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n-Butanol extract from Folium isatidis inhibits lipopolysaccharide-induced inflammatory cytokine production in macrophages and protects mice against lipopolysaccharide-induced endotoxic shock.

Jiang L, Lu Y, Jin J, Dong L, Xu F, Chen S, Wang Z, Liang G, Shan X - Drug Des Devel Ther (2015)

Bottom Line: Studies have shown beneficial pharmacological effects for Folium isatidis.The present study further illuminated the effects of n-butanol extract from Folium isatidis in LPS-induced septic shock and identified the main active chemical components.Additionally, the extract exhibited dramatic protective effects against lung injury and death in mice with septic shock.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, People's Republic of China.

ABSTRACT
Sepsis, which is caused by severe infection, is an important cause of mortality, but effective clinical treatment against sepsis is extremely limited. As the main component of the outer membrane of Gram-negative bacteria, lipopolysaccharide (LPS) plays a major role in inflammatory responses. Studies have shown beneficial pharmacological effects for Folium isatidis. The present study further illuminated the effects of n-butanol extract from Folium isatidis in LPS-induced septic shock and identified the main active chemical components. Our study showed that pretreatment with n-butanol extract from Folium isatidis not only significantly inhibited LPS-induced tumor necrosis factor-α and interleukin-6 production but also markedly and dose dependently enhanced the recruitment of MyD88, the phosphorylation of extracellular signal-regulated kinase, and the degradation of IκB-α. Additionally, the extract exhibited dramatic protective effects against lung injury and death in mice with septic shock. Eight main active compounds were identified, including organic acids, glycoside, indolinones, and flavonoids. These findings provide a perspective on the respiratory protection offered by n-butanol extract from Folium isatidis in LPS-induced sepsis and outline a novel therapeutic strategy for the treatment of sepsis.

No MeSH data available.


Related in: MedlinePlus

Isolation of the n-butanol extract from Folium isatidis.Notes: Dry leaves of Isatis indigotica Fortune were powdered and partitioned by successively using 95% ethanol, petroleum ether, ethyl acetate, and n-butanol three times. The extract from the n-butanol fraction was collected.
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f1-dddt-9-5601: Isolation of the n-butanol extract from Folium isatidis.Notes: Dry leaves of Isatis indigotica Fortune were powdered and partitioned by successively using 95% ethanol, petroleum ether, ethyl acetate, and n-butanol three times. The extract from the n-butanol fraction was collected.

Mentions: An extract was obtained using the maceration method (Figure 1). Dry leaves (10 kg) were powdered and immersed in 30 L of 95% ethanol at room temperature for 1 week and then filtered. The residue was macerated for another week. Extract residues were then combined and centrifuged to dryness under a rotary evaporator at 59°C, resulting in a brown residue (1,053 g). Part of the extract (350 g) was resuspended in 150 mL of water (7:3; v/v). Once completely dispersed, the homogenized sample was successively loaded into petroleum ether, ethyl acetate, and n-butanol according to the polarity of the solvent, and every solvent was extracted three times. The n-butanol fraction was collected and subsequently dried using a rotary evaporator at 45°C. Finally, the residue was dissolved to an appropriate concentration for pharmacodynamics study and liquid chromatography (LC)–MS/MS injection.


n-Butanol extract from Folium isatidis inhibits lipopolysaccharide-induced inflammatory cytokine production in macrophages and protects mice against lipopolysaccharide-induced endotoxic shock.

Jiang L, Lu Y, Jin J, Dong L, Xu F, Chen S, Wang Z, Liang G, Shan X - Drug Des Devel Ther (2015)

Isolation of the n-butanol extract from Folium isatidis.Notes: Dry leaves of Isatis indigotica Fortune were powdered and partitioned by successively using 95% ethanol, petroleum ether, ethyl acetate, and n-butanol three times. The extract from the n-butanol fraction was collected.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4608600&req=5

f1-dddt-9-5601: Isolation of the n-butanol extract from Folium isatidis.Notes: Dry leaves of Isatis indigotica Fortune were powdered and partitioned by successively using 95% ethanol, petroleum ether, ethyl acetate, and n-butanol three times. The extract from the n-butanol fraction was collected.
Mentions: An extract was obtained using the maceration method (Figure 1). Dry leaves (10 kg) were powdered and immersed in 30 L of 95% ethanol at room temperature for 1 week and then filtered. The residue was macerated for another week. Extract residues were then combined and centrifuged to dryness under a rotary evaporator at 59°C, resulting in a brown residue (1,053 g). Part of the extract (350 g) was resuspended in 150 mL of water (7:3; v/v). Once completely dispersed, the homogenized sample was successively loaded into petroleum ether, ethyl acetate, and n-butanol according to the polarity of the solvent, and every solvent was extracted three times. The n-butanol fraction was collected and subsequently dried using a rotary evaporator at 45°C. Finally, the residue was dissolved to an appropriate concentration for pharmacodynamics study and liquid chromatography (LC)–MS/MS injection.

Bottom Line: Studies have shown beneficial pharmacological effects for Folium isatidis.The present study further illuminated the effects of n-butanol extract from Folium isatidis in LPS-induced septic shock and identified the main active chemical components.Additionally, the extract exhibited dramatic protective effects against lung injury and death in mice with septic shock.

View Article: PubMed Central - PubMed

Affiliation: Department of Pediatrics, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, People's Republic of China.

ABSTRACT
Sepsis, which is caused by severe infection, is an important cause of mortality, but effective clinical treatment against sepsis is extremely limited. As the main component of the outer membrane of Gram-negative bacteria, lipopolysaccharide (LPS) plays a major role in inflammatory responses. Studies have shown beneficial pharmacological effects for Folium isatidis. The present study further illuminated the effects of n-butanol extract from Folium isatidis in LPS-induced septic shock and identified the main active chemical components. Our study showed that pretreatment with n-butanol extract from Folium isatidis not only significantly inhibited LPS-induced tumor necrosis factor-α and interleukin-6 production but also markedly and dose dependently enhanced the recruitment of MyD88, the phosphorylation of extracellular signal-regulated kinase, and the degradation of IκB-α. Additionally, the extract exhibited dramatic protective effects against lung injury and death in mice with septic shock. Eight main active compounds were identified, including organic acids, glycoside, indolinones, and flavonoids. These findings provide a perspective on the respiratory protection offered by n-butanol extract from Folium isatidis in LPS-induced sepsis and outline a novel therapeutic strategy for the treatment of sepsis.

No MeSH data available.


Related in: MedlinePlus