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Food Deprivation Affects the miRNome in the Lactating Goat Mammary Gland.

Mobuchon L, Marthey S, Le Guillou S, Laloë D, Le Provost F, Leroux C - PLoS ONE (2015)

Bottom Line: Among the putative targets, 19 were previously identified as differently expressed genes (DEG).The functions of these 19 DEG revealed, notably, their involvement in tissue remodelling.In conclusion, this study offers the first evidence of nutriregulated miRNA in the ruminant mammary gland.

View Article: PubMed Central - PubMed

Affiliation: INRA, UMR1313 Génétique Animale et Biologie Intégrative, F-78350, Jouy-en-Josas, France; INRA, UMR1213 Herbivores, F-63122, Saint Genès Champanelle, France; Clermont Université, VetAgro Sup, UMR1213 Herbivores, BP 10448, F-63000, Clermont-Ferrand, France.

ABSTRACT

Background: Nutrition affects milk composition thus influencing its nutritional properties. Nutrition also modifies the expression of mammary genes, whose regulation is not fully understood. MicroRNAs (miRNA) are small non coding RNA which are important post-transcriptional regulators of gene expression by targeting messenger RNAs. Our goal was to characterize miRNA whose expression is regulated by nutrition in the lactating goat mammary gland, which may provide clues to deciphering regulations of the biosynthesis and secretion of milk components.

Methodology/principal findings: Using high-throughput sequencing technology, miRNomes of the lactating mammary gland were established from lactating goats fed ad libitum or deprived of food for 48 h affecting milk production and composition. High throughput miRNA sequencing revealed 30 miRNA with an expression potentially modulated by food deprivation; 16 were down-regulated and 14 were up-regulated. Diana-microT predictive tools suggested a potential role for several nutriregulated miRNA in lipid metabolism. Among the putative targets, 19 were previously identified as differently expressed genes (DEG). The functions of these 19 DEG revealed, notably, their involvement in tissue remodelling.

Conclusion/significance: In conclusion, this study offers the first evidence of nutriregulated miRNA in the ruminant mammary gland. Characterization of these 30 miRNA could contribute to a clearer understanding of gene regulation in the mammary gland in response to nutrition.

No MeSH data available.


Principal component analyses of miRNA in Control and FD goats.L0X: represent 1 library, FD: Food deprived. Analyses were performed on miRNA for libraries according to the diet received by the goats. The two different diets are labelled within their 95% inertia ellipse (Red: Control; Green: FD).
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pone.0140111.g001: Principal component analyses of miRNA in Control and FD goats.L0X: represent 1 library, FD: Food deprived. Analyses were performed on miRNA for libraries according to the diet received by the goats. The two different diets are labelled within their 95% inertia ellipse (Red: Control; Green: FD).

Mentions: By performing expression pattern analysis using PCA, the ten libraries appeared to be clearly discriminated according to the treatments applied to the goats (Fig 1).


Food Deprivation Affects the miRNome in the Lactating Goat Mammary Gland.

Mobuchon L, Marthey S, Le Guillou S, Laloë D, Le Provost F, Leroux C - PLoS ONE (2015)

Principal component analyses of miRNA in Control and FD goats.L0X: represent 1 library, FD: Food deprived. Analyses were performed on miRNA for libraries according to the diet received by the goats. The two different diets are labelled within their 95% inertia ellipse (Red: Control; Green: FD).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4608672&req=5

pone.0140111.g001: Principal component analyses of miRNA in Control and FD goats.L0X: represent 1 library, FD: Food deprived. Analyses were performed on miRNA for libraries according to the diet received by the goats. The two different diets are labelled within their 95% inertia ellipse (Red: Control; Green: FD).
Mentions: By performing expression pattern analysis using PCA, the ten libraries appeared to be clearly discriminated according to the treatments applied to the goats (Fig 1).

Bottom Line: Among the putative targets, 19 were previously identified as differently expressed genes (DEG).The functions of these 19 DEG revealed, notably, their involvement in tissue remodelling.In conclusion, this study offers the first evidence of nutriregulated miRNA in the ruminant mammary gland.

View Article: PubMed Central - PubMed

Affiliation: INRA, UMR1313 Génétique Animale et Biologie Intégrative, F-78350, Jouy-en-Josas, France; INRA, UMR1213 Herbivores, F-63122, Saint Genès Champanelle, France; Clermont Université, VetAgro Sup, UMR1213 Herbivores, BP 10448, F-63000, Clermont-Ferrand, France.

ABSTRACT

Background: Nutrition affects milk composition thus influencing its nutritional properties. Nutrition also modifies the expression of mammary genes, whose regulation is not fully understood. MicroRNAs (miRNA) are small non coding RNA which are important post-transcriptional regulators of gene expression by targeting messenger RNAs. Our goal was to characterize miRNA whose expression is regulated by nutrition in the lactating goat mammary gland, which may provide clues to deciphering regulations of the biosynthesis and secretion of milk components.

Methodology/principal findings: Using high-throughput sequencing technology, miRNomes of the lactating mammary gland were established from lactating goats fed ad libitum or deprived of food for 48 h affecting milk production and composition. High throughput miRNA sequencing revealed 30 miRNA with an expression potentially modulated by food deprivation; 16 were down-regulated and 14 were up-regulated. Diana-microT predictive tools suggested a potential role for several nutriregulated miRNA in lipid metabolism. Among the putative targets, 19 were previously identified as differently expressed genes (DEG). The functions of these 19 DEG revealed, notably, their involvement in tissue remodelling.

Conclusion/significance: In conclusion, this study offers the first evidence of nutriregulated miRNA in the ruminant mammary gland. Characterization of these 30 miRNA could contribute to a clearer understanding of gene regulation in the mammary gland in response to nutrition.

No MeSH data available.