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The Correlation Relationship between P14ARF Gene DNA Methylation and Primary Liver Cancer.

Zhang H, Nie W, Huang F - Med. Sci. Monit. (2015)

Bottom Line: It was found that P14ARF methylation is associated with the degree of malignancy in multiple tumors.It did not present an obvious difference in tumors with different size.Compared with the adjacent tissue, P14ARF mRNA in carcinoma tissue decreased by 31% (t=28.91, P<0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Third Xiangya Hospital, Central South University, Changsha, Hunan, China (mainland).

ABSTRACT

Background: Primary liver cancer is a common malignant tumor that causes serious damage to human health. DNA methylation is common in epigenetics. DNA methylation plays an important role in the process of primary liver cancer occurrence and development. The P14ARF gene is an important tumor suppressor gene. It was found that P14ARF methylation is associated with the degree of malignancy in multiple tumors. Therefore, this study aimed to investigate the relationship between P14ARF methylation level and primary liver cancer malignant degree.

Material and methods: Carcinoma tissues and adjacent tissues were collected from 87 primary liver cancer patients. Pyrosequencing was applied to obtain P14ARF methylation. Real-time PCR was used to detect P14ARF mRNA level.

Results: P14ARF methylation level in cancerous tissue was significantly higher than in the adjacent tissue (t=76.54, P<0.001). P14ARF methylation showed no significant difference in patients with different age, sex, smoking status, or drinking status. It did not present an obvious difference in tumors with different size. Its methylation level increased following the improvement of TNM stage (P<0.05). Compared with the adjacent tissue, P14ARF mRNA in carcinoma tissue decreased by 31% (t=28.91, P<0.001). P14ARF methylation showed a significant negative correlation with mRNA expression in cancerous tissue (r=-0.43, P<0.01).

Conclusions: P14ARF mRNA level is regulated by DNA methylation in primary liver cancer. P14ARF gene DNA methylation may be associated with the occurrence of primary liver cancer occurrence and TNM staging.

No MeSH data available.


Related in: MedlinePlus

P14ARF gene mRNA expression (A) and DNA methylation level (B) in patients with different TNM stages. * p<0.01 compared with stage I.
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f1-medscimonit-21-3077: P14ARF gene mRNA expression (A) and DNA methylation level (B) in patients with different TNM stages. * p<0.01 compared with stage I.

Mentions: Real-time PCR was used to detect P14ARF mRNA expression level (Figure 1). Compared with the adjacent tissue, P14ARF mRNA expression decreased by about 31% in carcinoma tissue (t=28.91, P<0.001). One-way ANOVA results revealed that P14ARF mRNA level was different in patients with different TNM stage (F=24.15, P<0.001). Linear regression analysis suggested that its level reduced gradually following TNM staging increase (P<0.05).


The Correlation Relationship between P14ARF Gene DNA Methylation and Primary Liver Cancer.

Zhang H, Nie W, Huang F - Med. Sci. Monit. (2015)

P14ARF gene mRNA expression (A) and DNA methylation level (B) in patients with different TNM stages. * p<0.01 compared with stage I.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4608642&req=5

f1-medscimonit-21-3077: P14ARF gene mRNA expression (A) and DNA methylation level (B) in patients with different TNM stages. * p<0.01 compared with stage I.
Mentions: Real-time PCR was used to detect P14ARF mRNA expression level (Figure 1). Compared with the adjacent tissue, P14ARF mRNA expression decreased by about 31% in carcinoma tissue (t=28.91, P<0.001). One-way ANOVA results revealed that P14ARF mRNA level was different in patients with different TNM stage (F=24.15, P<0.001). Linear regression analysis suggested that its level reduced gradually following TNM staging increase (P<0.05).

Bottom Line: It was found that P14ARF methylation is associated with the degree of malignancy in multiple tumors.It did not present an obvious difference in tumors with different size.Compared with the adjacent tissue, P14ARF mRNA in carcinoma tissue decreased by 31% (t=28.91, P<0.001).

View Article: PubMed Central - PubMed

Affiliation: Department of General Surgery, Third Xiangya Hospital, Central South University, Changsha, Hunan, China (mainland).

ABSTRACT

Background: Primary liver cancer is a common malignant tumor that causes serious damage to human health. DNA methylation is common in epigenetics. DNA methylation plays an important role in the process of primary liver cancer occurrence and development. The P14ARF gene is an important tumor suppressor gene. It was found that P14ARF methylation is associated with the degree of malignancy in multiple tumors. Therefore, this study aimed to investigate the relationship between P14ARF methylation level and primary liver cancer malignant degree.

Material and methods: Carcinoma tissues and adjacent tissues were collected from 87 primary liver cancer patients. Pyrosequencing was applied to obtain P14ARF methylation. Real-time PCR was used to detect P14ARF mRNA level.

Results: P14ARF methylation level in cancerous tissue was significantly higher than in the adjacent tissue (t=76.54, P<0.001). P14ARF methylation showed no significant difference in patients with different age, sex, smoking status, or drinking status. It did not present an obvious difference in tumors with different size. Its methylation level increased following the improvement of TNM stage (P<0.05). Compared with the adjacent tissue, P14ARF mRNA in carcinoma tissue decreased by 31% (t=28.91, P<0.001). P14ARF methylation showed a significant negative correlation with mRNA expression in cancerous tissue (r=-0.43, P<0.01).

Conclusions: P14ARF mRNA level is regulated by DNA methylation in primary liver cancer. P14ARF gene DNA methylation may be associated with the occurrence of primary liver cancer occurrence and TNM staging.

No MeSH data available.


Related in: MedlinePlus