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Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA.

Reddy TL, Krishnarao PS, Rao GK, Bhimireddy E, Venkateswarlu P, Mohapatra DK, Yadav JS, Bhadra U, Bhadra MP - Int J Nanomedicine (2015)

Bottom Line: A number of diseases can result from abnormal gene expression.Our findings indicated high gene transfection efficiency.These biocompatible nanoparticles allow targeted delivery of siRNA, providing an efficient vehicle for gene delivery.

View Article: PubMed Central - PubMed

Affiliation: Centre for Chemical Biology, CSIR-Indian Institute of Chemical Technology, Hyderabad, India ; Academy of Scientific and Innovative Research, New Delhi, India.

ABSTRACT
A number of diseases can result from abnormal gene expression. One of the approaches for treating such diseases is gene therapy to inhibit expression of a particular gene in a specific cell population by RNA interference. Use of efficient delivery vehicles increases the safety and success of gene therapy. Here we report the development of functionalized biocompatible fluorescent nanoparticles from para amino benzoic acid nanoparticles for efficient delivery of short interfering RNA (siRNA). These nanoparticles were non-toxic and did not interfere with progression of the cell cycle. The intrinsic fluorescent nature of these nanoparticles allows easy tracking and an opportunity for diagnostic applications. Human Bcl-2 siRNA was complexed with these nanoparticles to inhibit expression in cells at both the transcriptional and translational levels. Our findings indicated high gene transfection efficiency. These biocompatible nanoparticles allow targeted delivery of siRNA, providing an efficient vehicle for gene delivery.

No MeSH data available.


Related in: MedlinePlus

RFP silencing in MDA-MB-231/RFP cells. Confocal microscopic images of MDA-MB-231/RFP cells after 48 hours post transfection with NP-RFP-siRNA complexes. The nucleus is stained with DAPI. Scale 20 μm.Abbreviations: NP, nanoparticle; siRNA, short interfering RNA; DAPI, 4,6-diamidino-2-phenylindole; RFP, red fluorescent protein.
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f6-ijn-10-6411: RFP silencing in MDA-MB-231/RFP cells. Confocal microscopic images of MDA-MB-231/RFP cells after 48 hours post transfection with NP-RFP-siRNA complexes. The nucleus is stained with DAPI. Scale 20 μm.Abbreviations: NP, nanoparticle; siRNA, short interfering RNA; DAPI, 4,6-diamidino-2-phenylindole; RFP, red fluorescent protein.

Mentions: To further visualize the silencing efficiency of the NP-siRNA complexes, we transfected MDA-MB-231/RFP cells with NPs complexed siRNA against RFP. Forty-eight hours post transfection, the cells were visualized under confocal microscopy. The amount of silencing was compared with non-transfected controls. Simultaneously, Lipofectamine RNAiMAX complexed siRNA against RFP was taken as positive control. All the NPs complexed to RFP-siRNA (100 nM) significantly and potently silenced RFP expression by more than 80% when compared with untreated controls (Figure 6).


Para amino benzoic acid-derived self-assembled biocompatible nanoparticles for efficient delivery of siRNA.

Reddy TL, Krishnarao PS, Rao GK, Bhimireddy E, Venkateswarlu P, Mohapatra DK, Yadav JS, Bhadra U, Bhadra MP - Int J Nanomedicine (2015)

RFP silencing in MDA-MB-231/RFP cells. Confocal microscopic images of MDA-MB-231/RFP cells after 48 hours post transfection with NP-RFP-siRNA complexes. The nucleus is stained with DAPI. Scale 20 μm.Abbreviations: NP, nanoparticle; siRNA, short interfering RNA; DAPI, 4,6-diamidino-2-phenylindole; RFP, red fluorescent protein.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4608593&req=5

f6-ijn-10-6411: RFP silencing in MDA-MB-231/RFP cells. Confocal microscopic images of MDA-MB-231/RFP cells after 48 hours post transfection with NP-RFP-siRNA complexes. The nucleus is stained with DAPI. Scale 20 μm.Abbreviations: NP, nanoparticle; siRNA, short interfering RNA; DAPI, 4,6-diamidino-2-phenylindole; RFP, red fluorescent protein.
Mentions: To further visualize the silencing efficiency of the NP-siRNA complexes, we transfected MDA-MB-231/RFP cells with NPs complexed siRNA against RFP. Forty-eight hours post transfection, the cells were visualized under confocal microscopy. The amount of silencing was compared with non-transfected controls. Simultaneously, Lipofectamine RNAiMAX complexed siRNA against RFP was taken as positive control. All the NPs complexed to RFP-siRNA (100 nM) significantly and potently silenced RFP expression by more than 80% when compared with untreated controls (Figure 6).

Bottom Line: A number of diseases can result from abnormal gene expression.Our findings indicated high gene transfection efficiency.These biocompatible nanoparticles allow targeted delivery of siRNA, providing an efficient vehicle for gene delivery.

View Article: PubMed Central - PubMed

Affiliation: Centre for Chemical Biology, CSIR-Indian Institute of Chemical Technology, Hyderabad, India ; Academy of Scientific and Innovative Research, New Delhi, India.

ABSTRACT
A number of diseases can result from abnormal gene expression. One of the approaches for treating such diseases is gene therapy to inhibit expression of a particular gene in a specific cell population by RNA interference. Use of efficient delivery vehicles increases the safety and success of gene therapy. Here we report the development of functionalized biocompatible fluorescent nanoparticles from para amino benzoic acid nanoparticles for efficient delivery of short interfering RNA (siRNA). These nanoparticles were non-toxic and did not interfere with progression of the cell cycle. The intrinsic fluorescent nature of these nanoparticles allows easy tracking and an opportunity for diagnostic applications. Human Bcl-2 siRNA was complexed with these nanoparticles to inhibit expression in cells at both the transcriptional and translational levels. Our findings indicated high gene transfection efficiency. These biocompatible nanoparticles allow targeted delivery of siRNA, providing an efficient vehicle for gene delivery.

No MeSH data available.


Related in: MedlinePlus