Limits...
n-Butanol extract from Folium isatidis inhibits lipopolysaccharide-induced inflammatory cytokine production in macrophages and protects mice against lipopolysaccharide-induced endotoxic shock.

Jiang L, Lu Y, Jin J, Dong L, Xu F, Chen S, Wang Z, Liang G, Shan X - Drug Des Devel Ther (2015)

Bottom Line: Studies have shown beneficial pharmacological effects for Folium isatidis.The present study further illuminated the effects of n-butanol extract from Folium isatidis in LPS-induced septic shock and identified the main active chemical components.Additionally, the extract exhibited dramatic protective effects against lung injury and death in mice with septic shock.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, People's Republic of China.

ABSTRACT
Sepsis, which is caused by severe infection, is an important cause of mortality, but effective clinical treatment against sepsis is extremely limited. As the main component of the outer membrane of Gram-negative bacteria, lipopolysaccharide (LPS) plays a major role in inflammatory responses. Studies have shown beneficial pharmacological effects for Folium isatidis. The present study further illuminated the effects of n-butanol extract from Folium isatidis in LPS-induced septic shock and identified the main active chemical components. Our study showed that pretreatment with n-butanol extract from Folium isatidis not only significantly inhibited LPS-induced tumor necrosis factor-α and interleukin-6 production but also markedly and dose dependently enhanced the recruitment of MyD88, the phosphorylation of extracellular signal-regulated kinase, and the degradation of IκB-α. Additionally, the extract exhibited dramatic protective effects against lung injury and death in mice with septic shock. Eight main active compounds were identified, including organic acids, glycoside, indolinones, and flavonoids. These findings provide a perspective on the respiratory protection offered by n-butanol extract from Folium isatidis in LPS-induced sepsis and outline a novel therapeutic strategy for the treatment of sepsis.

No MeSH data available.


Related in: MedlinePlus

The n-butanol extract from Folium isatidis effectively protected mice from LPS-induced septic shock.Notes: C57BL/6 mice (n=10 per group) were treated with the extract at a dosage of 5 g/kg/day for 7 days before LPS (20 mg/kg or 10 mg/kg, iv) administration. Survival rates were monitored for 7 days after LPS (20 mg/kg) injection (A). Four hours after LPS (10 mg/kg) injection, serum was collected for TNF-α and IL-6 evaluation (B and C), and histological changes in the lungs were analyzed using H&E staining (200×) (D). Statistical significance compared with the LPS group is indicated, *P<0.02, **P<0.01.Abbreviations: IL, interleukin; iv, intravenous; LPS, lipopolysaccharide; TNF-α, tumor necrosis factor-α.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4608600&req=5

f4-dddt-9-5601: The n-butanol extract from Folium isatidis effectively protected mice from LPS-induced septic shock.Notes: C57BL/6 mice (n=10 per group) were treated with the extract at a dosage of 5 g/kg/day for 7 days before LPS (20 mg/kg or 10 mg/kg, iv) administration. Survival rates were monitored for 7 days after LPS (20 mg/kg) injection (A). Four hours after LPS (10 mg/kg) injection, serum was collected for TNF-α and IL-6 evaluation (B and C), and histological changes in the lungs were analyzed using H&E staining (200×) (D). Statistical significance compared with the LPS group is indicated, *P<0.02, **P<0.01.Abbreviations: IL, interleukin; iv, intravenous; LPS, lipopolysaccharide; TNF-α, tumor necrosis factor-α.

Mentions: The n-butanol extract obtained from Folium isatidis exhibited significant effects in vitro, thus prompting us to investigate the effect of the extract on LPS-induced endotoxic shock in vivo. C57BL/6 mice were treated with the extract at a dosage of 5 g/kg/day for 7 consecutive days. All mice were then challenged with LPS (20 mg/kg, intravenous), and survival rates were monitored for 7 days. We compared the survival rates between the extract-treated and the LPS control mice. The extract significantly improved the survival rate of mice with LPS-induced sepsis (Figure 4A).


n-Butanol extract from Folium isatidis inhibits lipopolysaccharide-induced inflammatory cytokine production in macrophages and protects mice against lipopolysaccharide-induced endotoxic shock.

Jiang L, Lu Y, Jin J, Dong L, Xu F, Chen S, Wang Z, Liang G, Shan X - Drug Des Devel Ther (2015)

The n-butanol extract from Folium isatidis effectively protected mice from LPS-induced septic shock.Notes: C57BL/6 mice (n=10 per group) were treated with the extract at a dosage of 5 g/kg/day for 7 days before LPS (20 mg/kg or 10 mg/kg, iv) administration. Survival rates were monitored for 7 days after LPS (20 mg/kg) injection (A). Four hours after LPS (10 mg/kg) injection, serum was collected for TNF-α and IL-6 evaluation (B and C), and histological changes in the lungs were analyzed using H&E staining (200×) (D). Statistical significance compared with the LPS group is indicated, *P<0.02, **P<0.01.Abbreviations: IL, interleukin; iv, intravenous; LPS, lipopolysaccharide; TNF-α, tumor necrosis factor-α.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4608600&req=5

f4-dddt-9-5601: The n-butanol extract from Folium isatidis effectively protected mice from LPS-induced septic shock.Notes: C57BL/6 mice (n=10 per group) were treated with the extract at a dosage of 5 g/kg/day for 7 days before LPS (20 mg/kg or 10 mg/kg, iv) administration. Survival rates were monitored for 7 days after LPS (20 mg/kg) injection (A). Four hours after LPS (10 mg/kg) injection, serum was collected for TNF-α and IL-6 evaluation (B and C), and histological changes in the lungs were analyzed using H&E staining (200×) (D). Statistical significance compared with the LPS group is indicated, *P<0.02, **P<0.01.Abbreviations: IL, interleukin; iv, intravenous; LPS, lipopolysaccharide; TNF-α, tumor necrosis factor-α.
Mentions: The n-butanol extract obtained from Folium isatidis exhibited significant effects in vitro, thus prompting us to investigate the effect of the extract on LPS-induced endotoxic shock in vivo. C57BL/6 mice were treated with the extract at a dosage of 5 g/kg/day for 7 consecutive days. All mice were then challenged with LPS (20 mg/kg, intravenous), and survival rates were monitored for 7 days. We compared the survival rates between the extract-treated and the LPS control mice. The extract significantly improved the survival rate of mice with LPS-induced sepsis (Figure 4A).

Bottom Line: Studies have shown beneficial pharmacological effects for Folium isatidis.The present study further illuminated the effects of n-butanol extract from Folium isatidis in LPS-induced septic shock and identified the main active chemical components.Additionally, the extract exhibited dramatic protective effects against lung injury and death in mice with septic shock.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, People's Republic of China.

ABSTRACT
Sepsis, which is caused by severe infection, is an important cause of mortality, but effective clinical treatment against sepsis is extremely limited. As the main component of the outer membrane of Gram-negative bacteria, lipopolysaccharide (LPS) plays a major role in inflammatory responses. Studies have shown beneficial pharmacological effects for Folium isatidis. The present study further illuminated the effects of n-butanol extract from Folium isatidis in LPS-induced septic shock and identified the main active chemical components. Our study showed that pretreatment with n-butanol extract from Folium isatidis not only significantly inhibited LPS-induced tumor necrosis factor-α and interleukin-6 production but also markedly and dose dependently enhanced the recruitment of MyD88, the phosphorylation of extracellular signal-regulated kinase, and the degradation of IκB-α. Additionally, the extract exhibited dramatic protective effects against lung injury and death in mice with septic shock. Eight main active compounds were identified, including organic acids, glycoside, indolinones, and flavonoids. These findings provide a perspective on the respiratory protection offered by n-butanol extract from Folium isatidis in LPS-induced sepsis and outline a novel therapeutic strategy for the treatment of sepsis.

No MeSH data available.


Related in: MedlinePlus