Limits...
Antiviral Activity of Gold/Copper Sulfide Core/Shell Nanoparticles against Human Norovirus Virus-Like Particles.

Broglie JJ, Alston B, Yang C, Ma L, Adcock AF, Chen W, Yang L - PLoS ONE (2015)

Bottom Line: Increasing nanoparticle concentration and/or VLP-NP contact time significantly increased the virucidal efficacy of Au/CuS NPs.Changes to the VLP particle morphology, size, and capsid protein were characterized using dynamic light scattering, transmission electron microscopy, and Western blot analysis.The strategy reported here provides the first reported proof-of-concept Au/CuS NPs-based virucide for rapidly inactivating human norovirus.

View Article: PubMed Central - PubMed

Affiliation: Biomanufacturing Research Institute and Technology Enterprise (BRITE), Department of Pharmaceutical Sciences, North Carolina Central University, Durham, North Carolina, United States of America.

ABSTRACT
Human norovirus is a leading cause of acute gastroenteritis worldwide in a plethora of residential and commercial settings, including restaurants, schools, and hospitals. Methods for easily detecting the virus and for treating and preventing infection are critical to stopping norovirus outbreaks, and inactivation via nanoparticles (NPs) is a more universal and attractive alternative to other physical and chemical approaches. Using norovirus GI.1 (Norwalk) virus-like particles (VLPs) as a model viral system, this study characterized the antiviral activity of Au/CuS core/shell nanoparticles (NPs) against GI.1 VLPs for the rapid inactivation of HuNoV. Inactivation of VLPs (GI.1) by Au/CuS NPs evaluated using an absorbance-based ELISA indicated that treatment with 0.083 μM NPs for 10 min inactivated ~50% VLPs in a 0.37 μg/ml VLP solution and 0.83 μM NPs for 10 min completely inactivated the VLPs. Increasing nanoparticle concentration and/or VLP-NP contact time significantly increased the virucidal efficacy of Au/CuS NPs. Changes to the VLP particle morphology, size, and capsid protein were characterized using dynamic light scattering, transmission electron microscopy, and Western blot analysis. The strategy reported here provides the first reported proof-of-concept Au/CuS NPs-based virucide for rapidly inactivating human norovirus.

No MeSH data available.


Related in: MedlinePlus

Effect of treatment time on absorbance for VLP solutions treated with 0.83 μM Au/CuS NP.Absorbance was measured using the three-hour ELISA, and new VLP and NP solutions were prepared for each time point. Reduced absorbance suggests structural damage to the capsid surface proteins and is indicative of VLP inactivation.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4608711&req=5

pone.0141050.g002: Effect of treatment time on absorbance for VLP solutions treated with 0.83 μM Au/CuS NP.Absorbance was measured using the three-hour ELISA, and new VLP and NP solutions were prepared for each time point. Reduced absorbance suggests structural damage to the capsid surface proteins and is indicative of VLP inactivation.

Mentions: It turned out that the treatment time had a strong effect on VLP inactivation. Fig 2 shows the absorbance value reductions in VLPs detection by ELISA upon treatment with 0.083 μM Au/CuS for various time periods ranging form 10 min to 4 h. After only 10 and 30 min of treatment, the average absorbance of the treated VLP solutions changes by 35% and 79%, respectively, when compared to the corresponding control solutions, indicating the inactivation of VLPs by Au/CuS NPs was effective and rapid. Also, no absorbance values can be detected for the longer treatment times, while the untreated solutions have similar absorbance values, regardless of treatment time.


Antiviral Activity of Gold/Copper Sulfide Core/Shell Nanoparticles against Human Norovirus Virus-Like Particles.

Broglie JJ, Alston B, Yang C, Ma L, Adcock AF, Chen W, Yang L - PLoS ONE (2015)

Effect of treatment time on absorbance for VLP solutions treated with 0.83 μM Au/CuS NP.Absorbance was measured using the three-hour ELISA, and new VLP and NP solutions were prepared for each time point. Reduced absorbance suggests structural damage to the capsid surface proteins and is indicative of VLP inactivation.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4608711&req=5

pone.0141050.g002: Effect of treatment time on absorbance for VLP solutions treated with 0.83 μM Au/CuS NP.Absorbance was measured using the three-hour ELISA, and new VLP and NP solutions were prepared for each time point. Reduced absorbance suggests structural damage to the capsid surface proteins and is indicative of VLP inactivation.
Mentions: It turned out that the treatment time had a strong effect on VLP inactivation. Fig 2 shows the absorbance value reductions in VLPs detection by ELISA upon treatment with 0.083 μM Au/CuS for various time periods ranging form 10 min to 4 h. After only 10 and 30 min of treatment, the average absorbance of the treated VLP solutions changes by 35% and 79%, respectively, when compared to the corresponding control solutions, indicating the inactivation of VLPs by Au/CuS NPs was effective and rapid. Also, no absorbance values can be detected for the longer treatment times, while the untreated solutions have similar absorbance values, regardless of treatment time.

Bottom Line: Increasing nanoparticle concentration and/or VLP-NP contact time significantly increased the virucidal efficacy of Au/CuS NPs.Changes to the VLP particle morphology, size, and capsid protein were characterized using dynamic light scattering, transmission electron microscopy, and Western blot analysis.The strategy reported here provides the first reported proof-of-concept Au/CuS NPs-based virucide for rapidly inactivating human norovirus.

View Article: PubMed Central - PubMed

Affiliation: Biomanufacturing Research Institute and Technology Enterprise (BRITE), Department of Pharmaceutical Sciences, North Carolina Central University, Durham, North Carolina, United States of America.

ABSTRACT
Human norovirus is a leading cause of acute gastroenteritis worldwide in a plethora of residential and commercial settings, including restaurants, schools, and hospitals. Methods for easily detecting the virus and for treating and preventing infection are critical to stopping norovirus outbreaks, and inactivation via nanoparticles (NPs) is a more universal and attractive alternative to other physical and chemical approaches. Using norovirus GI.1 (Norwalk) virus-like particles (VLPs) as a model viral system, this study characterized the antiviral activity of Au/CuS core/shell nanoparticles (NPs) against GI.1 VLPs for the rapid inactivation of HuNoV. Inactivation of VLPs (GI.1) by Au/CuS NPs evaluated using an absorbance-based ELISA indicated that treatment with 0.083 μM NPs for 10 min inactivated ~50% VLPs in a 0.37 μg/ml VLP solution and 0.83 μM NPs for 10 min completely inactivated the VLPs. Increasing nanoparticle concentration and/or VLP-NP contact time significantly increased the virucidal efficacy of Au/CuS NPs. Changes to the VLP particle morphology, size, and capsid protein were characterized using dynamic light scattering, transmission electron microscopy, and Western blot analysis. The strategy reported here provides the first reported proof-of-concept Au/CuS NPs-based virucide for rapidly inactivating human norovirus.

No MeSH data available.


Related in: MedlinePlus