FABP, and FATP4 in liver homogenate samples. The bars represented protein levels of VLDL, L-FABP, and FATP4 in liver homogenate samples, which were determined by ELISA in all groups (mean ± SD, n = 7). Higher experssions of VLDL, L-FABP, and FATP4 were found in MOD group due to the high fat feed induction.TSG treatment could significantly reduce their expressions. Statistical significance: * p< 0.05 vs. control;** p< 0.01 vs. control; # p< 0.05 vs. model; ## p< 0.01 vs. mode.

Mentions: The initial body weights of the male and female rats were similar (215 ± 5 g), and the daily food intake was similar among all groups. There was no difference in the body weight growth rate between normal food feed groups and HFD groups. However, weight growth showed distinct gender differences. TSG treatments did not produce a sharp decline in appetite and weight in rats. The liver index in the HFD group increased significantly (P < 0.01) (Table 1) with obvious edema and steatosis (Fig 1A).


Naturally Occurring Stilbenoid TSG Reverses Non-Alcoholic Fatty Liver Diseases via Gut-Liver Axis.

Lin P, Lu J, Wang Y, Gu W, Yu J, Zhao R - PLoS ONE (2015)

Hepatic histology and expressions of VLDL, L-FABP and FATP4 in liver tissues.(A) Representative images (200×magnification, haematoxylin and eosin stain) of hepatic histology in CON, MOD and TSG.M groups. No obvious fatty degeneration was observed in hepatocytes of CON group. Obvious edema (yellow cycles) and steatosis (red arrows) were observed in hepatocytes after high fat diet fed for12weeks.These edema and steatosis were markedly relieved in TSG.M group. (B) Concentrations of VLDL, L-FABP, and FATP4 in liver homogenate samples. The bars represented protein levels of VLDL, L-FABP, and FATP4 in liver homogenate samples, which were determined by ELISA in all groups (mean ± SD, n = 7). Higher experssions of VLDL, L-FABP, and FATP4 were found in MOD group due to the high fat feed induction.TSG treatment could significantly reduce their expressions. Statistical significance: * p< 0.05 vs. control;** p< 0.01 vs. control; # p< 0.05 vs. model; ## p< 0.01 vs. mode.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4608713&req=5

pone.0140346.g001: Hepatic histology and expressions of VLDL, L-FABP and FATP4 in liver tissues.(A) Representative images (200×magnification, haematoxylin and eosin stain) of hepatic histology in CON, MOD and TSG.M groups. No obvious fatty degeneration was observed in hepatocytes of CON group. Obvious edema (yellow cycles) and steatosis (red arrows) were observed in hepatocytes after high fat diet fed for12weeks.These edema and steatosis were markedly relieved in TSG.M group. (B) Concentrations of VLDL, L-FABP, and FATP4 in liver homogenate samples. The bars represented protein levels of VLDL, L-FABP, and FATP4 in liver homogenate samples, which were determined by ELISA in all groups (mean ± SD, n = 7). Higher experssions of VLDL, L-FABP, and FATP4 were found in MOD group due to the high fat feed induction.TSG treatment could significantly reduce their expressions. Statistical significance: * p< 0.05 vs. control;** p< 0.01 vs. control; # p< 0.05 vs. model; ## p< 0.01 vs. mode.
Mentions: The initial body weights of the male and female rats were similar (215 ± 5 g), and the daily food intake was similar among all groups. There was no difference in the body weight growth rate between normal food feed groups and HFD groups. However, weight growth showed distinct gender differences. TSG treatments did not produce a sharp decline in appetite and weight in rats. The liver index in the HFD group increased significantly (P < 0.01) (Table 1) with obvious edema and steatosis (Fig 1A).

Bottom Line: TSG regulated gut microbiota balanced and increased the protein expression of ZO-1 and occludin, which could improve the function of the intestinal mucosal barrier and reduce serum LPS content by about 25%.TSG reduced TL4 levels by 56% and NF-κB expression by 23% relative to the NAFLD model group.This suggests that prevention of NAFLD by TSG in HFD-fed rats is mediated by modulation of the gut microbiota and TLR4/NF-κB pathway, which may alleviate chronic low-grade inflammation by reducing the exogenous antigen load on the host.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacy, Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan Province, China.

ABSTRACT
The gut-liver axis is largely involved in the development of non-alcoholic fatty liver disease (NAFLD). We investigated whether 2, 3, 5, 4'-tetrahydroxy-stilbene-2-O-β-D-glucoside (TSG) could reverse NAFLD induced by a high-fat diet (HFD) and whether it did so via the gut-liver axis. Results showed that TSG could reduce the accumulation of FFA and it did so by reducing the expression of L-FABP and FATP4. TSG regulated gut microbiota balanced and increased the protein expression of ZO-1 and occludin, which could improve the function of the intestinal mucosal barrier and reduce serum LPS content by about 25%. TSG reduced TL4 levels by 56% and NF-κB expression by 23% relative to the NAFLD model group. This suggests that prevention of NAFLD by TSG in HFD-fed rats is mediated by modulation of the gut microbiota and TLR4/NF-κB pathway, which may alleviate chronic low-grade inflammation by reducing the exogenous antigen load on the host.

No MeSH data available.


Related in: MedlinePlus