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n-Butanol extract from Folium isatidis inhibits lipopolysaccharide-induced inflammatory cytokine production in macrophages and protects mice against lipopolysaccharide-induced endotoxic shock.

Jiang L, Lu Y, Jin J, Dong L, Xu F, Chen S, Wang Z, Liang G, Shan X - Drug Des Devel Ther (2015)

Bottom Line: Studies have shown beneficial pharmacological effects for Folium isatidis.The present study further illuminated the effects of n-butanol extract from Folium isatidis in LPS-induced septic shock and identified the main active chemical components.Additionally, the extract exhibited dramatic protective effects against lung injury and death in mice with septic shock.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, People's Republic of China.

ABSTRACT
Sepsis, which is caused by severe infection, is an important cause of mortality, but effective clinical treatment against sepsis is extremely limited. As the main component of the outer membrane of Gram-negative bacteria, lipopolysaccharide (LPS) plays a major role in inflammatory responses. Studies have shown beneficial pharmacological effects for Folium isatidis. The present study further illuminated the effects of n-butanol extract from Folium isatidis in LPS-induced septic shock and identified the main active chemical components. Our study showed that pretreatment with n-butanol extract from Folium isatidis not only significantly inhibited LPS-induced tumor necrosis factor-α and interleukin-6 production but also markedly and dose dependently enhanced the recruitment of MyD88, the phosphorylation of extracellular signal-regulated kinase, and the degradation of IκB-α. Additionally, the extract exhibited dramatic protective effects against lung injury and death in mice with septic shock. Eight main active compounds were identified, including organic acids, glycoside, indolinones, and flavonoids. These findings provide a perspective on the respiratory protection offered by n-butanol extract from Folium isatidis in LPS-induced sepsis and outline a novel therapeutic strategy for the treatment of sepsis.

No MeSH data available.


Related in: MedlinePlus

The chromatograms for the n-butanol extract from Folium isatidis.Notes: (A) Anthranilic acid, (B) adenosine, (C) pyroglutamic acid, (D) 4(3H)-quinazolinone, (E) 5-hydroxy-2-molindone, (F) nicotinic acid, (G) isoscoparin, and (H) isovitexin.Abbreviation: RT, retention time.
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f5-dddt-9-5601: The chromatograms for the n-butanol extract from Folium isatidis.Notes: (A) Anthranilic acid, (B) adenosine, (C) pyroglutamic acid, (D) 4(3H)-quinazolinone, (E) 5-hydroxy-2-molindone, (F) nicotinic acid, (G) isoscoparin, and (H) isovitexin.Abbreviation: RT, retention time.

Mentions: Extraction of the n-butanol fraction was successfully partitioned from air-dried and pulverized Folium isatidis by successively using petroleum ether, ethyl acetate, and n-butanol. By selecting the optimal experimental conditions for LC–MS/MS, the major active ingredients of the n-butanol extract obtained from Folium isatidis were well separated and effectively analyzed. Figure 5 shows the chromatograms of the n-butanol extract from Folium isatidis. Eight main active ingredients and structures were identified in the n-butanol extract by comparing the tandem mass spectral data and major fragment ions with those described in the related literature, as shown in Table 1 and Figure 6. Among the eight investigated constituents, compounds A, C, and F are organic acids; compound B is a glycoside; compounds D and E are indolinones; and compounds G and H are flavonoids. Additionally, the major active ingredients of the n-butanol extract obtained from Folium isatidis were well separated and effectively analyzed, but the compounds that dominate as anti-inflammatory agents and whether they have orchestrated effects remain to be further evaluated.


n-Butanol extract from Folium isatidis inhibits lipopolysaccharide-induced inflammatory cytokine production in macrophages and protects mice against lipopolysaccharide-induced endotoxic shock.

Jiang L, Lu Y, Jin J, Dong L, Xu F, Chen S, Wang Z, Liang G, Shan X - Drug Des Devel Ther (2015)

The chromatograms for the n-butanol extract from Folium isatidis.Notes: (A) Anthranilic acid, (B) adenosine, (C) pyroglutamic acid, (D) 4(3H)-quinazolinone, (E) 5-hydroxy-2-molindone, (F) nicotinic acid, (G) isoscoparin, and (H) isovitexin.Abbreviation: RT, retention time.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4608600&req=5

f5-dddt-9-5601: The chromatograms for the n-butanol extract from Folium isatidis.Notes: (A) Anthranilic acid, (B) adenosine, (C) pyroglutamic acid, (D) 4(3H)-quinazolinone, (E) 5-hydroxy-2-molindone, (F) nicotinic acid, (G) isoscoparin, and (H) isovitexin.Abbreviation: RT, retention time.
Mentions: Extraction of the n-butanol fraction was successfully partitioned from air-dried and pulverized Folium isatidis by successively using petroleum ether, ethyl acetate, and n-butanol. By selecting the optimal experimental conditions for LC–MS/MS, the major active ingredients of the n-butanol extract obtained from Folium isatidis were well separated and effectively analyzed. Figure 5 shows the chromatograms of the n-butanol extract from Folium isatidis. Eight main active ingredients and structures were identified in the n-butanol extract by comparing the tandem mass spectral data and major fragment ions with those described in the related literature, as shown in Table 1 and Figure 6. Among the eight investigated constituents, compounds A, C, and F are organic acids; compound B is a glycoside; compounds D and E are indolinones; and compounds G and H are flavonoids. Additionally, the major active ingredients of the n-butanol extract obtained from Folium isatidis were well separated and effectively analyzed, but the compounds that dominate as anti-inflammatory agents and whether they have orchestrated effects remain to be further evaluated.

Bottom Line: Studies have shown beneficial pharmacological effects for Folium isatidis.The present study further illuminated the effects of n-butanol extract from Folium isatidis in LPS-induced septic shock and identified the main active chemical components.Additionally, the extract exhibited dramatic protective effects against lung injury and death in mice with septic shock.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, People's Republic of China.

ABSTRACT
Sepsis, which is caused by severe infection, is an important cause of mortality, but effective clinical treatment against sepsis is extremely limited. As the main component of the outer membrane of Gram-negative bacteria, lipopolysaccharide (LPS) plays a major role in inflammatory responses. Studies have shown beneficial pharmacological effects for Folium isatidis. The present study further illuminated the effects of n-butanol extract from Folium isatidis in LPS-induced septic shock and identified the main active chemical components. Our study showed that pretreatment with n-butanol extract from Folium isatidis not only significantly inhibited LPS-induced tumor necrosis factor-α and interleukin-6 production but also markedly and dose dependently enhanced the recruitment of MyD88, the phosphorylation of extracellular signal-regulated kinase, and the degradation of IκB-α. Additionally, the extract exhibited dramatic protective effects against lung injury and death in mice with septic shock. Eight main active compounds were identified, including organic acids, glycoside, indolinones, and flavonoids. These findings provide a perspective on the respiratory protection offered by n-butanol extract from Folium isatidis in LPS-induced sepsis and outline a novel therapeutic strategy for the treatment of sepsis.

No MeSH data available.


Related in: MedlinePlus