Limits...
n-Butanol extract from Folium isatidis inhibits lipopolysaccharide-induced inflammatory cytokine production in macrophages and protects mice against lipopolysaccharide-induced endotoxic shock.

Jiang L, Lu Y, Jin J, Dong L, Xu F, Chen S, Wang Z, Liang G, Shan X - Drug Des Devel Ther (2015)

Bottom Line: Studies have shown beneficial pharmacological effects for Folium isatidis.The present study further illuminated the effects of n-butanol extract from Folium isatidis in LPS-induced septic shock and identified the main active chemical components.Additionally, the extract exhibited dramatic protective effects against lung injury and death in mice with septic shock.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, People's Republic of China.

ABSTRACT
Sepsis, which is caused by severe infection, is an important cause of mortality, but effective clinical treatment against sepsis is extremely limited. As the main component of the outer membrane of Gram-negative bacteria, lipopolysaccharide (LPS) plays a major role in inflammatory responses. Studies have shown beneficial pharmacological effects for Folium isatidis. The present study further illuminated the effects of n-butanol extract from Folium isatidis in LPS-induced septic shock and identified the main active chemical components. Our study showed that pretreatment with n-butanol extract from Folium isatidis not only significantly inhibited LPS-induced tumor necrosis factor-α and interleukin-6 production but also markedly and dose dependently enhanced the recruitment of MyD88, the phosphorylation of extracellular signal-regulated kinase, and the degradation of IκB-α. Additionally, the extract exhibited dramatic protective effects against lung injury and death in mice with septic shock. Eight main active compounds were identified, including organic acids, glycoside, indolinones, and flavonoids. These findings provide a perspective on the respiratory protection offered by n-butanol extract from Folium isatidis in LPS-induced sepsis and outline a novel therapeutic strategy for the treatment of sepsis.

No MeSH data available.


Related in: MedlinePlus

The n-butanol extract from Folium isatidis inhibited LPS-induced inflammatory cytokine expression in mouse macrophages (A and B).Notes: Following pretreatment with vehicle control (DMSO) or a different concentration of the n-butanol extract for 2 hours, MPMs were stimulated with LPS (0.5 µg/mL) for 22 hours. The protein levels of the inflammatory cytokines TNF-α (A) and IL-6 (B) in the culture medium were measured using the ELISA method. Statistical significance compared with the LPS group is indicated, **P<0.01, ***P<0.001.Abbreviations: ELISA, enzyme-linked immunosorbent assay; IL, interleukin; LPS, lipopolysaccharide; MPM, mouse peritoneal macrophage; TNF-α, tumor necrosis factor-α.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4608600&req=5

f2-dddt-9-5601: The n-butanol extract from Folium isatidis inhibited LPS-induced inflammatory cytokine expression in mouse macrophages (A and B).Notes: Following pretreatment with vehicle control (DMSO) or a different concentration of the n-butanol extract for 2 hours, MPMs were stimulated with LPS (0.5 µg/mL) for 22 hours. The protein levels of the inflammatory cytokines TNF-α (A) and IL-6 (B) in the culture medium were measured using the ELISA method. Statistical significance compared with the LPS group is indicated, **P<0.01, ***P<0.001.Abbreviations: ELISA, enzyme-linked immunosorbent assay; IL, interleukin; LPS, lipopolysaccharide; MPM, mouse peritoneal macrophage; TNF-α, tumor necrosis factor-α.

Mentions: To examine whether the extract could affect the inflammatory response induced by LPS, the production of the pro-inflammatory cytokines TNF-α and IL-6 in the presence or absence of the extract was measured using ELISA. As shown in Figure 2, LPS-treated MPMs showed a significant increase in the secretion of TNF-α and IL-6 in the culture media after LPS stimulation, whereas the levels of TNF-α and IL-6 were significantly inhibited by the n-butanol extract from Folium isatidis compared with the levels in the LPS group (P<0.01). Furthermore, improved anti-inflammatory function could be achieved by increasing the drug concentration, indicating that the n-butanol extract from Folium isatidis possesses therapeutic effects against LPS-induced inflammatory responses.


n-Butanol extract from Folium isatidis inhibits lipopolysaccharide-induced inflammatory cytokine production in macrophages and protects mice against lipopolysaccharide-induced endotoxic shock.

Jiang L, Lu Y, Jin J, Dong L, Xu F, Chen S, Wang Z, Liang G, Shan X - Drug Des Devel Ther (2015)

The n-butanol extract from Folium isatidis inhibited LPS-induced inflammatory cytokine expression in mouse macrophages (A and B).Notes: Following pretreatment with vehicle control (DMSO) or a different concentration of the n-butanol extract for 2 hours, MPMs were stimulated with LPS (0.5 µg/mL) for 22 hours. The protein levels of the inflammatory cytokines TNF-α (A) and IL-6 (B) in the culture medium were measured using the ELISA method. Statistical significance compared with the LPS group is indicated, **P<0.01, ***P<0.001.Abbreviations: ELISA, enzyme-linked immunosorbent assay; IL, interleukin; LPS, lipopolysaccharide; MPM, mouse peritoneal macrophage; TNF-α, tumor necrosis factor-α.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4608600&req=5

f2-dddt-9-5601: The n-butanol extract from Folium isatidis inhibited LPS-induced inflammatory cytokine expression in mouse macrophages (A and B).Notes: Following pretreatment with vehicle control (DMSO) or a different concentration of the n-butanol extract for 2 hours, MPMs were stimulated with LPS (0.5 µg/mL) for 22 hours. The protein levels of the inflammatory cytokines TNF-α (A) and IL-6 (B) in the culture medium were measured using the ELISA method. Statistical significance compared with the LPS group is indicated, **P<0.01, ***P<0.001.Abbreviations: ELISA, enzyme-linked immunosorbent assay; IL, interleukin; LPS, lipopolysaccharide; MPM, mouse peritoneal macrophage; TNF-α, tumor necrosis factor-α.
Mentions: To examine whether the extract could affect the inflammatory response induced by LPS, the production of the pro-inflammatory cytokines TNF-α and IL-6 in the presence or absence of the extract was measured using ELISA. As shown in Figure 2, LPS-treated MPMs showed a significant increase in the secretion of TNF-α and IL-6 in the culture media after LPS stimulation, whereas the levels of TNF-α and IL-6 were significantly inhibited by the n-butanol extract from Folium isatidis compared with the levels in the LPS group (P<0.01). Furthermore, improved anti-inflammatory function could be achieved by increasing the drug concentration, indicating that the n-butanol extract from Folium isatidis possesses therapeutic effects against LPS-induced inflammatory responses.

Bottom Line: Studies have shown beneficial pharmacological effects for Folium isatidis.The present study further illuminated the effects of n-butanol extract from Folium isatidis in LPS-induced septic shock and identified the main active chemical components.Additionally, the extract exhibited dramatic protective effects against lung injury and death in mice with septic shock.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, People's Republic of China.

ABSTRACT
Sepsis, which is caused by severe infection, is an important cause of mortality, but effective clinical treatment against sepsis is extremely limited. As the main component of the outer membrane of Gram-negative bacteria, lipopolysaccharide (LPS) plays a major role in inflammatory responses. Studies have shown beneficial pharmacological effects for Folium isatidis. The present study further illuminated the effects of n-butanol extract from Folium isatidis in LPS-induced septic shock and identified the main active chemical components. Our study showed that pretreatment with n-butanol extract from Folium isatidis not only significantly inhibited LPS-induced tumor necrosis factor-α and interleukin-6 production but also markedly and dose dependently enhanced the recruitment of MyD88, the phosphorylation of extracellular signal-regulated kinase, and the degradation of IκB-α. Additionally, the extract exhibited dramatic protective effects against lung injury and death in mice with septic shock. Eight main active compounds were identified, including organic acids, glycoside, indolinones, and flavonoids. These findings provide a perspective on the respiratory protection offered by n-butanol extract from Folium isatidis in LPS-induced sepsis and outline a novel therapeutic strategy for the treatment of sepsis.

No MeSH data available.


Related in: MedlinePlus